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DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation

  • Authors:
    • Yunchen Yu
    • Yuying Zhu
    • Xiaotong Sun
    • Yongxing Li
    • Mingling Wang
    • Bin Dong
    • Xiaodong Sun
    • Wenming Hou
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Surgery, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261000, P.R. China, School of Anesthesiology, Shandong Provincial Medicine and Health Key Laboratory of Clinical Anesthesia, Weifang Medical University, Weifang, Shandong 261000, P.R. China, Department of Endocrinology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261000, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1008
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    Published online on: July 15, 2021
       https://doi.org/10.3892/etm.2021.10441
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Abstract

DL‑3‑n‑butylphthalide (NBP) is commonly used to treat ischemic strokes due to its antioxidative and anti‑inflammatory effects. The present study aimed to examine the protective effects of NBP on myocardial ischemia‑reperfusion injury (MIRI) by establishing a MIRI model in H9c2 cells. Cell viability assay using Cell Counting Kit‑8, lactate dehydrogenase (LDH) cytotoxicity and lipid peroxidation malondialdehyde (MDA) content were assessed to detect cell activity, degree of cell injury and oxidative stress reaction. Reverse transcription‑quantitative PCR was used to quantify the expression of inflammatory factors in H9c2 cells. Western blotting and immunofluorescence staining were used to detect the protein expression of PI3K/AKT and heat shock protein 70 (HSP70). The present results indicated that NBP significantly increased cell viability during ischemia‑reperfusion. Moreover, NBP inhibited the release of LDH and the production of MDA. NBP treatment also significantly decreased the expression of inflammatory factors at the mRNA level. Additionally, NBP activated the PI3K/AKT pathway and upregulated the expression of HSP70 compared with cells in the MIRI model. LY294002, a PI3K inhibitor, reversed the protective effects of NBP and suppressed the expression of HSP70. The present study demonstrated that NBP protected H9c2 cells from MIRI by regulating HSP70 expression via PI3K/AKT pathway activation.
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1 

Wang BF and Yoshioka J: The emerging role of thioredoxin-interacting protein in myocardial ischemia/reperfusion injury. J Cardiovasc Pharmacol Ther. 22:219–229. 2017.PubMed/NCBI View Article : Google Scholar

2 

Li X, Liu M, Sun R, Zeng Y, Chen S and Zhang P: Protective approaches against myocardial ischemia reperfusion injury. Exp Ther Med. 12:3823–3829. 2016.PubMed/NCBI View Article : Google Scholar

3 

Chin KY, Qin C, May L, Ritchie RH and Woodman OL: New pharmacological approaches to the prevention of myocardial ischemia-reperfusion injury. Curr Drug Targets. 18:1689–1711. 2017.PubMed/NCBI View Article : Google Scholar

4 

Esposito ML, Zhang Y, Qiao X, Reyelt L, Paruchuri V, Schnitzler GR, Morine KJ, Annamalai SK, Bogins C, Natov PS, et al: Left ventricular unloading before reperfusion promotes functional recovery after acute myocardial infarction. J Am Coll Cardiol. 72:501–514. 2018.PubMed/NCBI View Article : Google Scholar

5 

Wang Z, Wang Y, Ye J, Lu X, Cheng Y, Xiang L, Chen L, Feng W, Shi H, Yu X, et al: bFGF attenuates endoplasmic reticulum stress and mitochondrial injury on myocardial ischaemia/reperfusion via activation of PI3K/Akt/ERK1/2 pathway. J Cell Mol Med. 19:595–607. 2015.PubMed/NCBI View Article : Google Scholar

6 

Radons J: The human HSP70 family of chaperones: Where do we stand? Cell Stress Chaperones. 21:379–404. 2016.PubMed/NCBI View Article : Google Scholar

7 

Guo S, Gao C, Xiao W, Zhang J, Qu Y, Li J and Ye F: Matrine protects cardiomyocytes from ischemia/reperfusion injury by regulating HSP70 expression via activation of the JAK2/STAT3 pathway. Shock. 50:664–670. 2018.PubMed/NCBI View Article : Google Scholar

