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HBxAg promotes HBV replication and EGFR activation in human placental trophoblasts

  • Authors:
    • Yayun Lin
    • Yan Liu
    • Dongping Xu
    • Fanfan Guo
    • Wentao Zhang
    • Yidan Zhang
    • Guiqin Bai
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Institute of Infectious Diseases, 5th Medical Center of Chinese PLA General Hospital, Beijing 100141, P.R. China, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1211
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    Published online on: August 24, 2021
       https://doi.org/10.3892/etm.2021.10645
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Abstract

Hepatitis B virus (HBV) infection is a global epidemic. The main transmission route of chronic HBV infection is from mother to child, yet the mechanisms underlying HBV intrauterine infection remain unclear. In the present study, the effect and the mechanism underlying hepatitis B virus X antigen (HBxAg) on HBV replication and EGFR activation in trophoblasts was investigated. Serum samples from pregnant women with HBV infection were used to infect trophoblasts and HBxAg expression was detected using ELISA. HBV plasmids carrying either full length hepatitis B virus X (HBx) or HBx with a deletion mutation (ΔHBx) were transfected into trophoblasts and expression levels of HBV DNA, hepatitis B e‑antigen and pregenomic (pg)RNA, and structural maintenance of chromosomes (Smc) 5/6 were assessed. The association between HBx and EGFR promoters was characterized using a luciferase reporter assay and EGFR/PI3K/phosphorylated (p)‑AKT expression and apoptosis rate were also monitored. The results of the present study indicated that HBxAg expression increased with the increasing titre of HBV DNA (P<0.05). Compared with the wild‑type group, the amount of HBV DNA in the supernatant and cells was significantly reduced (P<0.05) in the ΔHBx group and the intracellular HBeAg and pgRNA levels were also significantly decreased (P<0.05). In addition, Smc5/6 expression was also significantly decreased (P<0.05) when the intracellular HBx protein was expressed compared with mock‑transfected cells. Co‑transfection of HBx and EGFR promoter plasmids in JEG‑3 and HTR‑8 cells significantly elevated EGFR promoter driven luciferase expression relative to the control group (P<0.01). In EGFR overexpressing cells, the expression of PI3K/p‑AKT was significantly increased, whereas the apoptosis rate was significantly decreased (P<0.05). These results were reversed in the EGFR‑knockdown group. In conclusion, the present study demonstrated that HBx promotes HBV replication in trophoblasts via downregulation of Smc5/6, activates the EGFR promoter and inhibits trophoblast apoptosis via the PI3K/p‑AKT downstream signalling pathway, thereby increasing the risk of HBV intrauterine infection.
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1 

Dandri M: Epigenetic modulation in chronic hepatitis B virus infection. Semin Immunopathol. 42:173–185. 2020.PubMed/NCBI View Article : Google Scholar

2 

Liu CJ and Kao JH: NOhep: Toward Global Control of Hepatitis B virus infection-an introduction. J Infect Dis. 216 (Suppl 8)(S749)2017.PubMed/NCBI View Article : Google Scholar

3 

Maini MK and Bertoletti A: HBV in 2016: Global and immunotherapeutic insights into hepatitis B. Nat Rev Gastroenterol Hepatol. 14:71–72. 2017.PubMed/NCBI View Article : Google Scholar

4 

Wu Q, Xu C, Li J, Li L, Yan G, Yue L, Zeng Y, Huang H, Deng G and Wang Y: Evolution and mutations of hepatitis B virus quasispecies in genotype B and C during vertical transmission. J Med Virol. 88:1018–1026. 2016.PubMed/NCBI View Article : Google Scholar

5 

Eke AC, Eleje GU, Eke UA, Xia Y and Liu J: Hepatitis B immunoglobulin during pregnancy for prevention of mother-to-child transmission of hepatitis B virus. The Cochrane Database Syst Rev. 2(CD008545)2017.PubMed/NCBI View Article : Google Scholar

6 

Li Y, Shen C, Yang L, Yang Y, Wang M, Li S, Chen F, Yang M, Peng L, Ma J, et al: Intra-host diversity of hepatitis B virus during mother-to-child transmission: The X gene may play a key role in virus survival in children after transmission. Arch Virol. 165:1279–1288. 2020.PubMed/NCBI View Article : Google Scholar

7 

Gong J and Liu X: Effect of HBIG combined with hepatitis B vaccine on blocking HBV transmission between mother and infant and its effect on immune cells. Exp Ther Med. 15:919–923. 2018.PubMed/NCBI View Article : Google Scholar

8 

Su WJ, Chen HL and Chang MH: Breakthrough hepatitis B Virus (HBV) infection from mother-to-infant transmission is the key problem hindering HBV eradication. J Infect Dis. 208:1036–1037. 2013.PubMed/NCBI View Article : Google Scholar

