Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
October-2021 Volume 22 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2021 Volume 22 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

miR‑184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1

  • Authors:
    • Gui-Geng Tan
    • Chang Xu
    • Wei-Kang Zhong
    • Chuan-Yun Wang
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Affiliated Hospital of Jining Medical University, Jining, Shandong 272100, P.R. China, Department of Urology, Yanzhou People's Hospital, Jining, Shandong 272100, P.R. China, Operating Room Department, Affiliated Hospital of Jining Medical University, Jining, Shandong 272100, P.R. China, Department of Urinary Surgery, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China
    Copyright: © Tan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1163
    |
    Published online on: August 11, 2021
       https://doi.org/10.3892/etm.2021.10597
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

A number of previous studies have reported that dysregulated miR‑184 expression is associated with the development of cancer. The aim of the present study was to investigate the role of miR‑184 in prostate cancer (PC) and the mechanism underlying its effects. Data from human tumor tissue samples were collected from The CEancer Genome Atlas to determine the expression levels of miR‑184 and DLX1. The miR‑184 mimic and pcDNA3.1‑DLX1 plasmid were utilized to induce overexpression of miR‑184 and DLX1 in Du145 cells, respectively. Cell Counting Kit‑8, wound healing and Transwell assays were performed to examine the effects of miR‑184 on the aggressiveness of PC cells. Dual‑luciferase reporter gene assay was used to investigate the association between miR‑184 and DLX1, and reverse transcription‑quantitative PCR and western blot analyses were utilized to determine the mRNA and protein levels. miR‑184 expression was found to be downregulated whereas DLX1 was upregulated in PC tissues compared with normal prostate tissues. Cell propagation, migration and invasion were all inhibited by miR‑184 upregulation in Du145 cells. Dual luciferase reporter assay confirmed the association between miR‑184 and DLX1. The inhibitory effect of miR‑184 mimic on cell behaviors was reversed by upregulation of DLX1. These findings suggest that miR‑184 plays a beneficial role in suppressing the tumorigenesis of PC by directly targeting DLX1, and it may represent a potential therapeutic strategy for PC.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Cancer Stat Facts: Prostate Cancer in 2018; Available from: https://seer.cancer.gov/statfacts/html/prost.html.

2 

World Cancer Report 2014. World Health Organization. 2014. pp. Chapter 5.11.

3 

Caini S, Gandini S, Dudas M, Bremer V, Severi E and Gherasim A: Sexually transmitted infections and prostate cancer risk: A systematic review and meta-analysis. Cancer Epidemiol. 38:329–338. 2014.PubMed/NCBI View Article : Google Scholar

4 

Miller DC, Hafez KS, Stewart A, Montie JE and Wei JT: Prostate carcinoma presentation, diagnosis, and staging: An update form the national cancer data base. Cancer. 98:1169–1178. 2003.PubMed/NCBI View Article : Google Scholar

5 

Redman JM, Gulley JL and Madan RA: Combining immunotherapies for the treatment of prostate cancer. Urol Oncol. 35:694–700. 2017.PubMed/NCBI View Article : Google Scholar

6 

Jang TL, Kim IY, Scardino PT and Eastham JA: Reply to effectiveness of radical prostatectomy with adjuvant radiotherapy versus radiotherapy plus androgen deprivation therapy for men with advanced prostate cancer: Do we have certainties today? Cancer. 125:2318–2320. 2019.PubMed/NCBI View Article : Google Scholar

7 

Iorio MV and Croce CM: MicroRNA dysregulation in cancer: Diagnostics, monitoring and therapeutics. A comprehensive review. EMBO Mol Med. 4:143–159. 2012.PubMed/NCBI View Article : Google Scholar

8 

Pillai RS, Bhattacharyya SN and Filipowicz W: Repression of protein synthesis by miRNAs: How many mechanisms? Trends Cell Biol. 17:118–126. 2007.PubMed/NCBI View Article : Google Scholar

9 

O'Hara SP, Mott JL, Splinter PL, Gores GJ and LaRusso NF: MicroRNAs: Key modulators of posttranscriptional gene expression. Gastroenterology. 136:17–25. 2009.PubMed/NCBI View Article : Google Scholar

10 

Wang XJ, Reyes JL, Chua NH and Gaasterland T: Prediction and identification of arabidopsis thaliana microRNAs and their mRNA targets. Genome Biol. 5(R65)2004.PubMed/NCBI View Article : Google Scholar

11 

He J, Sun M, Geng H and Tian S: Long non-coding RNA Linc00518 promotes paclitaxel resistance of the human prostate cancer by sequestering miR-216b-5p. Biol Cell. 111:39–50. 2019.PubMed/NCBI View Article : Google Scholar

