Comparative study of three types of mesenchymal stem cell to differentiate into pancreatic β‑like cells <em>in vitro</em>

Mo,Yunfang; Wang,Zejian; Gao,Jian; Yan,Yan; Ren,Huaijuan; Zhang,Fengli; Qi,Nianmin; Chen,Yantian

doi:10.3892/etm.2021.10368

Comparative study of three types of mesenchymal stem cell to differentiate into pancreatic β‑like cells in vitro

Authors:
- Yunfang Mo
- Zejian Wang
- Jian Gao
- Yan Yan
- Huaijuan Ren
- Fengli Zhang
- Nianmin Qi
- Yantian Chen
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Affiliations: Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, P.R. China, China Stem Cell Therapy Co., Ltd., Shanghai 201203, P.R. China
Published online on: July 1, 2021 https://doi.org/10.3892/etm.2021.10368
Article Number: 936
Copyright: © Mo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Type 1 diabetes (TID) is a chronic metabolic disease where the body produces insufficient or no insulin. Stem cells with multi‑directional differentiation potential are transplanted and differentiate into β‑like cells in vivo to replace pancreatic β cells, which has become a novel treatment strategy. The aim of the present study was to investigate the ability of three types of adult mesenchymal stem cell (MSC) to differentiate into pancreatic β‑like cells in vitro in order to identify suitable sources for the treatment of diabetes. The three MSC types were menstrual blood‑derived MSCs (MENSCs), umbilical cord‑derived MSCs (UCMSCs) and dental pulp MSCs (DPSCs). The differentiation method used in the present study was divided into three steps and the MSCs were differentiated into pancreatic β‑like cells in vitro. Among these MSCs, MENSCs had a greater ability to differentiate into islet β‑like cells in vitro, while UCMSCs and DPSCs exhibited a similar differentiation potency, which was relatively lower compared with that of MENSCs. The present results indicated that MENSCs may be a suitable cell source for the curative treatment of TID.

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