9 

Liu J, Feng Y, Wang J, Li X, Lei C, Jin D, Feng W, Yang Y, He Y, Li Y, et al: An ‘immune barrier’ is formed in the placenta by hepatitis B immunoglobulin to protect the fetus from hepatitis B virus infection from the mother. Hum Vaccin Immunother. 11:2068–2076. 2015.PubMed/NCBI View Article : Google Scholar

10 

Wang DD, Yi LZ, Wu LN, Yang ZQ, Hao HY, Shi XH, Wang B, Feng SY, Feng YL and Wang SP: Relationship between maternal PBMC HBV cccDNA and HBV serological markers and its effect on HBV intrauterine transmission. Biomed Environ Sci. 32:315–323. 2019.PubMed/NCBI View Article : Google Scholar

11 

Bai GQ, Li SH, Yue YF and Shi L: The study on role of peripheral blood mononuclear cell in HBV intrauterine infection. Arch Gynecol Obstet. 283:317–321. 2011.PubMed/NCBI View Article : Google Scholar

12 

Ke C, Xiao X, Gan L and Zhou Y: Preliminary study of hepatitis B virus replication in primary placental trophoblastic cells. Prog Obstet Gynecol. 24:1–5. 2015.

13 

Chen Y, Wang L, Xu Y, Liu X, Li S, Qian Q, Hu B, Zhou A, Chen T and Zhao Y: Role of maternal viremia and placental infection in hepatitis B virus intrauterine transmission. Microbes Infect. 15:409–415. 2013.PubMed/NCBI View Article : Google Scholar

14 

Zhao Y, Di F and Zhong Y: Progress in research on mechanism of HBV intrauterine transmission. Chin J Clin (Electronic Edition). 9:1433–1436. 2015.

15 

Zhang SL, Yue YF, Bai GQ, Shi L and Jiang H: Mechanism of intrauterine infection of hepatitis B virus. World J Gastroenterol. 10:437–438. 2004.PubMed/NCBI View Article : Google Scholar

16 

Zuo Y, Li D, Xu D, Chen C and Wang X: Meta-analysis of intrauterine infection rate of HBV in different periods of pregnancy. J Fourth Military Med Univ. 9:853–855. 2002.(In Chinese).

17 

Feitelson MA, Bonamassa B and Arzumanyan A: The roles of hepatitis B virus-encoded X protein in virus replication and the pathogenesis of chronic liver disease. Expert Opin Ther Targets. 18:293–306. 2014.PubMed/NCBI View Article : Google Scholar

18 

Lee HR, Cho YY, Lee GY, You DG, Yoo YD and Kim YJ: A direct role for hepatitis B virus X protein in inducing mitochondrial membrane permeabilization. J Viral Hepat. 25:412–420. 2018.PubMed/NCBI View Article : Google Scholar

19 

Zoulim F, Saputelli J and Seeger C: Woodchuck hepatitis virus X protein is required for viral infection in vivo. J Virol. 68:2026–2030. 1994.PubMed/NCBI View Article : Google Scholar

20 

Lucifora J, Arzberger S, Durantel D, Belloni L, Strubin M, Levrero M, Zoulim F, Hantz O and Protzer U: Hepatitis B virus X protein is essential to initiate and maintain virus replication after infection. J Hepatol. 55:996–1003. 2011.PubMed/NCBI View Article : Google Scholar

21 

Decorsiere A, Mueller H, van Breugel PC, Abdul F, Gerossier L, Beran RK, Livingston CM, Niu C, Fletcher SP, Hantz O and Strubin M: Hepatitis B virus X protein identifies the Smc5/6 complex as a host restriction factor. Nature. 531:386–389. 2016.PubMed/NCBI View Article : Google Scholar

22 

Mitra B and Guo H: Hepatitis B virus X protein crosses out Smc5/6 complex to maintain covalently closed circular DNA transcription. Hepatology. 64:2246–2249. 2016.PubMed/NCBI View Article : Google Scholar

23 

Murphy CM, Xu Y, Li F, Nio K, Reszka-Blanco N, Li X, Wu Y, Yu Y, Xiong Y and Su L: Hepatitis B virus X protein promotes degradation of SMC5/6 to enhance HBV replication. Cell Rep. 16:2846–2854. 2016.PubMed/NCBI View Article : Google Scholar

24 

Bai G, Wang Y, Zhang L, Tang Y and Fu F: The study on the role of hepatitis B virus X protein and apoptosis in HBV intrauterine infection. Arch Gynecol Obstet. 285:943–949. 2012.PubMed/NCBI View Article : Google Scholar

