Long non‑coding RNA HOTAIRM1‑1 silencing in cartilage tissue induces osteoarthritis through microRNA‑125b Corrigendum in /10.3892/etm.2022.11327

Liu,Wen-Bin; Li,Gui-Shi; Shen,Peng; Li,Ya-Nan; Zhang,Fu-Jiang

doi:10.3892/etm.2021.10365

Long non‑coding RNA HOTAIRM1‑1 silencing in cartilage tissue induces osteoarthritis through microRNA‑125b

Corrigendum in: /10.3892/etm.2022.11327

Authors:
- Wen-Bin Liu
- Gui-Shi Li
- Peng Shen
- Ya-Nan Li
- Fu-Jiang Zhang
View Affiliations

Affiliations: Department of Joint Surgery, Tianjin Hospital, Tianjin 300211, P.R. China, Department of Joint Surgery, Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China, Department of Rheumatology and Immunology, Tianjin First Center Hospital, Tianjin 300192, P.R. China, Department of Orthopedics, Tianjin Dongli Hospital, Tianjin 300300, P.R. China
Published online on: July 1, 2021 https://doi.org/10.3892/etm.2021.10365
Article Number: 933
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Aberrations in long noncoding RNA (lncRNA) expression have been recognized in numerous human diseases. In the present study, the of role the long noncoding RNA HOX antisense intergenic RNA myeloid 1 variant (HOTAIRM1‑1) in regulating the pathological progression of osteoarthritis (OA) was investigated. The aberrant expression of HOTAIRM1‑1 in OA was demonstrated, but the molecular mechanisms require further analysis. The aim of the present study was to explore the function of miR‑125b in modulating chondrocyte viability and apoptosis, and to address the functional association between HOTAIRM1‑1 and miR‑125b as potential targets. A miR‑125b inhibitor was used, which laid the foundation for the following investigation. The study confirmed that HOTAIRM1‑1 and miR‑125b are inversely expressed in chondrocytes. The expression of HOTAIRM1‑1 was downregulated and the expression of miR‑125b was upregulated in tissues from patients with OA. HOTAIRM1‑1 directly interacted with miR‑125b in chondrocytes. HOTAIRM1‑1 knockdown was associated with chondrocyte proliferation and extracellular matrix degradation. Furthermore, miR‑125b reversed the effect of HOTAIRM1‑1 on cell proliferation and apoptosis. In conclusion, the present study indicates that the loss of HOTAIRM1‑1 function leads to aberrant increases in the proliferation and apoptosis of chondrocytes. miR‑125b may be a potential downstream mechanism that regulates the function of HOTAIRM1‑1, and this finding provides a therapeutic strategy for OA.

