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Effect of the combination of astragaloside IV and Panax notoginseng saponins on pyroptosis and necroptosis in rat models of cerebral ischemia‑reperfusion

  • Authors:
    • Biao Tang
    • Xu She
    • Chang-Qing Deng
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, Medical School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, P.R. China
    Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1123
    |
    Published online on: August 4, 2021
       https://doi.org/10.3892/etm.2021.10557
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Abstract

Pyroptosis and necroptosis are closely associated with the mechanism underlying cerebral ischemia‑reperfusion (I/R) injury. The combination of astragaloside IV (AST IV) and Panax notoginseng saponins (PNS) has remarkable effects on the alleviation of cerebral I/R damage. However, whether inhibition of pyroptosis and necroptosis is the mechanism underlying the beneficial effects of this drug combination on cerebral I/R injury remains unclear. To explore the effects and mechanisms of drug treatment, middle cerebral artery occlusion was performed to induce I/R injury in rats, which was verified based on neurological deficit score (NDS), infarct volume and H&E staining. Activation of pyroptosis and necroptosis was detected by western blot analysis of associated proteins. The results of the present study demonstrated that treatment with AST IV and PNS, either alone or in combination, significantly reduced the NDS, cerebral infarct volume and cell injury rate in the cerebral cortex of rats. The treatments also improved pathological injury to the cerebral cortex and reduced the levels of proteins associated with pyroptosis and necroptosis. These effects were stronger in the combination drug group compared with groups treated with a single drug alone. The findings of the present study suggested that the combination of AST IV and PNS exhibited stronger neuroprotective effects in I/R injury than either drug alone, and that the underlying mechanism was associated with inhibition of pyroptosis and necroptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Tang B, She X and Deng C: Effect of the combination of astragaloside IV and <em>Panax</em> <em>notoginseng</em> saponins on pyroptosis and necroptosis in rat models of cerebral ischemia‑reperfusion. Exp Ther Med 22: 1123, 2021.
APA
Tang, B., She, X., & Deng, C. (2021). Effect of the combination of astragaloside IV and <em>Panax</em> <em>notoginseng</em> saponins on pyroptosis and necroptosis in rat models of cerebral ischemia‑reperfusion. Experimental and Therapeutic Medicine, 22, 1123. https://doi.org/10.3892/etm.2021.10557
MLA
Tang, B., She, X., Deng, C."Effect of the combination of astragaloside IV and <em>Panax</em> <em>notoginseng</em> saponins on pyroptosis and necroptosis in rat models of cerebral ischemia‑reperfusion". Experimental and Therapeutic Medicine 22.4 (2021): 1123.
Chicago
Tang, B., She, X., Deng, C."Effect of the combination of astragaloside IV and <em>Panax</em> <em>notoginseng</em> saponins on pyroptosis and necroptosis in rat models of cerebral ischemia‑reperfusion". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1123. https://doi.org/10.3892/etm.2021.10557
Copy and paste a formatted citation
x
Spandidos Publications style
Tang B, She X and Deng C: Effect of the combination of astragaloside IV and <em>Panax</em> <em>notoginseng</em> saponins on pyroptosis and necroptosis in rat models of cerebral ischemia‑reperfusion. Exp Ther Med 22: 1123, 2021.
APA
Tang, B., She, X., & Deng, C. (2021). Effect of the combination of astragaloside IV and <em>Panax</em> <em>notoginseng</em> saponins on pyroptosis and necroptosis in rat models of cerebral ischemia‑reperfusion. Experimental and Therapeutic Medicine, 22, 1123. https://doi.org/10.3892/etm.2021.10557
MLA
Tang, B., She, X., Deng, C."Effect of the combination of astragaloside IV and <em>Panax</em> <em>notoginseng</em> saponins on pyroptosis and necroptosis in rat models of cerebral ischemia‑reperfusion". Experimental and Therapeutic Medicine 22.4 (2021): 1123.
Chicago
Tang, B., She, X., Deng, C."Effect of the combination of astragaloside IV and <em>Panax</em> <em>notoginseng</em> saponins on pyroptosis and necroptosis in rat models of cerebral ischemia‑reperfusion". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1123. https://doi.org/10.3892/etm.2021.10557
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