Open Access

DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation

  • Authors:
    • Yunchen Yu
    • Yuying Zhu
    • Xiaotong Sun
    • Yongxing Li
    • Mingling Wang
    • Bin Dong
    • Xiaodong Sun
    • Wenming Hou
  • View Affiliations

  • Published online on: July 15, 2021     https://doi.org/10.3892/etm.2021.10441
  • Article Number: 1008
  • Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

DL‑3‑n‑butylphthalide (NBP) is commonly used to treat ischemic strokes due to its antioxidative and anti‑inflammatory effects. The present study aimed to examine the protective effects of NBP on myocardial ischemia‑reperfusion injury (MIRI) by establishing a MIRI model in H9c2 cells. Cell viability assay using Cell Counting Kit‑8, lactate dehydrogenase (LDH) cytotoxicity and lipid peroxidation malondialdehyde (MDA) content were assessed to detect cell activity, degree of cell injury and oxidative stress reaction. Reverse transcription‑quantitative PCR was used to quantify the expression of inflammatory factors in H9c2 cells. Western blotting and immunofluorescence staining were used to detect the protein expression of PI3K/AKT and heat shock protein 70 (HSP70). The present results indicated that NBP significantly increased cell viability during ischemia‑reperfusion. Moreover, NBP inhibited the release of LDH and the production of MDA. NBP treatment also significantly decreased the expression of inflammatory factors at the mRNA level. Additionally, NBP activated the PI3K/AKT pathway and upregulated the expression of HSP70 compared with cells in the MIRI model. LY294002, a PI3K inhibitor, reversed the protective effects of NBP and suppressed the expression of HSP70. The present study demonstrated that NBP protected H9c2 cells from MIRI by regulating HSP70 expression via PI3K/AKT pathway activation.

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September-2021
Volume 22 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yu Y, Zhu Y, Sun X, Li Y, Wang M, Dong B, Sun X and Hou W: DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation. Exp Ther Med 22: 1008, 2021
APA
Yu, Y., Zhu, Y., Sun, X., Li, Y., Wang, M., Dong, B. ... Hou, W. (2021). DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation. Experimental and Therapeutic Medicine, 22, 1008. https://doi.org/10.3892/etm.2021.10441
MLA
Yu, Y., Zhu, Y., Sun, X., Li, Y., Wang, M., Dong, B., Sun, X., Hou, W."DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation". Experimental and Therapeutic Medicine 22.3 (2021): 1008.
Chicago
Yu, Y., Zhu, Y., Sun, X., Li, Y., Wang, M., Dong, B., Sun, X., Hou, W."DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation". Experimental and Therapeutic Medicine 22, no. 3 (2021): 1008. https://doi.org/10.3892/etm.2021.10441