View Figures

View References

1	Thomas AC, Hubbard-Turner T, Wikstrom EA and Palmieri-Smith RM: Epidemiology of posttraumatic osteoarthritis. J Athl Train. 52:491–496. 2017.PubMed/NCBI View Article : Google Scholar
2	Vina ER and Kwoh CK: Epidemiology of osteoarthritis: Literature update. Curr Opin Rheumatol. 30:160–167. 2018.PubMed/NCBI View Article : Google Scholar
3	Okada A and Okada Y: Progress of research in osteoarthritis. Metalloproteinases in osteoarthritis. Clin Calcium. 19:1593–1601. 2009.PubMed/NCBI(In Japanese).
4	Yoon DS, Lee KM, Kim SH, Kim SH, Jung Y, Kim SH, Park KH, Choi Y, Ryu HA, Choi WJ and Lee JW: Synergistic action of IL-8 and bone marrow concentrate on cartilage regeneration through upregulation of chondrogenic transcription factors. Tissue Eng Part A. 22:363–374. 2016.PubMed/NCBI View Article : Google Scholar
5	Richardson SM, Kalamegam G, Pushparaj PN, Matta C, Memic A, Khademhosseini A, Mobasheri R, Poletti FL, Hoyland JA and Mobasheri A: Mesenchymal stem cells in regenerative medicine: Focus on articular cartilage and intervertebral disc regeneration. Methods. 99:69–80. 2016.PubMed/NCBI View Article : Google Scholar
6	Mobasheri A, Kalamegam G, Musumeci G and Batt ME: Chondrocyte and mesenchymal stem cell-based therapies for cartilage repair in osteoarthritis and related orthopaedic conditions. Maturitas. 78:188–198. 2014.PubMed/NCBI View Article : Google Scholar
7	Pant T, Dhanasekaran A, Fang J, Bai X, Bosnjak ZJ, Liang M and Ge ZD: Current status and strategies of long noncoding RNA research for diabetic cardiomyopathy. BMC Cardiovasc Disord. 18(197)2018.PubMed/NCBI View Article : Google Scholar
8	Ji D, Zhong X, Jiang X, Leng K, Xu Y, Li Z, Huang L, Li J and Cui Y: The role of long non-coding RNA AFAP1-AS1 in human malignant tumors. Pathol Res Pract. 214:1524–1531. 2018.PubMed/NCBI View Article : Google Scholar
9	Jiang SD, Lu J, Deng ZH, Li YS and Lei GH: Long noncoding RNAs in osteoarthritis. Joint Bone Spine. 84:553–556. 2017.PubMed/NCBI View Article : Google Scholar
10	Marques-Rocha JL, Samblas M, Milagro FI, Bressan J, Martinez JA and Marti A: Noncoding RNAs, cytokines, and inflammation-related diseases. FASEB J. 29:3595–3611. 2015.PubMed/NCBI View Article : Google Scholar
11	Chen L, Hu N, Wang C and Zhao H: HOTAIRM1 knockdown enhances cytarabine-induced cytotoxicity by suppression of glycolysis through the Wnt/β-catenin/PFKP pathway in acute myeloid leukemia cells. Arch Biochem Biophys. 680(108244)2020.PubMed/NCBI View Article : Google Scholar
12	Bhan A, Hussain I, Ansari KI, Kasiri S, Bashyal A and Mandal SS: Antisense transcript long noncoding RNA (lncRNA) HOTAIR is transcriptionally induced by estradiol. J Mol Biol. 425:3707–3722. 2013.PubMed/NCBI View Article : Google Scholar
13	Wang Y, Hardin H, Chu YH, Esbona K, Zhang R and Lloyd RV: Long non-coding RNA expression in anaplastic thyroid carcinomas. Endocr Pathol. 30:262–269. 2019.PubMed/NCBI View Article : Google Scholar
14	Xiao Y, Yan X, Yang Y and Ma X: Downregulation of long noncoding RNA HOTAIRM1 variant 1 contributes to osteoarthritis via regulating miR-125b/BMPR2 axis and activating JNK/MAPK/ERK pathway. Biomed Pharmacother. 109:1569–1577. 2019.PubMed/NCBI View Article : Google Scholar
15	Wei S, Zhao M, Wang X, Li Y and Wang K: PU.1 controls the expression of long noncoding RNA HOTAIRM1 during granulocytic differentiation. J Hematol Oncol. 9(44)2016.PubMed/NCBI View Article : Google Scholar
16	Li Y, Li S, Luo Y, Liu Y and Yu N: LncRNA PVT1 Regulates chondrocyte apoptosis in osteoarthritis by acting as a sponge for miR-488-3p. DNA Cell Biol. 36:571–580. 2017.PubMed/NCBI View Article : Google Scholar
17	Huang K, Fu J, Zhou W, Li W, Dong S, Yu S, Hu Z, Wang H and Xie Z: MicroRNA-125b regulates osteogenic differentiation of mesenchymal stem cells by targeting Cbfβ in vitro. Biochimie. 102:47–55. 2014.PubMed/NCBI View Article : Google Scholar
18	Zhang HF, Li ZJ, Fu X, Ma JX and Ma XL: Interactions of bone marrow stromal cells with native and RGD surface modified acellular bone matrix: A biocompatibility study. Arch Med Res. 44:69–74. 2013.PubMed/NCBI View Article : Google Scholar
19	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar
20	Li L, Wang Y, Song G, Zhang X, Gao S and Liu H: HOX cluster-embedded antisense long non-coding RNAs in lung cancer. Cancer Lett. 450:14–21. 2019.PubMed/NCBI View Article : Google Scholar
