Open Access

Long non‑coding RNA HOTAIRM1‑1 silencing in cartilage tissue induces osteoarthritis through microRNA‑125b

  • Authors:
    • Wen-Bin Liu
    • Qi-Jin Feng
    • Gui-Shi Li
    • Peng Shen
    • Ya-Nan Li
    • Fu-Jiang Zhang
  • View Affiliations

  • Published online on: July 1, 2021     https://doi.org/10.3892/etm.2021.10365
  • Article Number: 933
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Aberrations in long noncoding RNA (lncRNA) expression have been recognized in numerous human diseases. In the present study, the of role the long noncoding RNA HOX antisense intergenic RNA myeloid 1 variant (HOTAIRM1‑1) in regulating the pathological progression of osteoarthritis (OA) was investigated. The aberrant expression of HOTAIRM1‑1 in OA was demonstrated, but the molecular mechanisms require further analysis. The aim of the present study was to explore the function of miR‑125b in modulating chondrocyte viability and apoptosis, and to address the functional association between HOTAIRM1‑1 and miR‑125b as potential targets. A miR‑125b inhibitor was used, which laid the foundation for the following investigation. The study confirmed that HOTAIRM1‑1 and miR‑125b are inversely expressed in chondrocytes. The expression of HOTAIRM1‑1 was downregulated and the expression of miR‑125b was upregulated in tissues from patients with OA. HOTAIRM1‑1 directly interacted with miR‑125b in chondrocytes. HOTAIRM1‑1 knockdown was associated with chondrocyte proliferation and extracellular matrix degradation. Furthermore, miR‑125b reversed the effect of HOTAIRM1‑1 on cell proliferation and apoptosis. In conclusion, the present study indicates that the loss of HOTAIRM1‑1 function leads to aberrant increases in the proliferation and apoptosis of chondrocytes. miR‑125b may be a potential downstream mechanism that regulates the function of HOTAIRM1‑1, and this finding provides a therapeutic strategy for OA.
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September-2021
Volume 22 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu W, Feng Q, Li G, Shen P, Li Y and Zhang F: Long non‑coding RNA HOTAIRM1‑1 silencing in cartilage tissue induces osteoarthritis through microRNA‑125b. Exp Ther Med 22: 933, 2021
APA
Liu, W., Feng, Q., Li, G., Shen, P., Li, Y., & Zhang, F. (2021). Long non‑coding RNA HOTAIRM1‑1 silencing in cartilage tissue induces osteoarthritis through microRNA‑125b. Experimental and Therapeutic Medicine, 22, 933. https://doi.org/10.3892/etm.2021.10365
MLA
Liu, W., Feng, Q., Li, G., Shen, P., Li, Y., Zhang, F."Long non‑coding RNA HOTAIRM1‑1 silencing in cartilage tissue induces osteoarthritis through microRNA‑125b". Experimental and Therapeutic Medicine 22.3 (2021): 933.
Chicago
Liu, W., Feng, Q., Li, G., Shen, P., Li, Y., Zhang, F."Long non‑coding RNA HOTAIRM1‑1 silencing in cartilage tissue induces osteoarthritis through microRNA‑125b". Experimental and Therapeutic Medicine 22, no. 3 (2021): 933. https://doi.org/10.3892/etm.2021.10365