25 

Bai G, Fu F, Tang Y and Wang Y: Effect of hepatitis B virus infection on apoptosis of a human choriocarcinoma cell line in vitro. J Obstet Gynaecol Res. 39:1200–1211. 2013.PubMed/NCBI View Article : Google Scholar

26 

Hung CM, Huang WC, Pan HL, Chien PH, Lin CW, Chen LC, Chien YF, Lin CC, Leow KH, Chen WS, et al: Hepatitis B Virus X upregulates HuR protein level to stabilize HER2 expression in hepatocellular carcinoma cells. Biomed Res Int. 2014(827415)2014.PubMed/NCBI View Article : Google Scholar

27 

Chen JY, Chen YJ, Yen CJ, Chen WS and Huang WC: HBx sensitizes hepatocellular carcinoma cells to lapatinib by up-regulating ErbB3. Oncotarget. 7:473–489. 2016.PubMed/NCBI View Article : Google Scholar

28 

Graham CH, Hawley TS, Hawley RG, MacDougall JR, Kerbel RS, Khoo N and Lala PK: Establishment and characterization of first trimester human trophoblast cells with extended lifespan. Exp Cell Res. 206:204–211. 1993.PubMed/NCBI View Article : Google Scholar

29 

Abou-Kheir W, Barrak J, Hadadeh O and Daoud G: HTR-8/SVneo cell line contains a mixed population of cells. Placenta. 50:1–7. 2017.PubMed/NCBI View Article : Google Scholar

30 

Wang J, Shen T, Huang X, Kumar GR, Chen X, Zeng Z, Zhang R, Chen R, Li T, Zhang T, et al: Serum hepatitis B virus RNA is encapsidated pregenome RNA that may be associated with persistence of viral infection and rebound. J Hepatol. 65:700–710. 2016.PubMed/NCBI View Article : Google Scholar

31 

Huang H, Wang J, Li W, Chen R, Chen X, Zhang F, Xu D and Lu F: Serum HBV DNA plus RNA shows superiority in reflecting the activity of intrahepatic cccDNA in treatment-naïve HBV-infected individuals. J Clin Virol. 99-100:71–78. 2018.PubMed/NCBI View Article : Google Scholar

32 

Ma L, Alla NR, Li X, Mynbaev OA and Shi Z: Mother-to-child transmission of HBV: Review of current clinical management and prevention strategies. Rev Med Virol. 24:396–406. 2014.PubMed/NCBI View Article : Google Scholar

33 

Mavilia MG and Wu GY: Mechanisms and prevention of vertical transmission in chronic viral hepatitis. J Clin Transl Hepatol. 5:119–129. 2017.PubMed/NCBI View Article : Google Scholar

34 

Peng S, Wan Z, Liu T, Zhu H and Du Y: Incidence and risk factors of intrauterine transmission among pregnant women with chronic hepatitis B virus infection. J Clin Gastroenterol. 53:51–57. 2019.PubMed/NCBI View Article : Google Scholar

35 

Xiao Y, Sun K, Duan Z, Liu Z, Li Y, Yan L, Song Y, Zou H, Zhuang H, Wang J and Li J: Quasispecies characteristic in ‘a’ determinant region is a potential predictor for the risk of immunoprophylaxis failure of mother-to-child-transmission of sub-genotype C2 hepatitis B virus: A prospective nested case-control study. Gut. 69:933–941. 2020.PubMed/NCBI View Article : Google Scholar

36 

Shih YF and Liu CJ: Mother-to-infant transmission of hepatitis B virus: Challenges and perspectives. Hepatol Int. 11:481–484. 2017.PubMed/NCBI View Article : Google Scholar

37 

Allweiss L and Dandri M: The role of cccDNA in HBV maintenance. Viruses. 9(156)2017.PubMed/NCBI View Article : Google Scholar

38 

Giersch K, Allweiss L, Volz T, Dandri M and Lutgehetmann M: Serum HBV pgRNA as a clinical marker for cccDNA activity. J Hepatol. 66:460–462. 2017.PubMed/NCBI View Article : Google Scholar

39 

Abdul F, Filleton F, Gerossier L, Paturel A, Hall J, Strubin M and Etienne L: Smc5/6 antagonism by HBx is an evolutionarily conserved function of Hepatitis B virus infection in mammals. J Virol. 92:e00769–18. 2018.PubMed/NCBI View Article : Google Scholar

40 

Liang TJ: Virology: The X-Files of hepatitis B. Nature. 531:313–314. 2016.PubMed/NCBI View Article : Google Scholar

