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MicroRNA‑146a overexpression alleviates intestinal ischemia/reperfusion‑induced acute lung injury in mice

  • Authors:
    • Gehui Li
    • Min Xu
    • Hao Wang
    • Xiaofei Qi
    • Xiaoguang Wang
    • Yong Li
    • Jing Sun
    • Yuantao Li
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, Guangdong 518028, P.R. China, Department of Anesthesiology, Xiangyang No.1 People's Hospital, Hubei University of Medicine, Xiangyang, Hubei 441000, P.R. China, Department of Food Safety, Market Supervision Administration of Shenzhen Municipality, Shenzhen, Guangdong 518040, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 937
    |
    Published online on: July 1, 2021
       https://doi.org/10.3892/etm.2021.10369
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Abstract

Previous studies have shown that microRNAs (miRs), such as miR‑146a play an important role in the pathogenesis of intestinal ischemia/reperfusion (I/R)‑induced injury; however, the role of miR‑146a in intestinal I/R‑induced acute lung injury has not been elucidated. An intestinal I/R‑induced injury mouse model was established in the present study by clamping the superior mesenteric artery and expression levels of miR‑146a in intestinal and lung tissue samples were evaluated using reverse transcription‑quantitative PCR (RT‑qPCR). Intestinal and lung histopathological characteristics in mice with intestinal I/R‑induced injury were assessed by hematoxylin and eosin staining, and mRNA and protein expression levels in intestinal and lung tissue samples were evaluated using RT‑qPCR and western blotting, respectively. miR‑146a expression was significantly downregulated in the intestinal and lung tissue samples of mice with intestinal I/R‑induced injury. Intestinal I/R injury‑induced histopathological changes in the lung and intestines, and pulmonary edema in mice transduced with an adenoviral miR‑146a‑overexpression vector (the miR‑146a overexpression group) were alleviated. mRNA expression levels of TNF‑α, IL‑1β, IFN‑γ and TGF‑β1, and protein expression levels of TNF receptor‑associated factor 6, phosphorylated‑p65 NF‑κB, cleaved caspase‑3 and cleaved caspase‑9 in lung and intestinal tissue samples were downregulated in I/R‑miR‑146a‑overexpressing mice, compared with those from the I/R‑negative control group. Thus, the present study identified that pre‑treatment with the miR‑146a overexpression vector alleviated intestinal I/R‑induced acute lung injury in mice.
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1 

Zhou J, Zimmermann K, Krieg T, Soltow M, Pavlovic D, Cerny V and Lehmann C: Adenosine receptor activation improves microcirculation in experimental intestinal ischemia/reperfusion. Clin Hemorheol Microcirc. 59:257–265. 2015.PubMed/NCBI View Article : Google Scholar

2 

Wu MC, Brennan FH, Lynch JP, Mantovani S, Phipps S, Wetsel RA, Ruitenberg MJ, Taylor SM and Woodruff TM: The receptor for complement component C3a mediates protection from intestinal ischemia-reperfusion injuries by inhibiting neutrophil mobilization. Proc Natl Acad Sci USA. 110:9439–9444. 2013.PubMed/NCBI View Article : Google Scholar

3 

Zhao W, Gan X, Su G, Wanling G, Li S, Hei Z, Yang C and Wang H: The interaction between oxidative stress and mast cell activation plays a role in acute lung injuries induced by intestinal ischemia-reperfusion. J Surg Res. 187:542–552. 2014.PubMed/NCBI View Article : Google Scholar

4 

Rubenfeld GD, Caldwell E, Peabody E, Weaver J, Martin DP, Neff M, Stern EJ and Hudson LD: Incidence and outcomes of acute lung injury. N Engl J Med. 353:1685–1693. 2005.PubMed/NCBI View Article : Google Scholar

5 

Djuranovic S, Nahvi A and Green R: A parsimonious model for gene regulation by miRNAs. Science. 331:550–553. 2011.PubMed/NCBI View Article : Google Scholar

6 

Clark EA, Kalomoiris S, Nolta JA and Fierro FA: Concise review: MicroRNA function in multipotent mesenchymal stromal cells. Stem Cells. 32:1074–1082. 2014.PubMed/NCBI View Article : Google Scholar

7 

Dai Y, Mao Z, Han X, Xu Y, Xu L, Yin L, Qi Y and Peng J: MicroRNA-29b-3p reduces intestinal ischaemia/reperfusion injury via targeting of TNF receptor-associated factor 3. Br J Pharmacol. 176:3264–3278. 2019.PubMed/NCBI View Article : Google Scholar

8 

Liu L, Yao J, Li Z, Zu G, Feng D, Li Y, Qasim W, Zhang S, Li T, Zeng H and Tian X: miR-381-3p knockdown improves intestinal epithelial proliferation and barrier function after intestinal ischemia/reperfusion injury by targeting Nurr1. Cell Death Dis. 9(411)2018.PubMed/NCBI View Article : Google Scholar

