MicroRNA‑146a overexpression alleviates intestinal ischemia/reperfusion‑induced acute lung injury in mice

Li,Gehui; Xu,Min; Wang,Hao; Qi,Xiaofei; Wang,Xiaoguang; Li,Yong; Sun,Jing; Li,Yuantao

doi:10.3892/etm.2021.10369

MicroRNA‑146a overexpression alleviates intestinal ischemia/reperfusion‑induced acute lung injury in mice

Authors:
- Gehui Li
- Min Xu
- Hao Wang
- Xiaofei Qi
- Xiaoguang Wang
- Yong Li
- Jing Sun
- Yuantao Li
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Affiliations: Department of Anesthesiology, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, Guangdong 518028, P.R. China, Department of Anesthesiology, Xiangyang No.1 People's Hospital, Hubei University of Medicine, Xiangyang, Hubei 441000, P.R. China, Department of Food Safety, Market Supervision Administration of Shenzhen Municipality, Shenzhen, Guangdong 518040, P.R. China
Published online on: July 1, 2021 https://doi.org/10.3892/etm.2021.10369
Article Number: 937
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have shown that microRNAs (miRs), such as miR‑146a play an important role in the pathogenesis of intestinal ischemia/reperfusion (I/R)‑induced injury; however, the role of miR‑146a in intestinal I/R‑induced acute lung injury has not been elucidated. An intestinal I/R‑induced injury mouse model was established in the present study by clamping the superior mesenteric artery and expression levels of miR‑146a in intestinal and lung tissue samples were evaluated using reverse transcription‑quantitative PCR (RT‑qPCR). Intestinal and lung histopathological characteristics in mice with intestinal I/R‑induced injury were assessed by hematoxylin and eosin staining, and mRNA and protein expression levels in intestinal and lung tissue samples were evaluated using RT‑qPCR and western blotting, respectively. miR‑146a expression was significantly downregulated in the intestinal and lung tissue samples of mice with intestinal I/R‑induced injury. Intestinal I/R injury‑induced histopathological changes in the lung and intestines, and pulmonary edema in mice transduced with an adenoviral miR‑146a‑overexpression vector (the miR‑146a overexpression group) were alleviated. mRNA expression levels of TNF‑α, IL‑1β, IFN‑γ and TGF‑β1, and protein expression levels of TNF receptor‑associated factor 6, phosphorylated‑p65 NF‑κB, cleaved caspase‑3 and cleaved caspase‑9 in lung and intestinal tissue samples were downregulated in I/R‑miR‑146a‑overexpressing mice, compared with those from the I/R‑negative control group. Thus, the present study identified that pre‑treatment with the miR‑146a overexpression vector alleviated intestinal I/R‑induced acute lung injury in mice.

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