8 

Wright MA, Aprile FA, Bellaiche MM, Michaels TC, Muller T, Arosio P, Vendruscolo M, Dobson CM and Knowles TP: Cooperative assembly of Hsp70 subdomain clusters. Biochemistry. 57:3641–3649. 2018.PubMed/NCBI View Article : Google Scholar

9 

Tang L, Mo Y, Li Y, Zhong Y, He S, Zhang Y, Tang Y, Fu S, Wang X and Chen A: Urolithin A alleviates myocardial ischemia/reperfusion injury via PI3K/Akt pathway. Biochem Biophys Res Commun. 486:774–780. 2017.PubMed/NCBI View Article : Google Scholar

10 

Zhang BF, Jiang H, Chen J, Guo X, Li Y, Hu Q and Yang S: Nobiletin ameliorates myocardial ischemia and reperfusion injury by attenuating endoplasmic reticulum stress-associated apoptosis through regulation of the PI3K/AKT signal pathway. Int Immunopharmacol. 73:98–107. 2019.PubMed/NCBI View Article : Google Scholar

11 

Thokala S, Inapurapu S, Bodiga VL, Vemuri PK and Bodiga S: Loss of ErbB2-PI3K/Akt signaling prevents zinc pyrithione-induced cardioprotection during ischemia/reperfusion. Biomed Pharmacother. 88:309–324. 2017.PubMed/NCBI View Article : Google Scholar

12 

Vanhaesebroeck B, Guillermet-Guibert J, Graupera M and Bilanges B: The emerging mechanisms of isoform-specific PI3K signalling. Nat Rev Mol Cell Biol. 11:329–341. 2010.PubMed/NCBI View Article : Google Scholar

13 

Sulaiman D, Li J, Devarajan A, Cunningham CM, Li M, Fishbein GA, Fogelman AM, Eghbali M and Reddy ST: Paraoxonase 2 protects against acute myocardial ischemia-reperfusion injury by modulating mitochondrial function and oxidative stress via the PI3K/Akt/GSK-3β RISK pathway. J Mol Cell Cardiol. 129:154–164. 2019.PubMed/NCBI View Article : Google Scholar

14 

Hausenloy DJ and Yellon DM: New directions for protecting the heart against ischaemia-reperfusion injury: Targeting the reperfusion injury salvage kinase (RISK)-pathway. Cardiovasc Res. 61:448–460. 2004.PubMed/NCBI View Article : Google Scholar

15 

Damilano F, Perino A and Hirsch E: PI3K kinase and scaffold functions in heart. Ann N Y Acad Sci. 1188:39–45. 2010.PubMed/NCBI View Article : Google Scholar

16 

Zhai C, Lv J, Wang K, Li Q and Qu Y: HSP70 silencing aggravates apoptosis induced by hypoxia/reoxygenation in vitro. Exp Ther Med. 18:1013–1020. 2019.PubMed/NCBI View Article : Google Scholar

17 

Ji K, Xue L, Cheng J and Bai Y: Preconditioning of H2S inhalation protects against cerebral ischemia/reperfusion injury by induction of HSP70 through PI3K/Akt/Nrf2 pathway. Brain Res Bull. 121:68–74. 2016.PubMed/NCBI View Article : Google Scholar

18 

Abdoulaye IA and Guo YJ: A review of recent advances in neuroprotective potential of 3-N-Butylphthalide and its derivatives. Biomed Res Int. 2016(5012341)2016.PubMed/NCBI View Article : Google Scholar

19 

Zhao Y, Lee JH, Chen D, Gu X, Caslin A, Li J, Yu SP and Wei L: DL-3-n-butylphthalide induced neuroprotection, regenerative repair, functional recovery and psychological benefits following traumatic brain injury in mice. Neurochem Int. 111:82–92. 2017.PubMed/NCBI View Article : Google Scholar

20 

Qin C, Zhou P, Wang L, Mamtilahun M, Li W, Zhang Z, Yang GY and Wang Y: Dl-3-N-butylphthalide attenuates ischemic reperfusion injury by improving the function of cerebral artery and circulation. J Cereb Blood Flow Metab. 39:2011–2021. 2019.PubMed/NCBI View Article : Google Scholar

