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Expression of microRNA‑155‑5p in patients with refractory diabetic macular edema and its regulatory mechanism

  • Authors:
    • Junwen He
    • Rui Zhang
    • Shan Wang
    • Lu Xie
    • Chengfeng Yu
    • Tao Xu
    • Yanzi Li
    • Tao Yan
  • View Affiliations / Copyright

    Affiliations: Department of Retinal and Vitreous Diseases, Aier Eye Hospital of Wuhan University, Wuhan, Hubei 430000, P.R. China, Department of Ophthalmic Imaging, Aier Eye Hospital of Wuhan University, Wuhan, Hubei 430000, P.R. China
    Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 975
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    Published online on: July 8, 2021
       https://doi.org/10.3892/etm.2021.10407
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Abstract

Diabetic macular edema (DME) is the main cause of visual impairment in diabetic patients, but its pathogenesis remains unclear. The purpose of the present study was to analyze the expression of microRNA (miR)‑155‑5p in patients with DME and its regulatory mechanism. A total of 72 patients diagnosed with DME and 17 with idiopathic macular hole (MH) were recruited. Among samples from patients with DME, 45 were DME and 27 were refractory DME, whereas patients with idiopathic MH served as the control group. Optical coherence tomography and fundus photograph analysis revealed that part of the retina in the fundus of patients with DME was thickened, with macular edema occurring simultaneously. In refractory patients with DME, macular edema was associated with bleeding and a dark cavity between retinal layers. Through reverse transcription‑quantitative PCR analysis, miR‑155‑5p was highly expressed in the aqueous humor (AH) and plasma of patients with DME compared with that in patients with MH, and this was even higher in the refractory DME group. Upon analyzing patient clinical data, the difference in miR‑155‑5p expression in the AH and plasma was positively associated with disease course, body mass index, fasting blood‑glucose, glycated hemoglobin, proteinuria and glycosuria. The expression of miR‑155‑5p was not significantly different based on hemoglobin, intraocular pressure and sex. The aforementioned results indicate that miR‑155‑5p might promote the development of DME. To further study the molecular mechanism, human retinal microvascular endothelial cells (HRMECs) were cultured and treated with high glucose in vitro. The results showed that miR‑155‑5p expression was significantly upregulated in HRMECs induced by high glucose. After inhibiting the expression of miR‑155‑5p, cell proliferation, angiogenesis and VEGF protein levels were significantly downregulated, whereas miR‑155‑5p mimics had the opposite effect. In summary, miR‑155‑5p is closely associated with DME and is a potential target for refractory DME treatment.
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Copy and paste a formatted citation
Spandidos Publications style
He J, Zhang R, Wang S, Xie L, Yu C, Xu T, Li Y and Yan T: Expression of microRNA‑155‑5p in patients with refractory diabetic macular edema and its regulatory mechanism. Exp Ther Med 22: 975, 2021.
APA
He, J., Zhang, R., Wang, S., Xie, L., Yu, C., Xu, T. ... Yan, T. (2021). Expression of microRNA‑155‑5p in patients with refractory diabetic macular edema and its regulatory mechanism. Experimental and Therapeutic Medicine, 22, 975. https://doi.org/10.3892/etm.2021.10407
MLA
He, J., Zhang, R., Wang, S., Xie, L., Yu, C., Xu, T., Li, Y., Yan, T."Expression of microRNA‑155‑5p in patients with refractory diabetic macular edema and its regulatory mechanism". Experimental and Therapeutic Medicine 22.3 (2021): 975.
Chicago
He, J., Zhang, R., Wang, S., Xie, L., Yu, C., Xu, T., Li, Y., Yan, T."Expression of microRNA‑155‑5p in patients with refractory diabetic macular edema and its regulatory mechanism". Experimental and Therapeutic Medicine 22, no. 3 (2021): 975. https://doi.org/10.3892/etm.2021.10407
Copy and paste a formatted citation
x
Spandidos Publications style
He J, Zhang R, Wang S, Xie L, Yu C, Xu T, Li Y and Yan T: Expression of microRNA‑155‑5p in patients with refractory diabetic macular edema and its regulatory mechanism. Exp Ther Med 22: 975, 2021.
APA
He, J., Zhang, R., Wang, S., Xie, L., Yu, C., Xu, T. ... Yan, T. (2021). Expression of microRNA‑155‑5p in patients with refractory diabetic macular edema and its regulatory mechanism. Experimental and Therapeutic Medicine, 22, 975. https://doi.org/10.3892/etm.2021.10407
MLA
He, J., Zhang, R., Wang, S., Xie, L., Yu, C., Xu, T., Li, Y., Yan, T."Expression of microRNA‑155‑5p in patients with refractory diabetic macular edema and its regulatory mechanism". Experimental and Therapeutic Medicine 22.3 (2021): 975.
Chicago
He, J., Zhang, R., Wang, S., Xie, L., Yu, C., Xu, T., Li, Y., Yan, T."Expression of microRNA‑155‑5p in patients with refractory diabetic macular edema and its regulatory mechanism". Experimental and Therapeutic Medicine 22, no. 3 (2021): 975. https://doi.org/10.3892/etm.2021.10407
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