12 

Liang YK, Han ZD, Lu JM, Liu ZZ, Zhuo YJ, Zhu XJ, Chen JX, Ye JH, Liang YX, He HC and Zhong WD: Downregulation of ARID4A and ARID4B promote tumor progression and directly regulated by microRNA-30d in patient with prostate cancer. J Cell Biochem. 119:7245–7255. 2018.PubMed/NCBI View Article : Google Scholar

13 

Jiang W, Gao Y, Wang Z, Gong C, Hu C, Ding X, Qiang L, Gao S and Ren F: Co-delivery of miR-4638-5p and docetaxel based on redox-sensitive polypeptide micelles as an improved strategy for the treatment of castration-resistant prostate cancer. Mol Pharm. 16:437–447. 2019.PubMed/NCBI View Article : Google Scholar

14 

Khorasani M, Teimoori-Toolabi L, Farivar TN, Asgari M, Abolhasani M, Shahrokh H, Afgar A, Kalantari E, Peymani A and Mahdian R: Aberrant expression of miR-141 and nuclear receptor small heterodimer partner in clinical samples of prostate cancer. Cancer Biomark. 22:19–28. 2018.PubMed/NCBI View Article : Google Scholar

15 

Aboobaker AA, Tomancak P, Patel N, Rubin GM and Lai EC: Drosophila microRNAs exhibit diverse spatial expression patterns during embryonic development. Proc Natl Acad Sci USA. 102:18017–18022. 2005.PubMed/NCBI View Article : Google Scholar

16 

Wu X, Ding X, Ding Z and Jia P: Total flavonoids from leaves of carya cathayensis ameliorate renal fibrosis via the miR-21/Smad7 signaling pathway. Cell Physiol Biochem. 49:1551–1563. 2018.PubMed/NCBI View Article : Google Scholar

17 

Wu G, Liu J, Wu Z, Wu X and Yao X: MicroRNA-184 inhibits cell proliferation and metastasis in human colorectal cancer by directly targeting IGF-1R. Oncol Lett. 14:3215–3222. 2017.PubMed/NCBI View Article : Google Scholar

18 

Fang Z, Zhao J, Xie W, Sun Q, Wang H and Qiao B: LncRNA UCA1 promotes proliferation and cisplatin resistance of oral squamous cell carcinoma by suppressing miR-184 expression. Cancer Med. 6:2897–2908. 2017.PubMed/NCBI View Article : Google Scholar

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

20 

Qadir M and Faheem A: mRNA: A diagnostic and therapeutic tool for pancreatic cnacer. Crit Rev Eukaryot Gene Expr. 27:197–204. 2017.PubMed/NCBI View Article : Google Scholar

21 

Liang M, Sun Y, Yang HL, Zhang B, Wen J and Shi BK: DLX1, a binding protein of beta-catenin, promoted the growth and migration of prostate cancer cells. Exp Cell Res. 363:26–32. 2018.PubMed/NCBI View Article : Google Scholar

22 

Li JY, Wei X, Sun Q, Zhao XQ, Zheng CY, Bai CX, Du J, Zhang Z, Zhu LG and Jia YS: MicroRNA-449b-5p promotes the progression of osteoporosis by inhibiting osteogenic differentiation of BMSCs via targeting Satb2. Eur Rev Med Pharmacol Sci. 23:6394–6403. 2019.PubMed/NCBI View Article : Google Scholar

23 

Gironella M, Seux M, Xie MJ, Cano C, Tomasini R, Gommeaux J, Garcia S, Nowak J, Yeung ML, Jeang KT, et al: Tumor protein 53-induced nuclear protein 1 expression is repressed by miR-155, and its restoration inhibits pancreatic tumor development. Proc Natl Acad Sci USA. 104:16170–16175. 2007.PubMed/NCBI View Article : Google Scholar

24 

Akao Y, Nakagawa Y and Naoe T: MicroRNAs 143 and 145 are possible common onco-microRNAs in human cancers. Oncol Rep. 16:845–850. 2006.PubMed/NCBI

25 

Neilson JR, Zheng GX, Burge CB and Sharp PA: Dynamic regulation of miRNA expression in ordered stages of cellular development. Genes Dev. 21:578–589. 2007.PubMed/NCBI View Article : Google Scholar

26 

Chen XM, Splinter PL, O'Hara SP and LaRusso NF: A cellular micro-RNA, let-7i, regulates Toll-like receptor 4 expression and contributes to cholangiocyte immune responses against cryptosporidium parvum infection. J Biol Chem. 282:28929–28938. 2007.PubMed/NCBI View Article : Google Scholar