21 

Kawasaki K, Yano K, Sasaki K, Tawara S, Ikegaki I, Satoh S, Ohtsuka Y, Yoshino Y, Kuriyama H, Asano T and Seto M: Correspondence between neurological deficit, cerebral infarct size, and rho-kinase activity in a rat cerebral thrombosis model. J Mol Neurosci. 39:59–68. 2009.PubMed/NCBI View Article : Google Scholar

22 

Zhao Q, Zhang C, Wang X, Chen L, Ji H and Zhang Y: (S)-ZJM-289, a nitric oxide-releasing derivative of 3-n-butylphthalide, protects against ischemic neuronal injury by attenuating mitochondrial dysfunction and associated cell death. Neurochem Int. 60:134–144. 2012.PubMed/NCBI View Article : Google Scholar

23 

Zhang P, Guo ZF, Xu YM, Li YS and Song JG: N-Butylphthalide (NBP) ameliorated cerebral ischemia reperfusion-induced brain injury via HGF-regulated TLR4/NF-κB signaling pathway. Biomed Pharmacother. 83:658–666. 2016.PubMed/NCBI View Article : Google Scholar

24 

Liao D, Xiang D, Dang R, Xu P, Wang J, Han W, Fu Y, Yao D, Cao L and Jiang P: Neuroprotective effects of dl-3-n-Butylphthalide against doxorubicin-induced neuroinflammation, oxidative stress, endoplasmic reticulum stress, and behavioral changes. Oxid Med Cell Longev. 2018(9125601)2018.PubMed/NCBI View Article : Google Scholar

25 

Wang YG, Li Y, Wang CY, Ai JW, Dong XY, Huang HY, Feng ZY, Pan YM, Lin Y, Wang BX and Yao LL: L-3-n-Butylphthalide protects rats' cardiomyocytes from ischaemia/reperfusion-induced apoptosis by affecting the mitochondrial apoptosis pathway. Acta Physiol (Oxf). 210:524–533. 2014.PubMed/NCBI View Article : Google Scholar

26 

Qiu H, Wu H, Ma J, Cao H, Huang L, Qiu W, Peng Y and Ding C: DL-3-n-Butylphthalide reduces atrial fibrillation susceptibility by inhibiting atrial structural remodeling in rats with heart failure. Naunyn Schmiedebergs Arch Pharmacol. 391:323–334. 2018.PubMed/NCBI View Article : Google Scholar

27 

Qiu H, Ma J, Wu H and Ding C: DL-3-n-butylphthalide improves ventricular function, and prevents ventricular remodeling and arrhythmias in post-MI rats. Naunyn Schmiedebergs Arch Pharmacol. 391:627–637. 2018.PubMed/NCBI View Article : Google Scholar

28 

Yu P, Ma S, Dai X and Cao F: Elabela alleviates myocardial ischemia reperfusion-induced apoptosis, fibrosis and mitochondrial dysfunction through PI3K/AKT signaling. Am J Transl Res. 12:4467–4477. 2020.PubMed/NCBI

29 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

30 

Zhao D, Yang J and Yang L: Insights for oxidative stress and mTOR signaling in myocardial ischemia/reperfusion injury under diabetes. Oxid Med Cell Longev. 2017(6437467)2017.PubMed/NCBI View Article : Google Scholar

31 

Amani M, Jeddi S, Ahmadiasl N, Usefzade N and Zaman J: Effect of HEMADO on level of CK-MB and LDH enzymes after ischemia/reperfusion injury in isolated rat heart. Bioimpacts. 3:101–104. 2013.PubMed/NCBI View Article : Google Scholar

32 

Xu Z, Wang D, Zhou Z, Chen Q, Zhang D, Chen S, Jiang H, Jia C and Liu X: Dexmedetomidine attenuates renal and myocardial ischemia/reperfusion injury in a dose-dependent manner by inhibiting inflammatory response. Ann Clin Lab Sci. 49:31–35. 2019.PubMed/NCBI

