miR‑374a‑5p inhibits non‑small cell lung cancer cell proliferation and migration via targeting NCK1
- Qiang Guo
- Haijun Wang
- Yanzhao Xu
- Mingbo Wang
- Ziqiang Tian
Affiliations: Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of Thoracic Surgery, Xingtai People's Hospital, Xingtai, Hebei 054000, P.R. China
- Published online on: July 1, 2021 https://doi.org/10.3892/etm.2021.10375
Copyright: © Guo
et al. This is an open access article distributed under the
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Emerging studies have indicated that microRNAs (miRNAs/miRs) are involved in regulating non‑small cell lung cancer (NSCLC)‑associated processes. The present study aimed to evaluate the biological roles of miR‑374a‑5p in NSCLC. Using reverse transcription‑quantitative PCR, the expression levels of miR‑374a‑5p were determined in NSCLC cells and a normal cell line. Functional experiments were performed to investigate the functions of miR‑374a‑5p in NSCLC. A luciferase activity reporter assay and rescue experiments were performed to validate NCK adaptor protein 1 (NCK1) as a functional target of miR‑374a‑5p. It was demonstrated that miR‑374a‑5p levels were decreased in NSCLC cell lines compared with those in a normal cell line. Furthermore, overexpression of miR‑374a‑5p inhibited NSCLC cell proliferation and migration in vitro. Of note, NCK1 overexpression reversed the effects of miR‑375a‑5p on NSCLC cell proliferation and migration. The present results confirmed the tumor suppressor role of miR‑374a‑5p via targeting NCK1 in NSCLC, indicating the importance of the miR‑374a‑5p/NCK1 axis in NSCLC.