41 

Jeppsson K, Carlborg KK, Nakato R, Berta DG, Lilienthal I, Kanno T, Lindqvist A, Brink MC, Dantuma NP, Katou Y, et al: The chromosomal association of the Smc5/6 complex depends on cohesion and predicts the level of sister chromatid entanglement. PLoS Genet. 10(e1004680)2014.PubMed/NCBI View Article : Google Scholar

42 

Jeppsson K, Kanno T, Shirahige K and Sjogren C: The maintenance of chromosome structure: Positioning and functioning of SMC complexes. Nature reviews. Molecular cell biology. 15:601–614. 2014.PubMed/NCBI View Article : Google Scholar

43 

Van Breugel PC, Robert EI, Mueller H, Decorsière A, Zoulim F, Hantz O and Strubin M: Hepatitis B virus X protein stimulates gene expression selectively from extrachromosomal DNA templates. Hepatology. 56:2116–2124. 2012.PubMed/NCBI View Article : Google Scholar

44 

Wang W, Shi Y, Bai G, Tang Y, Yuan Y, Zhang T and Li C: HBxAg suppresses apoptosis of human placental trophoblastic cell lines via activation of the PI3K/Akt pathway. Cell Biol Int. 40:708–715. 2016.PubMed/NCBI View Article : Google Scholar

45 

Chen YJ, Chien PH, Chen WS, Chien YF, Hsu YY, Wang LY, Chen JY, Lin CW, Huang TC, Yu YL and Huang WC: Hepatitis B virus-encoded X protein downregulates EGFR expression via inducing MicroRNA-7 in hepatocellular carcinoma cells. Evid Based Complement Alternat Med. 2013(682380)2013.PubMed/NCBI View Article : Google Scholar

46 

Wee P and Wang Z: Epidermal growth factor receptor cell proliferation signaling pathways. Cancers (Basel). 9(52)2017.PubMed/NCBI View Article : Google Scholar

47 

Wang W, Bai G, Zhang Y, Zhang T, Li C, Yuan Y, Liu S and Wang C: HBxAg suppresses cell apoptosis and promotes the secretion of placental hormones in human placental trophoblasts via activation of the EGFR/Akt pathway. Cell Biol Int. 42:237–247. 2018.PubMed/NCBI View Article : Google Scholar

48 

Rawat S and Bouchard MJ: The hepatitis B virus (HBV) HBx protein activates AKT to simultaneously regulate HBV replication and hepatocyte survival. J Virol. 89:999–1012. 2015.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Lin Y, Liu Y, Xu D, Guo F, Zhang W, Zhang Y and Bai G: HBxAg promotes HBV replication and EGFR activation in human placental trophoblasts. Exp Ther Med 22: 1211, 2021.
APA
Lin, Y., Liu, Y., Xu, D., Guo, F., Zhang, W., Zhang, Y., & Bai, G. (2021). HBxAg promotes HBV replication and EGFR activation in human placental trophoblasts. Experimental and Therapeutic Medicine, 22, 1211. https://doi.org/10.3892/etm.2021.10645
MLA
Lin, Y., Liu, Y., Xu, D., Guo, F., Zhang, W., Zhang, Y., Bai, G."HBxAg promotes HBV replication and EGFR activation in human placental trophoblasts". Experimental and Therapeutic Medicine 22.5 (2021): 1211.
Chicago
Lin, Y., Liu, Y., Xu, D., Guo, F., Zhang, W., Zhang, Y., Bai, G."HBxAg promotes HBV replication and EGFR activation in human placental trophoblasts". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1211. https://doi.org/10.3892/etm.2021.10645
Copy and paste a formatted citation
x
Spandidos Publications style
Lin Y, Liu Y, Xu D, Guo F, Zhang W, Zhang Y and Bai G: HBxAg promotes HBV replication and EGFR activation in human placental trophoblasts. Exp Ther Med 22: 1211, 2021.
APA
Lin, Y., Liu, Y., Xu, D., Guo, F., Zhang, W., Zhang, Y., & Bai, G. (2021). HBxAg promotes HBV replication and EGFR activation in human placental trophoblasts. Experimental and Therapeutic Medicine, 22, 1211. https://doi.org/10.3892/etm.2021.10645
MLA
Lin, Y., Liu, Y., Xu, D., Guo, F., Zhang, W., Zhang, Y., Bai, G."HBxAg promotes HBV replication and EGFR activation in human placental trophoblasts". Experimental and Therapeutic Medicine 22.5 (2021): 1211.
Chicago
Lin, Y., Liu, Y., Xu, D., Guo, F., Zhang, W., Zhang, Y., Bai, G."HBxAg promotes HBV replication and EGFR activation in human placental trophoblasts". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1211. https://doi.org/10.3892/etm.2021.10645
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