33 

Zhang C, He M, Ni L, He K, Su K, Deng Y, Li Y and Xia H: The role of arachidonic acid metabolism in myocardial ischemia-reperfusion injury. Cell Biochem Biophys. 78:255–265. 2020.PubMed/NCBI View Article : Google Scholar

34 

Liu H, Yang L, Wu HJ, Chen KH, Lin F, Li G, Sun HY, Xiao GS, Wang Y and Li GR: Water-soluble acacetin prodrug confers significant cardioprotection against ischemia/reperfusion injury. Sci Rep. 6(36435)2016.PubMed/NCBI View Article : Google Scholar

35 

Wang S, Ma F, Huang L, Zhang Y and Peng Y, Xing C, Feng Y, Wang X and Peng Y: Dl-3-n-Butylphthalide (NBP): A promising therapeutic agent for ischemic stroke. CNS Neurol Disord Drug Targets. 17:338–347. 2018.PubMed/NCBI View Article : Google Scholar

36 

Wang F, Ma J, Han F, Guo X, Meng L, Sun Y, Jin C, Duan H, Li H and Peng Y: DL-3-n-butylphthalide delays the onset and progression of diabetic cataract by inhibiting oxidative stress in rat diabetic model. Sci Rep. 6(19396)2016.PubMed/NCBI View Article : Google Scholar

37 

Bai M, Pan CL, Jiang GX, Zhang YM and Zhang Z: Effects of butylphthalide on oxidative stress and inflammatory response in rats with myocardial infarction through Akt/Nrf2 signaling pathway. Eur Rev Med Pharmacol Sci. 23:9642–9650. 2019.PubMed/NCBI View Article : Google Scholar

38 

Liu X, Zhang C, Zhang C, Li J, Guo W, Yan D, Yang C, Zhao J, Xia T, Wang Y, et al: Heat shock protein 70 inhibits cardiomyocyte necroptosis through repressing autophagy in myocardial ischemia/reperfusion injury. In Vitro Cell Dev Biol Anim. 52:690–698. 2016.PubMed/NCBI View Article : Google Scholar

39 

Yuan X, Juan Z, Zhang R, Sun X, Yan R, Yue F, Huang Y, Yu J and Xia X: Clemastine fumarate protects against myocardial ischemia reperfusion injury by activating the TLR4/PI3K/Akt signaling pathway. Front Pharmacol. 11(28)2020.PubMed/NCBI View Article : Google Scholar

40 

Tsikas D: Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges. Anal Biochem. 524:13–30. 2017.PubMed/NCBI View Article : Google Scholar

41 

Ma C, Xu Z and Lv H: Low n-6/n-3 PUFA ratio improves inflammation and myocardial ischemic reperfusion injury. Biochem Cell Biol. 97:621–629. 2019.PubMed/NCBI View Article : Google Scholar

42 

Xiong J, Yuan YJ, Xue FS, Wang Q, Cheng Y, Li RP, Liao X and Liu JH: Postconditioning with α7nAChR agonist attenuates systemic inflammatory response to myocardial ischemia-reperfusion injury in rats. Inflammation. 35:1357–1364. 2012.PubMed/NCBI View Article : Google Scholar

43 

Sun M, Dawood F, Wen WH, Chen M, Dixon I, Kirshenbaum LA and Liu PP: Excessive tumor necrosis factor activation after infarction contributes to susceptibility of myocardial rupture and left ventricular dysfunction. Circulation. 110:3221–3228. 2004.PubMed/NCBI View Article : Google Scholar

44 

Weber A, Wasiliew P and Kracht M: Interleukin-1 (IL-1) pathway. Sci Signal. 3(cm1)2010.PubMed/NCBI View Article : Google Scholar

45 

Neri M, Fineschi V, Di Paolo M, Pomara C, Riezzo I, Turillazzi E and Cerretani D: Cardiac oxidative stress and inflammatory cytokines response after myocardial infarction. Curr Vasc Pharmacol. 13:26–36. 2015.PubMed/NCBI View Article : Google Scholar