27 

Su Z, Chen D, Li Y, Zhang E, Yu Z, Chen T, Jiang Z, Ni L, Yang S, Gui Y, et al: microRNA-184 functions as tumor suppressor in renal cell carcinoma. Exp Ther Med. 9:961–966. 2015.PubMed/NCBI View Article : Google Scholar

28 

Feng L, Ma J, Ji H, Liu Y and Hu W: MiR-184 retarded the proliferation, invasiveness and migration of glioblastoma cells by repressing stanniocalcin-2. Pathol Oncol Res. 24:853–860. 2018.PubMed/NCBI View Article : Google Scholar

29 

Phua YW, Nguyen A, Roden DL, Elsworth B, Deng N, Nikolic I, Yang J, Mcfarland A, Russell R, Kaplan W, et al: MicroRNA profiling of the pubertal mouse mammary gland identifies miR-184 as a candidate breast tumour suppressor gene. Breast Cancer Res. 17(83)2015.PubMed/NCBI View Article : Google Scholar

30 

Chen D, Li J, Li S, Han P, Li N, Wang Y and Du S: miR-184 promotes cell proliferation in tongue squamous cell carcinoma by targeting SOX7. Oncol Lett. 16:2221–2228. 2018.PubMed/NCBI View Article : Google Scholar

31 

Schneider JA and Logan SK: Revisiting the role of Wnt/β-catenin signaling in prostate cancer. Mol Cell Endocrinol. 462:3–8. 2018.PubMed/NCBI View Article : Google Scholar

32 

Chan DW, Hui WW, Wang JJ, Yung MMH, Hui LMN, Qin Y, Liang RR, Leung THY, Xu D, Chan KKL, et al: DLX1 acts as a crucial target of FOXM1 to promote ovarian cancer aggressiveness by enhancing TGF-β/SMAD4 signaling. Oncogene. 36:1404–1416. 2017.PubMed/NCBI View Article : Google Scholar

33 

Sabóia TM, Reis MF, Martins AM, Romanos HF, Tannure PN, Granjeiro JM, Vieira AR, Antunes LS, Küchler EC and Costa MC: DLX1 and MMP3 contribute to oral clefts with and without positive family history of cancer. Arch Oral Biol. 60:223–228. 2015.PubMed/NCBI View Article : Google Scholar

34 

Leyten GH, Hessels D, Smit FP, Jannink SA, de Jong H, Melchers WJ, Cornel EB, de Reijke TM, Vergunst H, Kil P, et al: Identification of a candidate gene panel for the early diagnosis of prostate cancer. Clin Cancer Res. 21:3061–3070. 2015.PubMed/NCBI View Article : Google Scholar

35 

Rhie SK, Guo Y, Tak YG, Yao L, Shen H, Coetzee GA, Laird PW and Farnham PJ: Identification of activated enhancers and linked transcription factors in breast, prostate, and kidney tumors by tracing enhancer networks using epigenetic traits. Epigenetics Chromatin. 9(50)2016.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Tan G, Xu C, Zhong W and Wang C: miR‑184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1. Exp Ther Med 22: 1163, 2021.
APA
Tan, G., Xu, C., Zhong, W., & Wang, C. (2021). miR‑184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1. Experimental and Therapeutic Medicine, 22, 1163. https://doi.org/10.3892/etm.2021.10597
MLA
Tan, G., Xu, C., Zhong, W., Wang, C."miR‑184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1". Experimental and Therapeutic Medicine 22.4 (2021): 1163.
Chicago
Tan, G., Xu, C., Zhong, W., Wang, C."miR‑184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1163. https://doi.org/10.3892/etm.2021.10597
Copy and paste a formatted citation
x
Spandidos Publications style
Tan G, Xu C, Zhong W and Wang C: miR‑184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1. Exp Ther Med 22: 1163, 2021.
APA
Tan, G., Xu, C., Zhong, W., & Wang, C. (2021). miR‑184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1. Experimental and Therapeutic Medicine, 22, 1163. https://doi.org/10.3892/etm.2021.10597
MLA
Tan, G., Xu, C., Zhong, W., Wang, C."miR‑184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1". Experimental and Therapeutic Medicine 22.4 (2021): 1163.
Chicago
Tan, G., Xu, C., Zhong, W., Wang, C."miR‑184 delays cell proliferation, migration and invasion in prostate cancer by directly suppressing DLX1". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1163. https://doi.org/10.3892/etm.2021.10597
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team