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Resolvin D2 suppresses NLRP3 inflammasome by promoting autophagy in macrophages

  • Authors:
    • Lijun Cao
    • Yiya Wang
    • Yina Wang
    • Feijuan Lv
    • Lixiu Liu
    • Zhen Li
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, No. 906 Hospital of the Chinese People's Liberation Army Joint Logistic Support Force, Ningbo, Zhejiang 315040, P.R. China, Department of Anesthesiology, No. 906 Hospital of the Chinese People's Liberation Army Joint Logistic Support Force, Ningbo, Zhejiang 315040, P.R. China, Department of Emergency, No. 906 Hospital of the Chinese People's Liberation Army Joint Logistic Support Force, Ningbo, Zhejiang 315040, P.R. China, Department of Endocrinology, No. 906 Hospital of the Chinese People's Liberation Army Joint Logistic Support Force, Ningbo, Zhejiang 315040, P.R. China, Department of Emergency, No. 906 Hospital of the Chinese People's Liberation Army Joint Logistic Support Force, Ningbo, Zhejiang 315040, P.R. China
    Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1222
    |
    Published online on: August 26, 2021
       https://doi.org/10.3892/etm.2021.10656
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Abstract

Inflammasome, a multiprotein complex that regulates interleukin (IL)‑1β secretion and pyroptosis, participates in numerous inflammatory diseases, including sepsis, atherosclerosis and type‑2 diabetes. Investigating the inflammasome regulation is therefore crucial to understand the inflammasome activation and develop treatment for the related diseases. In addition, it remains unknown how the inflammasome is naturally suppressed during the inflammatory process. The present study aimed to investigate the role of resolvin D2 (RvD2), an innate suppressor of inflammation produced from essential ω3‑polyunsaturated fatty acids, in the activation of the inflammasome via in vitro and in vivo experiments. The effects of RvD2 on the cytokine production of inflammasome‑related peritonitis were determined, and the NLRP3 inflammasome activation was investigated in the presence of RvD2. Moreover, the potential mechanisms underlying RvD2 in NLRP3 inflammasome regulation through autophagy and proteasome were investigated. The results of the present study demonstrated that RvD2 suppressed inflammasome‑mediated peritonitis in vivo and regulated the NLR family pyrin domain containing 3 (NLRP3) inflammasome, but not in absent in melanoma 2 (AIM2), NLR family CARD domain containing 4 (NLRC4) inflammasomes. Mechanistically, RvD2 was found to promote the degradation of NLRP3 through autophagy, and the inhibition of autophagy could reverse the RvD2‑mediated suppression of NLRP3 inflammasome in vitro and partially reverse the inflammasome‑mediated peritonitis in vivo. In summary, the present study reported the negative regulation of NLRP3 inflammasome activation by RvD2. The findings from this study may extend the knowledge of the innate regulation of inflammasome and highlight a possible target for inflammasome‑related diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Cao L, Wang Y, Wang Y, Lv F, Liu L and Li Z: Resolvin D2 suppresses NLRP3 inflammasome by promoting autophagy in macrophages. Exp Ther Med 22: 1222, 2021.
APA
Cao, L., Wang, Y., Wang, Y., Lv, F., Liu, L., & Li, Z. (2021). Resolvin D2 suppresses NLRP3 inflammasome by promoting autophagy in macrophages. Experimental and Therapeutic Medicine, 22, 1222. https://doi.org/10.3892/etm.2021.10656
MLA
Cao, L., Wang, Y., Wang, Y., Lv, F., Liu, L., Li, Z."Resolvin D2 suppresses NLRP3 inflammasome by promoting autophagy in macrophages". Experimental and Therapeutic Medicine 22.5 (2021): 1222.
Chicago
Cao, L., Wang, Y., Wang, Y., Lv, F., Liu, L., Li, Z."Resolvin D2 suppresses NLRP3 inflammasome by promoting autophagy in macrophages". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1222. https://doi.org/10.3892/etm.2021.10656
Copy and paste a formatted citation
x
Spandidos Publications style
Cao L, Wang Y, Wang Y, Lv F, Liu L and Li Z: Resolvin D2 suppresses NLRP3 inflammasome by promoting autophagy in macrophages. Exp Ther Med 22: 1222, 2021.
APA
Cao, L., Wang, Y., Wang, Y., Lv, F., Liu, L., & Li, Z. (2021). Resolvin D2 suppresses NLRP3 inflammasome by promoting autophagy in macrophages. Experimental and Therapeutic Medicine, 22, 1222. https://doi.org/10.3892/etm.2021.10656
MLA
Cao, L., Wang, Y., Wang, Y., Lv, F., Liu, L., Li, Z."Resolvin D2 suppresses NLRP3 inflammasome by promoting autophagy in macrophages". Experimental and Therapeutic Medicine 22.5 (2021): 1222.
Chicago
Cao, L., Wang, Y., Wang, Y., Lv, F., Liu, L., Li, Z."Resolvin D2 suppresses NLRP3 inflammasome by promoting autophagy in macrophages". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1222. https://doi.org/10.3892/etm.2021.10656
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