miR‑374a‑5p inhibits non‑small cell lung cancer cell proliferation and migration via targeting NCK1

Guo,Qiang; Wang,Haijun; Xu,Yanzhao; Wang,Mingbo; Tian,Ziqiang

doi:10.3892/etm.2021.10375

September-2021 Volume 22 Issue 3

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September-2021 Volume 22 Issue 3

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miR‑374a‑5p inhibits non‑small cell lung cancer cell proliferation and migration via targeting NCK1

Authors:
- Qiang Guo
- Haijun Wang
- Yanzhao Xu
- Mingbo Wang
- Ziqiang Tian
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Affiliations: Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of Thoracic Surgery, Xingtai People's Hospital, Xingtai, Hebei 054000, P.R. China

Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 943
|
Published online on: July 1, 2021

https://doi.org/10.3892/etm.2021.10375
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Abstract

Emerging studies have indicated that microRNAs (miRNAs/miRs) are involved in regulating non‑small cell lung cancer (NSCLC)‑associated processes. The present study aimed to evaluate the biological roles of miR‑374a‑5p in NSCLC. Using reverse transcription‑quantitative PCR, the expression levels of miR‑374a‑5p were determined in NSCLC cells and a normal cell line. Functional experiments were performed to investigate the functions of miR‑374a‑5p in NSCLC. A luciferase activity reporter assay and rescue experiments were performed to validate NCK adaptor protein 1 (NCK1) as a functional target of miR‑374a‑5p. It was demonstrated that miR‑374a‑5p levels were decreased in NSCLC cell lines compared with those in a normal cell line. Furthermore, overexpression of miR‑374a‑5p inhibited NSCLC cell proliferation and migration in vitro. Of note, NCK1 overexpression reversed the effects of miR‑375a‑5p on NSCLC cell proliferation and migration. The present results confirmed the tumor suppressor role of miR‑374a‑5p via targeting NCK1 in NSCLC, indicating the importance of the miR‑374a‑5p/NCK1 axis in NSCLC.

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1	Siegel RL, Miller KD and Jemal A: Cancer statistics, 2018. CA Cancer J Clin. 68:7–30. 2018.PubMed/NCBI View Article : Google Scholar
2	Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018.PubMed/NCBI View Article : Google Scholar
3	He L and Hannon GJ: MicroRNAs: Small RNAs with a big role in gene regulation. Nat Rev Genet. 5:522–531. 2004.PubMed/NCBI View Article : Google Scholar
4	Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004.PubMed/NCBI View Article : Google Scholar
5	Lages E, Ipas H, Guttin A, Nesr H, Berger F and Issartel JP: MicroRNAs: Molecular features and role in cancer. Front Biosci (Landmark Ed). 17:2508–2540. 2012.PubMed/NCBI View Article : Google Scholar
6	Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006.PubMed/NCBI View Article : Google Scholar
7	Lian F, Cui Y, Zhou C, Gao K and Wu L: Identification of a plasma four-microRNA panel as potential noninvasive biomarker for osteosarcoma. PLoS One. 10(e0121499)2015.PubMed/NCBI View Article : Google Scholar
8	Son D, Kim Y, Lim S, Kang HG, Kim DH, Park JW, Cheong W, Kong HK, Han W, Park WY, et al: miR-374a-5p promotes tumor progression by targeting ARRB1 in triple negative breast cancer. Cancer Lett. 454:224–233. 2019.PubMed/NCBI View Article : Google Scholar
9	Chen W, Zhang Y, Wang H, Pan T, Zhang Y and Li C: LINC00473/miR-374a-5p regulates esophageal squamous cell carcinoma via targeting SPIN1 to weaken the effect of radiotherapy. J Cell Biochem. 120:14562–14572. 2019.PubMed/NCBI View Article : Google Scholar
10	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar
11	Zhang B, Pan X, Cobb GP and Anderson TA: MicroRNAs as oncogenes and tumor suppressors. Dev Biol. 302:1–12. 2007.PubMed/NCBI View Article : Google Scholar
12	Li Y, Wang D, Li X, Shao Y, He Y, Yu H and Ma Z: MiR-199a-5p suppresses non-small cell lung cancer via targeting MAP3K11. J Cancer. 10:2472–2479. 2019.PubMed/NCBI View Article : Google Scholar
13	Yin G, Zhang B and Li J: miR-221-3p promotes the cell growth of non-small cell lung cancer by targeting p27. Mol Med Rep. 20:604–612. 2019.PubMed/NCBI View Article : Google Scholar
14	Liang H, Wang C, Gao K, Li J and Jia R: ΜicroRNA-421 promotes the progression of non-small cell lung cancer by targeting HOPX and regulating the Wnt/β-catenin signaling pathway. Mol Med Rep. 20:151–161. 2019.PubMed/NCBI View Article : Google Scholar
15	Zhang F, Lu YX, Chen Q, Zou HM, Zhang JM, Hu YH, Li XM, Zhang WJ, Zhang W, Lin C and Li XN: Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis. Cell Signal. 36:67–78. 2017.PubMed/NCBI View Article : Google Scholar
16	Xia P, Huang M, Zhang Y, Xiong X, Yan M, Xiong X, Yu W and Song E: NCK1 promotes the angiogenesis of cervical squamous carcinoma via Rac1/PAK1/MMP2 signal pathway. Gynecol Oncol. 152:387–395. 2019.PubMed/NCBI View Article : Google Scholar

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

miR‑374a‑5p inhibits non‑small cell lung cancer cell proliferation and migration via targeting NCK1

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Abstract

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