21	Gulei D, Mehterov N, Ling H, Stanta G, Braicu C and Berindan-Neagoe I: The ‘good-cop bad-cop’ TGF-β role in breast cancer modulated by non-coding RNAs. Biochim Biophys Acta Gen Subj. 1861:1661–1675. 2017.PubMed/NCBI View Article : Google Scholar
22	Park S, Lee M, Chun CH and Jin EJ: The lncRNA, nespas, is associated with osteoarthritis progression and serves as a potential new prognostic biomarker. Cartilage. 10:148–156. 2019.PubMed/NCBI View Article : Google Scholar
23	Yang S, Kim J, Ryu JH, Oh H, Chun CH, Kim BJ, Min BH and Chun JS: Hypoxia-inducible factor-2alpha is a catabolic regulator of osteoarthritic cartilage destruction. Nat Med. 16:687–693. 2010.PubMed/NCBI View Article : Google Scholar
24	Miyaki S, Sato T, Inoue A, Otsuki S, Ito Y, Yokoyama S, Kato Y, Takemoto F, Nakasa T, Yamashita S, et al: MicroRNA-140 plays dual roles in both cartilage development and homeostasis. Genes Dev. 24:1173–1185. 2010.PubMed/NCBI View Article : Google Scholar
25	Wojdasiewicz P, Poniatowski LA and Szukiewicz D: The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of osteoarthritis. Mediators Inflamm. 2014(561459)2014.PubMed/NCBI View Article : Google Scholar
26	Pearson MJ, Herndler-Brandstetter D, Tariq MA, Nicholson TA, Philp AM, Smith HL, Davis ET, Jones SW and Lord JM: IL-6 secretion in osteoarthritis patients is mediated by chondrocyte-synovial fibroblast cross-talk and is enhanced by obesity. Sci Rep. 7(1)(3451)2017.PubMed/NCBI View Article : Google Scholar
27	Zanotti S and Canalis E: Interleukin 6 mediates selected effects of notch in chondrocytes. Osteoarthritis Cartilage. 21:1766–1773. 2013.PubMed/NCBI View Article : Google Scholar
28	Simsa-Maziel S and Monsonego-Ornan E: Interleukin-1β promotes proliferation and inhibits differentiation of chondrocytes through a mechanism involving down-regulation of FGFR-3 and p21. Endocrinology. 153:2296–2310. 2012.PubMed/NCBI View Article : Google Scholar
29	Cheng NL, Chen X, Kim J, Shi AH, Nguyen C, Wersto R and Weng NP: MicroRNA-125b modulates inflammatory chemokine CCL4 expression in immune cells and its reduction causes CCL4 increase with age. Aging Cell. 14:200–208. 2015.PubMed/NCBI View Article : Google Scholar
30	Nagpal V, Rai R, Place AT, Murphy SB, Verma SK, Ghosh AK and Vaughan DE: MiR-125b is critical for fibroblast-to-myofibroblast transition and cardiac fibrosis. Circulation. 133:291–301. 2016.PubMed/NCBI View Article : Google Scholar
31	Zhen G, Wen C, Jia X, Li Y, Crane JL, Mears SC, Askin FB, Frassica FJ, Chang W, Yao J, et al: Inhibition of TGF-β signaling in mesenchymal stem cells of subchondral bone attenuates osteoarthritis. Nat Med. 19:704–712. 2013.PubMed/NCBI View Article : Google Scholar
32	Shi X, Shao X, Liu B, Lv M, Pandey P, Guo C, Zhang R and Zhang Y: Genome-wide screening of functional long noncoding RNAs in the epicardial adipose tissues of atrial fibrillation. Biochim Biophys Acta Mol Basis Dis. 1866(165757)2020.PubMed/NCBI View Article : Google Scholar
33	Charlier E, Deroyer C, Ciregia F, Malaise O, Neuville S, Plener Z, Malaise M and de Seny D: Chondrocyte dedifferentiation and osteoarthritis (OA). Biochem Pharmacol. 165:49–65. 2019.PubMed/NCBI View Article : Google Scholar
34	Silawal S, Willauschus M, Schulze-Tanzil G, Gogele C, Gesslein M and Schwarz S: IL-10 could play a role in the interrelation between diabetes mellitus and osteoarthritis. Int J Mol Sci. 20(768)2019.PubMed/NCBI View Article : Google Scholar
35	Hu J, Wang Z, Shan Y, Pan Y, Ma J and Jia L: Long non-coding RNA HOTAIR promotes osteoarthritis progression via miR-17-5p/FUT2/β-catenin axis. Cell Death Dis. 9(711)2018.PubMed/NCBI View Article : Google Scholar
36	Matyas JR, Adams ME, Huang D and Sandell LJ: Discoordinate gene expression of aggrecan and type II collagen in experimental osteoarthritis. Arthritis Rheum. 38:420–425. 1995.PubMed/NCBI View Article : Google Scholar
37	Misso G, Zarone MR, Lombardi A, Grimaldi A, Cossu AM, Ferri C, Russo M, Vuoso DC, Luce A, Kawasaki H, et al: miR-125b upregulates miR-34a and sequentially activates stress adaption and cell death mechanisms in multiple myeloma. Mol Ther Nucleic Acids. 16:391–406. 2019.PubMed/NCBI View Article : Google Scholar
38	Cui F, Li X, Zhu X, Huang L, Huang Y, Mao C, Yan Q, Zhu J, Zhao W and Shi H: MiR-125b inhibits tumor growth and promotes apoptosis of cervical cancer cells by targeting phosphoinositide 3-kinase catalytic subunit delta. Cell Physiol Biochem. 30:1310–1318. 2012.PubMed/NCBI View Article : Google Scholar