9 

Leng C, Sun J, Xin K, Ge J, Liu P and Feng X: High expression of miR-483-5p aggravates sepsis-induced acute lung injury. J Toxicol Sci. 45:77–86. 2020.PubMed/NCBI View Article : Google Scholar

10 

Cao X, Zhang C, Zhang X, Chen Y and Zhang H: MiR-145 negatively regulates TGFBR2 signaling responsible for sepsis-induced acute lung injury. Biomed Pharmacother. 111:852–858. 2019.PubMed/NCBI View Article : Google Scholar

11 

Dai Y, Jia P, Fang Y, Liu H, Jiao X, He JC and Ding X: miR-146a is essential for lipopolysaccharide (LPS)-induced cross-tolerance against kidney ischemia/reperfusion injury in mice. Sci Rep. 6(27091)2016.PubMed/NCBI View Article : Google Scholar

12 

Jiang S, Hu Y, Deng S, Deng J, Yu X, Huang G, Kawai T and Han X: miR-146a regulates inflammatory cytokine production in porphyromonas gingivalis lipopolysaccharide-stimulated B cells by targeting IRAK1 but not TRAF6. Biochim Biophys Acta Mol Basis Dis. 1864:925–933. 2018.PubMed/NCBI View Article : Google Scholar

13 

Zeng Z, Gong H, Li Y, Jie K, Ding C, Shao Q, Liu F, Zhan Y, Nie C, Zhu W and Qian K: Upregulation of miR-146a contributes to the suppression of inflammatory responses in LPS-induced acute lung injury. Exp Lung Res. 39:275–282. 2013.PubMed/NCBI View Article : Google Scholar

14 

Chassin C, Hempel C, Stockinger S, Dupont A, Kübler JF, Wedemeyer J, Vandewalle A and Hornef MW: MicroRNA-146a-mediated downregulation of IRAK1 protects mouse and human small intestine against ischemia/reperfusion injury. EMBO Mol Med. 4:1308–1319. 2012.PubMed/NCBI View Article : Google Scholar

15 

Lv N and Li X: Isoflurane suppresses lung ischemia-reperfusion injury by inactivating NF-κB and inhibiting cell apoptosis. Exp Ther Med. 20(74)2020.PubMed/NCBI View Article : Google Scholar

16 

Liu J, Chen T, Lei P, Tang X and Huang P: Exosomes released by bone marrow mesenchymal stem cells attenuate lung injury induced by intestinal ischemia reperfusion via the TLR4/NF-κB pathway. Int J Med Sci. 16:1238–1244. 2019.PubMed/NCBI View Article : Google Scholar

17 

Shen CH, Lin JY, Chang YL, Wu SY, Peng CK, Wu CP and Huang KL: Inhibition of NKCC1 modulates alveolar fluid clearance and inflammation in ischemia-reperfusion lung injury via TRAF6-mediated pathways. Front Immunol. 9(2049)2018.PubMed/NCBI View Article : Google Scholar

18 

Liu X, Cao H, Li J, Wang B, Zhang P, Zhang XD, Liu Z, Yuan H and Zhan Z: Autophagy induced by DAMPs facilitates the inflammation response in lungs undergoing ischemia-reperfusion injury through promoting TRAF6 ubiquitination. Cell Death Differ. 24:683–693. 2017.PubMed/NCBI View Article : Google Scholar

19 

He L, Wang Z, Zhou R, Xiong W, Yang Y, Song N and Qian J: Dexmedetomidine exerts cardioprotective effect through miR-146a-3p targeting IRAK1 and TRAF6 via inhibition of the NF-κB pathway. Biomed Pharmacother. 133(110993)2021.PubMed/NCBI View Article : Google Scholar

20 

Zhu Q, He G, Wang J, Wang Y and Chen W: Pretreatment with the ALDH2 agonist Alda-1 reduces intestinal injury induced by ischaemia and reperfusion in mice. Clin Sci (Lond). 131:1123–1136. 2017.PubMed/NCBI View Article : Google Scholar

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

22 

Ma Y, Guan Q, Bai A, Weiss CR, Hillman CL, Ma A, Zhou G, Qing G and Peng Z: Targeting TGF-beta1 by employing a vaccine ameliorates fibrosis in a mouse model of chronic colitis. Inflamm Bowel Dis. 16:1040–1050. 2010.PubMed/NCBI View Article : Google Scholar

23 

Liu J, Huang X, Hu S, He H and Meng Z: Dexmedetomidine attenuates lipopolysaccharide induced acute lung injury in rats by inhibition of caveolin-1 downstream signaling. Biomed Pharmacother. 118(109314)2019.PubMed/NCBI View Article : Google Scholar

24 

Kerzmann A, Haumann A, Boesmans E, Detry O and Defraigne JO: Acute mesenteric ischemia. Rev Med Liege. 73:300–303. 2018.PubMed/NCBI(In French).

