Open Access

Network pharmacology‑based prediction of the active compounds and mechanism of Buyang Huanwu Decoction for ischemic stroke

  • Authors:
    • Kai Wang
    • Lu Lei
    • Jinyi Cao
    • Yi Qiao
    • Ruimin Liang
    • Jialin Duan
    • Zhijun Feng
    • Yi Ding
    • Yang Ma
    • Zhifu Yang
    • Enhu Zhang
  • View Affiliations

  • Published online on: July 23, 2021     https://doi.org/10.3892/etm.2021.10484
  • Article Number: 1050
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Buyang Huanwu Decoction (BYHWD) is used to promote blood circulation and is widely used in Chinese clinical practice for the treatment and prevention of ischemic cerebral vascular diseases. However, the mechanism and active compounds of BYHWD used to treat ischemic stroke are not well understood. The current study aimed to identify the potential active components of BYHWD and explore its mechanism using network pharmacology and bioinformatics analyses. The compounds of BYHWD were obtained from public databases. Oral bioavailability and drug‑likeness were screened using the absorption, distribution, metabolism and excretion (ADME) criteria. Components of BYHWD, alongside the candidate targets of each component and the known therapeutic targets of ischemic stroke were collected. A network of target gene compounds and cerebral ischemia compounds was established using network pharmacology data sources. The enrichment of key targets and pathways was analyzed using STRING and DAVID databases. Moreover, three of key targets [IL6, VEGFA and hypoxia‑inducible‑factor‑1α (HIF‑1α)] were verified using western blot analysis. Network analysis determined 102 compounds in seven herbal medicines that were subjected to ADME screening. A total of 42 compounds as well as 79 genes formed the principal pathways associated with ischemic stroke. The 16 key compounds identified were baicalein, beta‑carotene, baicalin, kaempferol, luteolin, quercetin, hydroxysafflor yellow A, isorhamnetin, bifendate, formononetin, calycosin, astragaloside IV, stigmasterol, sitosterol, Z‑ligustilide, and dihydrocapsaicin. The core genes in this network were IL6, TNF, VEGFA, HIF‑1α, MAPK1, MAPK3, JUN, STAT3, IL1B and IL10. Furthermore, the TNF, IL17, apoptosis, PI3K‑Akt, toll‑like receptor, MAPK, NF‑κB and HIF‑1 signaling pathways were identified to be associated with ischemic stroke. Compared with the control group (no treatment), BYHWD significantly inhibited the expression of IL6 and increase the expression of HIF‑1α and VEGFA. Network pharmacology analyses can help to reveal close interactions between multi‑components and multi‑targets and enhance understanding of the potential effects of BYHWD on ischemic stroke.
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October-2021
Volume 22 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Wang K, Lei L, Cao J, Qiao Y, Liang R, Duan J, Feng Z, Ding Y, Ma Y, Yang Z, Yang Z, et al: Network pharmacology‑based prediction of the active compounds and mechanism of Buyang Huanwu Decoction for ischemic stroke. Exp Ther Med 22: 1050, 2021
APA
Wang, K., Lei, L., Cao, J., Qiao, Y., Liang, R., Duan, J. ... Zhang, E. (2021). Network pharmacology‑based prediction of the active compounds and mechanism of Buyang Huanwu Decoction for ischemic stroke. Experimental and Therapeutic Medicine, 22, 1050. https://doi.org/10.3892/etm.2021.10484
MLA
Wang, K., Lei, L., Cao, J., Qiao, Y., Liang, R., Duan, J., Feng, Z., Ding, Y., Ma, Y., Yang, Z., Zhang, E."Network pharmacology‑based prediction of the active compounds and mechanism of Buyang Huanwu Decoction for ischemic stroke". Experimental and Therapeutic Medicine 22.4 (2021): 1050.
Chicago
Wang, K., Lei, L., Cao, J., Qiao, Y., Liang, R., Duan, J., Feng, Z., Ding, Y., Ma, Y., Yang, Z., Zhang, E."Network pharmacology‑based prediction of the active compounds and mechanism of Buyang Huanwu Decoction for ischemic stroke". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1050. https://doi.org/10.3892/etm.2021.10484