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In vitro assessment of the cytotoxicity and anti‑inflammatory properties of a novel dietary supplement

  • Authors:
    • Ionela D. Popescu
    • Elena Codrici
    • Simona Mihai
    • Cristina-Mihaela Luntraru
    • Mihaela Neagu
    • Cristiana Tanase
  • View Affiliations / Copyright

    Affiliations: Biochemistry‑Proteomics Department, ‘Victor Babes’ National Institute of Pathology, 050096 Bucharest, Romania, Department of Research and Development Patents, SC Hofigal Export Import SA, 042124 Bucharest, Romania
    Copyright: © Popescu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1170
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    Published online on: August 12, 2021
       https://doi.org/10.3892/etm.2021.10604
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Abstract

Studies on the immunopharmacological activities of various plant species have provided evidence for the high therapeutic potential of different extracts. These represent a promising alternative to reduce the inflammatory processes and, thus, diseases related to inflammation. Numerous scientific studies strongly suggest that diet plays an essential role in inflammation, and that certain dietary factors can act as preventive or treatment methods to lower inflammation. In the present study, a novel lingonberry‑based dietary supplement was investigated for the ability to suppress the inflammatory response in activated monocytes/macrophages. Based on cell viability/proliferation and cytotoxicity tests, concentrations between 40 and 130 µg/ml of the extracts showed a high viability/proliferation effect and no cytotoxic activity in monocyte/macrophage cells. To further investigate the anti‑inflammatory potential of our novel lingonberry‑based dietary supplement, we studied the effect of the extract on the inflammatory response in lipopolysaccharide (LPS)‑stimulated macrophages. We found that the extract exhibited a strong anti‑inflammatory potential by inhibiting the expression of major inflammatory cytokines [interleukin (IL)‑6, IL‑8 and tumor necrosis factor (TNF)α] in activated monocyte/macrophage cells. The expression of IL‑6 and IL‑8 was subsequently validated by enzyme‑linked immunosorbent assay (ELISA). In conclusion, we demonstrated that our product exhibits no cytotoxicity and suppresses inflammation, and thus can be considered a natural important tool for inflammation control.
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Copy and paste a formatted citation
Spandidos Publications style
Popescu ID, Codrici E, Mihai S, Luntraru C, Neagu M and Tanase C: <em>In vitro</em> assessment of the cytotoxicity and anti‑inflammatory properties of a novel dietary supplement. Exp Ther Med 22: 1170, 2021.
APA
Popescu, I.D., Codrici, E., Mihai, S., Luntraru, C., Neagu, M., & Tanase, C. (2021). <em>In vitro</em> assessment of the cytotoxicity and anti‑inflammatory properties of a novel dietary supplement. Experimental and Therapeutic Medicine, 22, 1170. https://doi.org/10.3892/etm.2021.10604
MLA
Popescu, I. D., Codrici, E., Mihai, S., Luntraru, C., Neagu, M., Tanase, C."<em>In vitro</em> assessment of the cytotoxicity and anti‑inflammatory properties of a novel dietary supplement". Experimental and Therapeutic Medicine 22.4 (2021): 1170.
Chicago
Popescu, I. D., Codrici, E., Mihai, S., Luntraru, C., Neagu, M., Tanase, C."<em>In vitro</em> assessment of the cytotoxicity and anti‑inflammatory properties of a novel dietary supplement". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1170. https://doi.org/10.3892/etm.2021.10604
Copy and paste a formatted citation
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Spandidos Publications style
Popescu ID, Codrici E, Mihai S, Luntraru C, Neagu M and Tanase C: <em>In vitro</em> assessment of the cytotoxicity and anti‑inflammatory properties of a novel dietary supplement. Exp Ther Med 22: 1170, 2021.
APA
Popescu, I.D., Codrici, E., Mihai, S., Luntraru, C., Neagu, M., & Tanase, C. (2021). <em>In vitro</em> assessment of the cytotoxicity and anti‑inflammatory properties of a novel dietary supplement. Experimental and Therapeutic Medicine, 22, 1170. https://doi.org/10.3892/etm.2021.10604
MLA
Popescu, I. D., Codrici, E., Mihai, S., Luntraru, C., Neagu, M., Tanase, C."<em>In vitro</em> assessment of the cytotoxicity and anti‑inflammatory properties of a novel dietary supplement". Experimental and Therapeutic Medicine 22.4 (2021): 1170.
Chicago
Popescu, I. D., Codrici, E., Mihai, S., Luntraru, C., Neagu, M., Tanase, C."<em>In vitro</em> assessment of the cytotoxicity and anti‑inflammatory properties of a novel dietary supplement". Experimental and Therapeutic Medicine 22, no. 4 (2021): 1170. https://doi.org/10.3892/etm.2021.10604
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