Anti‑inflammatory effects and enhancing immune response of freshwater hybrid catfish oil in RAW264.7 cells
- Bussarin Tongmee
- Atcharaporn Ontawong
- Narissara Lailerd
- Kriangsak Mengamphan
- Doungporn Amornlerdpisan
Affiliations: Agricultural Interdisciplinary Program, Faculty of Engineer and Agro‑Industry, Maejo University, Chiang Mai 50290, Thailand, Division of Physiology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand, Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand, Center of Excellence in Agricultural Innovation for Graduate Entrepreneur, Maejo University, Chiang Mai 50290, Thailand
- Published online on: August 26, 2021 https://doi.org/10.3892/etm.2021.10657
Copyright: © Tongmee
et al. This is an open access article distributed under the
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The present study assessed the effect of freshwater hybrid catfish oil (FFO) on the inflammatory status of lipopolysaccharide (LPS)‑stimulated RAW264.7 cells and investigated the underlying mechanisms. RAW264.7 cells were supplemented with various concentrations [0.125‑2% in 0.5% propylene glycol (v/v)] of FFO with or without LPS (1 µg/ml) for 24 h. Inflammatory cytokines and mediators were quantified using ELISA and reverse transcription‑quantitative PCR. The results revealed that FFO treatment inhibited the secretion and mRNA expression of the pro‑inflammatory cytokines IL‑6, IL‑1β, TNF‑α. In line with this, FFO suppressed the expression and secretion of the inflammatory mediators cyclooxygenase‑2 and prostaglandin E2. FFO also reduced apoptotic body formation and DNA damage. Correspondingly, FFO enhanced the immune response by modulating the cell cycle regulators p53, cyclin D2 and cyclin E2. Accordingly, FFO may be developed as a nutraceutical product to prevent inflammation.