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CGFe and TGF‑β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway

  • Authors:
    • Xiaoju Li
    • Huixiao Yang
    • Yan Zhang
    • Xinya Du
    • Zhengbin Yan
    • Jiang Li
    • Bin Wu
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, The People's Hospital of Longhua, Shenzhen, Guangdong 518109, P.R. China, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatological Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China, Department of General Therapy Dentistry, Stomatology Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1048
    |
    Published online on: July 23, 2021
       https://doi.org/10.3892/etm.2021.10482
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Abstract

The present study aimed to evaluate the effects of concentrated growth factor exudate (CGFe) and TGF‑β1 on the viability and osteogenic differentiation of human dental pulp stem cells (hDPSCs). CGFe was prepared from the peripheral blood of healthy donors (obtained with informed consent). STRO‑1+ hDPSCs were isolated from dental pulp tissues and treated in four groups: i) Control; ii) TGF‑β1 (1 ng/ml); iii) 100% CGFe; and iv) TGF‑β1 (1 ng/ml) + 100% CGFe group. hDPSC viability was measured via MTT assay. The osteogenic differentiation of hDPSCs was quantified via alkaline phosphatase (ALP) activity, western blotting and reverse transcription‑quantitative PCR assays. CGFe and TGF‑β1 enhanced hDPSC viability, upregulated ALP activity, upregulated the expression of phosphorylated (p)‑ERK1/2, p‑JNK and p‑p38 in hDPSCs, and promoted transcription and protein expression of osteogenic‑related genes (bone sialoprotein, Runt‑related transcription factor 2 and osteocalcin) in hDPSCs. The present study demonstrated that CGFe and TGF‑β1 facilitated the viability and osteogenic differentiation of hDPSCs potentially through activation of the MAPK signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Yang H, Zhang Y, Du X, Yan Z, Li J and Wu B: CGFe and TGF‑β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway. Exp Ther Med 22: 1048, 2021.
APA
Li, X., Yang, H., Zhang, Y., Du, X., Yan, Z., Li, J., & Wu, B. (2021). CGFe and TGF‑β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway. Experimental and Therapeutic Medicine, 22, 1048. https://doi.org/10.3892/etm.2021.10482
MLA
Li, X., Yang, H., Zhang, Y., Du, X., Yan, Z., Li, J., Wu, B."CGFe and TGF‑β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway". Experimental and Therapeutic Medicine 22.4 (2021): 1048.
Chicago
Li, X., Yang, H., Zhang, Y., Du, X., Yan, Z., Li, J., Wu, B."CGFe and TGF‑β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1048. https://doi.org/10.3892/etm.2021.10482
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Yang H, Zhang Y, Du X, Yan Z, Li J and Wu B: CGFe and TGF‑β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway. Exp Ther Med 22: 1048, 2021.
APA
Li, X., Yang, H., Zhang, Y., Du, X., Yan, Z., Li, J., & Wu, B. (2021). CGFe and TGF‑β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway. Experimental and Therapeutic Medicine, 22, 1048. https://doi.org/10.3892/etm.2021.10482
MLA
Li, X., Yang, H., Zhang, Y., Du, X., Yan, Z., Li, J., Wu, B."CGFe and TGF‑β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway". Experimental and Therapeutic Medicine 22.4 (2021): 1048.
Chicago
Li, X., Yang, H., Zhang, Y., Du, X., Yan, Z., Li, J., Wu, B."CGFe and TGF‑β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1048. https://doi.org/10.3892/etm.2021.10482
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