Open Access

Anti‑inflammatory effects and enhancing immune response of freshwater hybrid catfish oil in RAW264.7 cells

  • Authors:
    • Bussarin Tongmee
    • Atcharaporn Ontawong
    • Narissara Lailerd
    • Kriangsak Mengamphan
    • Doungporn Amornlerdpisan
  • View Affiliations

  • Published online on: August 26, 2021     https://doi.org/10.3892/etm.2021.10657
  • Article Number: 1223
  • Copyright: © Tongmee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study assessed the effect of freshwater hybrid catfish oil (FFO) on the inflammatory status of lipopolysaccharide (LPS)‑stimulated RAW264.7 cells and investigated the underlying mechanisms. RAW264.7 cells were supplemented with various concentrations [0.125‑2% in 0.5% propylene glycol (v/v)] of FFO with or without LPS (1 µg/ml) for 24 h. Inflammatory cytokines and mediators were quantified using ELISA and reverse transcription‑quantitative PCR. The results revealed that FFO treatment inhibited the secretion and mRNA expression of the pro‑inflammatory cytokines IL‑6, IL‑1β, TNF‑α. In line with this, FFO suppressed the expression and secretion of the inflammatory mediators cyclooxygenase‑2 and prostaglandin E2. FFO also reduced apoptotic body formation and DNA damage. Correspondingly, FFO enhanced the immune response by modulating the cell cycle regulators p53, cyclin D2 and cyclin E2. Accordingly, FFO may be developed as a nutraceutical product to prevent inflammation.
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November-2021
Volume 22 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Tongmee B, Ontawong A, Lailerd N, Mengamphan K and Amornlerdpisan D: Anti‑inflammatory effects and enhancing immune response of freshwater hybrid catfish oil in RAW264.7 cells. Exp Ther Med 22: 1223, 2021
APA
Tongmee, B., Ontawong, A., Lailerd, N., Mengamphan, K., & Amornlerdpisan, D. (2021). Anti‑inflammatory effects and enhancing immune response of freshwater hybrid catfish oil in RAW264.7 cells. Experimental and Therapeutic Medicine, 22, 1223. https://doi.org/10.3892/etm.2021.10657
MLA
Tongmee, B., Ontawong, A., Lailerd, N., Mengamphan, K., Amornlerdpisan, D."Anti‑inflammatory effects and enhancing immune response of freshwater hybrid catfish oil in RAW264.7 cells". Experimental and Therapeutic Medicine 22.5 (2021): 1223.
Chicago
Tongmee, B., Ontawong, A., Lailerd, N., Mengamphan, K., Amornlerdpisan, D."Anti‑inflammatory effects and enhancing immune response of freshwater hybrid catfish oil in RAW264.7 cells". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1223. https://doi.org/10.3892/etm.2021.10657