Inhibition of autophagy promotes human RSV NS1‑induced inflammation and apoptosis <em>in vitro</em>

Han,Bing; Wang,Yizhong; Zheng,Mei

doi:10.3892/etm.2021.10488

Inhibition of autophagy promotes human RSV NS1‑induced inflammation and apoptosis in vitro

Authors:
- Bing Han
- Yizhong Wang
- Mei Zheng
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Affiliations: Department of Pediatrics, Xiangyang No. 1 People's Hospital, Hubei University of Medicine, Xiangyang, Hubei 441000, P.R. China
Published online on: July 23, 2021 https://doi.org/10.3892/etm.2021.10488
Article Number: 1054
Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Human respiratory syncytial virus (RSV) is a major health challenge due to the lack of a safe and effective vaccine and antiviral drugs. RSV non‑structural protein 1 (NS1) is the main inhibitor of antiviral signaling pathways in RSV infection; however, the underlying mechanism is unclear. The aim of the present study was to investigate of the role of NS1 and its relationship with autophagy. NS1‑Flag plasmid was transfected into A549 cells and the levels of inflammatory cytokines, autophagy markers and apoptosis were detected. In addition, the cells were treated with an autophagy inhibitor, 3‑methyladenine for 12 h prior to transfection with the NS1 plasmid to explore the role of autophagy in NS1‑transfected cells. The results showed that the production of inflammatory cytokines and autophagy was induced in NS1‑transfected cells, and indicated that autophagy prevents the production of cytokines and the activation of apoptosis. Furthermore, the results demonstrated that NS1 activated autophagy partly through the mTOR‑p70 S6 kinase signaling pathway. The results suggest that autophagy induced by NS1 transfection through the mTOR pathway can hinder the production of inflammatory cytokines and interferon‑α and inhibit cell apoptosis, which may help to explain why autophagy has been shown to be beneficial to viral replication in most studies.

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