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Curcumin inhibits prostate cancer progression by regulating the miR‑30a‑5p/PCLAF axis

  • Authors:
    • Liang Pan
    • Jian Sha
    • Wenyao Lin
    • Yuxiong Wang
    • Tingzhang Bian
    • Jianming Guo
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Xuhui Hospital, Zhongshan Hospital Affiliated to Fudan University, Shanghai 200031, P.R. China, Department of Urology, Zhongshan Hospital Affiliated to Fudan University, Shanghai 200032, P.R. China
    Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 969
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    Published online on: July 7, 2021
       https://doi.org/10.3892/etm.2021.10401
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Abstract

Curcumin has been shown to inhibit the growth of a variety of tumor cells. However, the biological functions of curcumin in prostate cancer (PCa) have not yet fully elucidated. The objective of the present study was to investigate the role of curcumin on the proliferation, migration, invasion and apoptosis of PCa cells and the underlying mechanism. Cell Counting Kit‑8 and flow cytometry were used to detect the effects of curcumin at different concentrations on the proliferation and apoptosis of PCa cell lines, PC‑3 and DU145. BrdU and Transwell assays, western blotting and reverse transcription‑quantitative PCR were used to determine the effect of curcumin on cell proliferation, migration and invasion, apoptosis‑related protein expression, and microRNA (miR)‑30a‑5p and PCNA clamp associated factor (PCLAF) expression, respectively. In addition, bioinformatics analysis and Pearson's correlation test were used to verify the relationship between miR‑30a‑5p and PCLAF. Curcumin was observed to impede the proliferation, migration and invasion of PCa cells, and promote their apoptosis in a time‑ and dose‑dependent manner. Curcumin enhanced miR‑30a‑5p expression and inhibited PCLAF expression; furthermore, there was a negative correlation between miR‑30a‑5p and PCLAF expression in PCa tissues. In addition, transfection of miR‑30a‑5p inhibitors partially reversed the function of curcumin on cell proliferation, migration, invasion and apoptosis. Overall, curcumin suppressed the malignant biological behaviors of PCa cells by regulating the miR‑30a‑5p/PCLAF axis.
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Copy and paste a formatted citation
Spandidos Publications style
Pan L, Sha J, Lin W, Wang Y, Bian T and Guo J: Curcumin inhibits prostate cancer progression by regulating the miR‑30a‑5p/PCLAF axis. Exp Ther Med 22: 969, 2021.
APA
Pan, L., Sha, J., Lin, W., Wang, Y., Bian, T., & Guo, J. (2021). Curcumin inhibits prostate cancer progression by regulating the miR‑30a‑5p/PCLAF axis. Experimental and Therapeutic Medicine, 22, 969. https://doi.org/10.3892/etm.2021.10401
MLA
Pan, L., Sha, J., Lin, W., Wang, Y., Bian, T., Guo, J."Curcumin inhibits prostate cancer progression by regulating the miR‑30a‑5p/PCLAF axis". Experimental and Therapeutic Medicine 22.3 (2021): 969.
Chicago
Pan, L., Sha, J., Lin, W., Wang, Y., Bian, T., Guo, J."Curcumin inhibits prostate cancer progression by regulating the miR‑30a‑5p/PCLAF axis". Experimental and Therapeutic Medicine 22, no. 3 (2021): 969. https://doi.org/10.3892/etm.2021.10401
Copy and paste a formatted citation
x
Spandidos Publications style
Pan L, Sha J, Lin W, Wang Y, Bian T and Guo J: Curcumin inhibits prostate cancer progression by regulating the miR‑30a‑5p/PCLAF axis. Exp Ther Med 22: 969, 2021.
APA
Pan, L., Sha, J., Lin, W., Wang, Y., Bian, T., & Guo, J. (2021). Curcumin inhibits prostate cancer progression by regulating the miR‑30a‑5p/PCLAF axis. Experimental and Therapeutic Medicine, 22, 969. https://doi.org/10.3892/etm.2021.10401
MLA
Pan, L., Sha, J., Lin, W., Wang, Y., Bian, T., Guo, J."Curcumin inhibits prostate cancer progression by regulating the miR‑30a‑5p/PCLAF axis". Experimental and Therapeutic Medicine 22.3 (2021): 969.
Chicago
Pan, L., Sha, J., Lin, W., Wang, Y., Bian, T., Guo, J."Curcumin inhibits prostate cancer progression by regulating the miR‑30a‑5p/PCLAF axis". Experimental and Therapeutic Medicine 22, no. 3 (2021): 969. https://doi.org/10.3892/etm.2021.10401
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