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Effects of intermittent fasting on liver physiology and metabolism in mice

  • Authors:
    • Jianbo Ma
    • Yan Cheng
    • Qiang Su
    • Wen Ai
    • Ling Gong
    • Yueying Wang
    • Linhao Li
    • Zhongren Ma
    • Qiuwei Pan
    • Zilin Qiao
    • Kan Chen
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, Gansu 730030, P.R. China, Department of Cardiology, Union Shenzhen Hospital, Huazhong University of Science and Technology, Shenzhen, Guangdong 518102, P.R. China, Department of Liver Diseases, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang 310015, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 950
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    Published online on: July 5, 2021
       https://doi.org/10.3892/etm.2021.10382
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Abstract

A broad spectrum of health benefits from intermittent fasting have been reported in studies on animal models and human subjects. However, the underlying mechanisms of these beneficial effects remain largely elusive. The present study aimed to explore the effects and potential mode of action of intermittent fasting in mouse models with a focus on the liver. C57BL/6 mice were subjected to intermittent fasting or ad libitum feeding as controls. It was determined that 12 h of daily intermittent fasting for 30 days significantly reduced the cumulative food intake compared with that in mice with ad libitum feeding. Fasting resulted in a significantly reduced liver mass but only had a minimal effect on bodyweight. The effects on the liver by 30 days of fasting were not reversed by subsequent ad libitum refeeding for 30 days. Among the measured blood biochemical parameters, the levels of blood glucose were decreased, while the levels of alkaline phosphatase were increased in fasting mice. Of note, targeted metabolic profiling revealed global elevation of metabolites in the livers of fasting mice. These metabolic molecules included adenosine triphosphate, nicotinamide adenine dinucleotide phosphate (NADP), reduced NADP and succinate, which are essentially involved in the citric acid cycle and oxidative phosphorylation. Thus, it was concluded that daily 12 h of intermittent fasting for one month significantly reduced the liver weight of mice, which is associated with enhanced liver metabolism.
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Copy and paste a formatted citation
Spandidos Publications style
Ma J, Cheng Y, Su Q, Ai W, Gong L, Wang Y, Li L, Ma Z, Pan Q, Qiao Z, Qiao Z, et al: Effects of intermittent fasting on liver physiology and metabolism in mice. Exp Ther Med 22: 950, 2021.
APA
Ma, J., Cheng, Y., Su, Q., Ai, W., Gong, L., Wang, Y. ... Chen, K. (2021). Effects of intermittent fasting on liver physiology and metabolism in mice. Experimental and Therapeutic Medicine, 22, 950. https://doi.org/10.3892/etm.2021.10382
MLA
Ma, J., Cheng, Y., Su, Q., Ai, W., Gong, L., Wang, Y., Li, L., Ma, Z., Pan, Q., Qiao, Z., Chen, K."Effects of intermittent fasting on liver physiology and metabolism in mice". Experimental and Therapeutic Medicine 22.3 (2021): 950.
Chicago
Ma, J., Cheng, Y., Su, Q., Ai, W., Gong, L., Wang, Y., Li, L., Ma, Z., Pan, Q., Qiao, Z., Chen, K."Effects of intermittent fasting on liver physiology and metabolism in mice". Experimental and Therapeutic Medicine 22, no. 3 (2021): 950. https://doi.org/10.3892/etm.2021.10382
Copy and paste a formatted citation
x
Spandidos Publications style
Ma J, Cheng Y, Su Q, Ai W, Gong L, Wang Y, Li L, Ma Z, Pan Q, Qiao Z, Qiao Z, et al: Effects of intermittent fasting on liver physiology and metabolism in mice. Exp Ther Med 22: 950, 2021.
APA
Ma, J., Cheng, Y., Su, Q., Ai, W., Gong, L., Wang, Y. ... Chen, K. (2021). Effects of intermittent fasting on liver physiology and metabolism in mice. Experimental and Therapeutic Medicine, 22, 950. https://doi.org/10.3892/etm.2021.10382
MLA
Ma, J., Cheng, Y., Su, Q., Ai, W., Gong, L., Wang, Y., Li, L., Ma, Z., Pan, Q., Qiao, Z., Chen, K."Effects of intermittent fasting on liver physiology and metabolism in mice". Experimental and Therapeutic Medicine 22.3 (2021): 950.
Chicago
Ma, J., Cheng, Y., Su, Q., Ai, W., Gong, L., Wang, Y., Li, L., Ma, Z., Pan, Q., Qiao, Z., Chen, K."Effects of intermittent fasting on liver physiology and metabolism in mice". Experimental and Therapeutic Medicine 22, no. 3 (2021): 950. https://doi.org/10.3892/etm.2021.10382
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