Open Access

lncRNA MEG8 is downregulated in osteoarthritis and regulates chondrocyte cell proliferation, apoptosis and inflammation

  • Authors:
    • Wei Xie
    • Luoyong Jiang
    • Xiaoyang Huang
    • Hongxi Shang
    • Minghong Gao
    • Wei You
    • Jifeng Tan
    • Hong Yan
    • Wei Sun
  • View Affiliations

  • Published online on: August 10, 2021     https://doi.org/10.3892/etm.2021.10587
  • Article Number: 1153
  • Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Long noncoding RNA (lncRNA) maternally expressed 8, small nucleolar RNA host gene (MEG8) has been widely reported for its pro‑proliferative, anti‑apoptotic and anti‑inflammatory effects in diverse diseases. The aim of the present study was to investigate the effects and underlying mechanism of MEG8 on IL‑1β‑stimulated human osteoarthritis (OA) chondrocytes. C28/I2 chondrocytes were cultured under the stimulation of IL‑1β to establish a cellular model of OA. Functional assays involving Cell Counting Kit‑8 and flow cytometry were performed to determine proliferation and apoptosis in the cells. The protein expression levels of caspase‑3 and inflammatory cytokines were detected using cell‑based ELISA. The expression levels of PI3K/AKT pathway‑related proteins were evaluated by western blotting. It was identified that MEG8 expression was increased in the cartilage of patients with OA and in IL‑1β‑treated C28/I2 cells. In C28/I2 cells, silencing of MEG8 expression noticeably triggered IL‑1β‑induced proliferation suppression, cell death and an inflammatory response. However, transfection with MEG8 displayed adverse effects. Furthermore, MEG8 overexpression prevented IL‑1β‑induced activation of the PI3K/AKT signaling pathway in C28/I2 cells. These data demonstrated that MEG8 exerted protective effects against IL‑1β‑induced apoptosis and inflammation of OA chondrocytes by regulating the PI3K/AKT signaling pathway. Thus, the present study demonstrates that MEG8 might be a promising target for the treatment of OA.
View Figures
View References

Related Articles

Journal Cover

October-2021
Volume 22 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Xie W, Jiang L, Huang X, Shang H, Gao M, You W, Tan J, Yan H and Sun W: lncRNA MEG8 is downregulated in osteoarthritis and regulates chondrocyte cell proliferation, apoptosis and inflammation. Exp Ther Med 22: 1153, 2021
APA
Xie, W., Jiang, L., Huang, X., Shang, H., Gao, M., You, W. ... Sun, W. (2021). lncRNA MEG8 is downregulated in osteoarthritis and regulates chondrocyte cell proliferation, apoptosis and inflammation. Experimental and Therapeutic Medicine, 22, 1153. https://doi.org/10.3892/etm.2021.10587
MLA
Xie, W., Jiang, L., Huang, X., Shang, H., Gao, M., You, W., Tan, J., Yan, H., Sun, W."lncRNA MEG8 is downregulated in osteoarthritis and regulates chondrocyte cell proliferation, apoptosis and inflammation". Experimental and Therapeutic Medicine 22.4 (2021): 1153.
Chicago
Xie, W., Jiang, L., Huang, X., Shang, H., Gao, M., You, W., Tan, J., Yan, H., Sun, W."lncRNA MEG8 is downregulated in osteoarthritis and regulates chondrocyte cell proliferation, apoptosis and inflammation". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1153. https://doi.org/10.3892/etm.2021.10587