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Butorphanol alleviates lipopolysaccharide‑induced inflammation and apoptosis of cardiomyocytes via activation of the κ‑opioid receptor

  • Authors:
    • Weiqing Tang
    • Liu Luo
    • Baoji Hu
    • Mingzhi Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 201399, P.R. China, Department of Anesthesiology, The Affiliated Zhuzhou Hospital of Xiangya School of Medicine, Central South University, Zhuzhou Central Hospital, Zhuzhou, Hunan 412000, P.R. China
    Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1248
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    Published online on: September 2, 2021
       https://doi.org/10.3892/etm.2021.10683
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Abstract

Sepsis‑induced myocardial dysfunction is a leading cause of the high mortality rates associated with sepsis. The aim of the present study was to investigate the effect of butorphanol on sepsis‑induced cardiomyocyte dysfunction. Lipopolysaccharide (LPS) was used to induce H9C2 cardiomyocytes to establish an in vitro sepsis model. The effect of butorphanol on the viability of LPS‑induced H9C2 cells was analyzed using a Cell Counting Kit‑8 assay. The levels of tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6 were detected using ELISA. Western blotting was used to analyze the expression levels of inflammation‑and apoptosis‑related proteins. Cell apoptosis was measured using a TUNEL assay. The expression levels of κ‑opioid receptor (KOR) were analyzed using reverse transcription‑quantitative PCR analysis and western blotting. Following LPS induction, the levels of inflammatory cytokines and proapoptotic proteins were found to be upregulated in H9C2 cells, while butorphanol treatment downregulated these levels. The expression levels of KOR were also upregulated following butorphanol treatment in LPS‑induced H9C2 cells. Addition of the KOR inhibitor, nor‑binaltorphimine, alleviated the inhibitory effects of butorphanol on inflammation and apoptosis in LPS‑induced H9C2 cells. In conclusion, the findings of the present study provided evidence indicating that butorphanol may alleviate LPS‑induced inflammation and apoptosis in cardiomyocytes by upregulating KOR expression, which may provide a novel insight into the potential therapeutic effects of butorphanol and its underlying mechanism of action.
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Copy and paste a formatted citation
Spandidos Publications style
Tang W, Luo L, Hu B and Zheng M: Butorphanol alleviates lipopolysaccharide‑induced inflammation and apoptosis of cardiomyocytes via activation of the κ‑opioid receptor. Exp Ther Med 22: 1248, 2021.
APA
Tang, W., Luo, L., Hu, B., & Zheng, M. (2021). Butorphanol alleviates lipopolysaccharide‑induced inflammation and apoptosis of cardiomyocytes via activation of the κ‑opioid receptor. Experimental and Therapeutic Medicine, 22, 1248. https://doi.org/10.3892/etm.2021.10683
MLA
Tang, W., Luo, L., Hu, B., Zheng, M."Butorphanol alleviates lipopolysaccharide‑induced inflammation and apoptosis of cardiomyocytes via activation of the κ‑opioid receptor". Experimental and Therapeutic Medicine 22.5 (2021): 1248.
Chicago
Tang, W., Luo, L., Hu, B., Zheng, M."Butorphanol alleviates lipopolysaccharide‑induced inflammation and apoptosis of cardiomyocytes via activation of the κ‑opioid receptor". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1248. https://doi.org/10.3892/etm.2021.10683
Copy and paste a formatted citation
x
Spandidos Publications style
Tang W, Luo L, Hu B and Zheng M: Butorphanol alleviates lipopolysaccharide‑induced inflammation and apoptosis of cardiomyocytes via activation of the κ‑opioid receptor. Exp Ther Med 22: 1248, 2021.
APA
Tang, W., Luo, L., Hu, B., & Zheng, M. (2021). Butorphanol alleviates lipopolysaccharide‑induced inflammation and apoptosis of cardiomyocytes via activation of the κ‑opioid receptor. Experimental and Therapeutic Medicine, 22, 1248. https://doi.org/10.3892/etm.2021.10683
MLA
Tang, W., Luo, L., Hu, B., Zheng, M."Butorphanol alleviates lipopolysaccharide‑induced inflammation and apoptosis of cardiomyocytes via activation of the κ‑opioid receptor". Experimental and Therapeutic Medicine 22.5 (2021): 1248.
Chicago
Tang, W., Luo, L., Hu, B., Zheng, M."Butorphanol alleviates lipopolysaccharide‑induced inflammation and apoptosis of cardiomyocytes via activation of the κ‑opioid receptor". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1248. https://doi.org/10.3892/etm.2021.10683
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