46 

Yao H and Han X and Han X: The cardioprotection of the insulin-mediated PI3K/Akt/mTOR signaling pathway. Am J Cardiovasc Drugs. 14:433–442. 2014.PubMed/NCBI View Article : Google Scholar

47 

Wei D, Xu H, Gai X and Jiang Y: Astragaloside IV alleviates myocardial ischemia-reperfusion injury in rats through regulating PI3K/AKT/GSK-3β signaling pathways. Acta Cir Bras. 34(e201900708)2019.PubMed/NCBI View Article : Google Scholar

48 

Juhaszova M, Zorov DB, Yaniv Y, Nuss HB, Wang S and Sollott SJ: Role of glycogen synthase kinase-3beta in cardioprotection. Circ Res. 104:1240–1252. 2009.PubMed/NCBI View Article : Google Scholar

49 

Son TW, Yun SP, Yong MS, Seo BN, Ryu JM, Youn HY, Oh YM and Han HJ: Netrin-1 protects hypoxia-induced mitochondrial apoptosis through HSP27 expression via DCC- and integrin a6b4-dependent Akt, GSK-3β, and HSF-1 in mesenchymal stem cells. Cell Death Dis. 4(e563)2013.PubMed/NCBI View Article : Google Scholar

50 

Nair SP and Sharma RK: Heat shock proteins and their expression in primary murine cardiac cell populations during ischemia and reperfusion. Mol Cell Biochem. 464:21–26. 2020.PubMed/NCBI View Article : Google Scholar

51 

Xu NW, Chen Y, Liu W, Chen YJ, Fan ZM, Liu M and Li LJ: Inhibition of JAK2/STAT3 signaling pathway suppresses proliferation of burkitt's lymphoma raji cells via cell cycle progression, apoptosis, and oxidative stress by modulating HSP70. Med Sci Monit. 24:6255–6263. 2018.PubMed/NCBI View Article : Google Scholar

52 

Staib JL, Quindry JC, French JP, Criswell DS and Powers SK: Increased temperature, not cardiac load, activates heat shock transcription factor 1 and heat shock protein 72 expression in the heart. Am J Physiol Regul Integr Comp Physiol. 292:R432–R439. 2007.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Yu Y, Zhu Y, Sun X, Li Y, Wang M, Dong B, Sun X and Hou W: DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation. Exp Ther Med 22: 1008, 2021.
APA
Yu, Y., Zhu, Y., Sun, X., Li, Y., Wang, M., Dong, B. ... Hou, W. (2021). DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation. Experimental and Therapeutic Medicine, 22, 1008. https://doi.org/10.3892/etm.2021.10441
MLA
Yu, Y., Zhu, Y., Sun, X., Li, Y., Wang, M., Dong, B., Sun, X., Hou, W."DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation". Experimental and Therapeutic Medicine 22.3 (2021): 1008.
Chicago
Yu, Y., Zhu, Y., Sun, X., Li, Y., Wang, M., Dong, B., Sun, X., Hou, W."DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation". Experimental and Therapeutic Medicine 22, no. 3 (2021): 1008. https://doi.org/10.3892/etm.2021.10441
Copy and paste a formatted citation
x
Spandidos Publications style
Yu Y, Zhu Y, Sun X, Li Y, Wang M, Dong B, Sun X and Hou W: DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation. Exp Ther Med 22: 1008, 2021.
APA
Yu, Y., Zhu, Y., Sun, X., Li, Y., Wang, M., Dong, B. ... Hou, W. (2021). DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation. Experimental and Therapeutic Medicine, 22, 1008. https://doi.org/10.3892/etm.2021.10441
MLA
Yu, Y., Zhu, Y., Sun, X., Li, Y., Wang, M., Dong, B., Sun, X., Hou, W."DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation". Experimental and Therapeutic Medicine 22.3 (2021): 1008.
Chicago
Yu, Y., Zhu, Y., Sun, X., Li, Y., Wang, M., Dong, B., Sun, X., Hou, W."DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation". Experimental and Therapeutic Medicine 22, no. 3 (2021): 1008. https://doi.org/10.3892/etm.2021.10441
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