Open Access

MicroRNA‑449a inhibits cell proliferation and migration by regulating mutant p53 in MDA‑MB‑468 cells

  • Authors:
    • Guangcheng Huang
    • Xiaowu Zhong
    • Lihua Yao
    • Qiang Ma
    • Hebin Liao
    • Lei Xu
    • Jiang Zou
    • Ru Sun
    • Dongsheng Wang
    • Xiaolan Guo
  • View Affiliations

  • Published online on: July 15, 2021     https://doi.org/10.3892/etm.2021.10452
  • Article Number: 1020
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the role of microRNA (miR)‑449a in the proliferation, migration and apoptosis of MDA‑MB‑468 breast cancer cells and examine the association between miR‑449a and mutant p53 in these cells. Cell proliferation, migration and invasion were examined using a crystal violet staining assay, wound healing scratch assay and Transwell assay, respectively. The expression level of miR‑449a and p53 was detected by reverse transcription‑quantitative PCR or western blotting. The results indicated that knockdown of mutant p53 suppressed the proliferation and migration of MDA‑MB‑468 cells by inhibiting the PI3K/AKT/mTOR signaling pathway. In addition, miR‑449a suppressed proliferation and migration via downregulation of mutant p53 expression in MDA‑MB‑468 cells. Therefore, miR‑449a may function as a tumor suppressor by regulating p53 expression in breast cancer cells, which may have potential implications in the treatment of patients with triple‑negative breast cancer carrying mutant p53.

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September-2021
Volume 22 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Huang G, Zhong X, Yao L, Ma Q, Liao H, Xu L, Zou J, Sun R, Wang D, Guo X, Guo X, et al: MicroRNA‑449a inhibits cell proliferation and migration by regulating mutant p53 in MDA‑MB‑468 cells. Exp Ther Med 22: 1020, 2021
APA
Huang, G., Zhong, X., Yao, L., Ma, Q., Liao, H., Xu, L. ... Guo, X. (2021). MicroRNA‑449a inhibits cell proliferation and migration by regulating mutant p53 in MDA‑MB‑468 cells. Experimental and Therapeutic Medicine, 22, 1020. https://doi.org/10.3892/etm.2021.10452
MLA
Huang, G., Zhong, X., Yao, L., Ma, Q., Liao, H., Xu, L., Zou, J., Sun, R., Wang, D., Guo, X."MicroRNA‑449a inhibits cell proliferation and migration by regulating mutant p53 in MDA‑MB‑468 cells". Experimental and Therapeutic Medicine 22.3 (2021): 1020.
Chicago
Huang, G., Zhong, X., Yao, L., Ma, Q., Liao, H., Xu, L., Zou, J., Sun, R., Wang, D., Guo, X."MicroRNA‑449a inhibits cell proliferation and migration by regulating mutant p53 in MDA‑MB‑468 cells". Experimental and Therapeutic Medicine 22, no. 3 (2021): 1020. https://doi.org/10.3892/etm.2021.10452