25 

Yang Y, Yang F, Yu X, Wang B, Yang Y and Zhou X, Cheng R, Xia S and Zhou X: miR-16 inhibits NLRP3 inflammasome activation by directly targeting TLR4 in acute lung injury. Biomed Pharmacother. 112(108664)2019.PubMed/NCBI View Article : Google Scholar

26 

Kong F, Sun Y, Song W, Zhou Y and Zhu S: MiR-216a alleviates LPS-induced acute lung injury via regulating JAK2/STAT3 and NF-κB signaling. Hum Cell. 33:67–78. 2020.PubMed/NCBI View Article : Google Scholar

27 

He X, Zheng Y, Liu S, Shi S, Liu Y, He Y, Zhang C and Zhou X: MiR-146a protects small intestine against ischemia/reperfusion injury by down-regulating TLR4/TRAF6/NF-κB pathway. J Cell Physiol. 233:2476–2488. 2018.PubMed/NCBI View Article : Google Scholar

28 

Zhu Q, He G, Wang J, Wang Y and Chen W: Protective effects of fenofibrate against acute lung injury induced by intestinal ischemia/reperfusion in mice. Sci Rep. 6(22044)2016.PubMed/NCBI View Article : Google Scholar

29 

de Lima FM, Villaverde AB, Albertini R, Corrêa JC, Carvalho RLP, Munin E, Araújo T, Silva JA and Aimbire F: Dual Effect of low-level laser therapy (LLLT) on the acute lung inflammation induced by intestinal ischemia and reperfusion: Action on anti- and pro-inflammatory cytokines. Lasers Surg Med. 43:410–420. 2011.PubMed/NCBI View Article : Google Scholar

30 

Chen G, Zhang Z, Cheng Y, Xiao W, Qiu Y, Yu M, Sun L, Wang W, Du G, Gu Y, et al: The canonical Notch signaling was involved in the regulation of intestinal epithelial cells apoptosis after intestinal ischemia/reperfusion injury. Int J Mol Sci. 15:7883–7896. 2014.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Li G, Xu M, Wang H, Qi X, Wang X, Li Y, Sun J and Li Y: MicroRNA‑146a overexpression alleviates intestinal ischemia/reperfusion‑induced acute lung injury in mice. Exp Ther Med 22: 937, 2021.
APA
Li, G., Xu, M., Wang, H., Qi, X., Wang, X., Li, Y. ... Li, Y. (2021). MicroRNA‑146a overexpression alleviates intestinal ischemia/reperfusion‑induced acute lung injury in mice. Experimental and Therapeutic Medicine, 22, 937. https://doi.org/10.3892/etm.2021.10369
MLA
Li, G., Xu, M., Wang, H., Qi, X., Wang, X., Li, Y., Sun, J., Li, Y."MicroRNA‑146a overexpression alleviates intestinal ischemia/reperfusion‑induced acute lung injury in mice". Experimental and Therapeutic Medicine 22.3 (2021): 937.
Chicago
Li, G., Xu, M., Wang, H., Qi, X., Wang, X., Li, Y., Sun, J., Li, Y."MicroRNA‑146a overexpression alleviates intestinal ischemia/reperfusion‑induced acute lung injury in mice". Experimental and Therapeutic Medicine 22, no. 3 (2021): 937. https://doi.org/10.3892/etm.2021.10369
Copy and paste a formatted citation
x
Spandidos Publications style
Li G, Xu M, Wang H, Qi X, Wang X, Li Y, Sun J and Li Y: MicroRNA‑146a overexpression alleviates intestinal ischemia/reperfusion‑induced acute lung injury in mice. Exp Ther Med 22: 937, 2021.
APA
Li, G., Xu, M., Wang, H., Qi, X., Wang, X., Li, Y. ... Li, Y. (2021). MicroRNA‑146a overexpression alleviates intestinal ischemia/reperfusion‑induced acute lung injury in mice. Experimental and Therapeutic Medicine, 22, 937. https://doi.org/10.3892/etm.2021.10369
MLA
Li, G., Xu, M., Wang, H., Qi, X., Wang, X., Li, Y., Sun, J., Li, Y."MicroRNA‑146a overexpression alleviates intestinal ischemia/reperfusion‑induced acute lung injury in mice". Experimental and Therapeutic Medicine 22.3 (2021): 937.
Chicago
Li, G., Xu, M., Wang, H., Qi, X., Wang, X., Li, Y., Sun, J., Li, Y."MicroRNA‑146a overexpression alleviates intestinal ischemia/reperfusion‑induced acute lung injury in mice". Experimental and Therapeutic Medicine 22, no. 3 (2021): 937. https://doi.org/10.3892/etm.2021.10369
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