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Knockdown of lncRNA TUG1 protects lens epithelial cells from oxidative stress‑induced injury by regulating miR‑196a‑5p expression in age‑related cataracts

  • Authors:
    • Qimin Shen
    • Tian Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, People's Hospital of Yuyao, Ningbo, Zhejiang 315400, P.R. China
    Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1286
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    Published online on: September 13, 2021
       https://doi.org/10.3892/etm.2021.10721
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Abstract

Oxidative stress plays an important role in the pathogenesis of cataracts. Under oxidative stress, apoptosis of lens epithelial cells (LECs) is activated, which may cause lens opacity and accelerate the development of cataracts. Long non‑coding RNA (lncRNA) and microRNA (miRNA/miR) are involved in cataracts. Previous studies have demonstrated that lncRNA taurine upregulated 1 (TUG1) promotes cell apoptosis induced by ultraviolet radiation by downregulating the expression of miR‑421. However, the mechanism underlying TUG1 in age‑related cataract remains to be elucidated. The present study aimed to investigate the effect of TUG1 in age‑related cataracts and to determine the related underlying molecular mechanism. In the present study, the association between TUG1 and microRNA (miR)‑196a‑5p was predicted using StarBase and verified using a dual luciferase reporter assay in 293 cells. The LEC line SRA01/04 was exposed to 200 µM hydrogen peroxide (H2O2) for 24 h to establish an in vitro oxidative stress model. The mRNA expression levels of TUG1 and miR‑196a‑5p were analyzed using reverse transcription‑quantitative PCR, whilst cell viability and apoptosis were determined using MTT and flow cytometry assays, respectively. The protein expression levels of cleaved caspase‑3 and caspase‑3 in SRA01/04 cells were determined using western blotting. The results of the present study revealed that TUG1 directly targeted miR‑196a‑5p expression. In addition, the expression levels of miR‑196a‑5p were downregulated in SRA01/04 cells following oxidative stress, whilst TUG1 expression was upregulated. Cell transfection with TUG1‑small interfering RNA (siRNA) upregulated miR‑196a‑5p expression levels in SRA01/04 cells, which was reversed following co‑transfection with the miR‑196a‑5p inhibitor. Transfection with TUG1‑siRNA also reduced the levels of H2O2‑induced oxidative damage in SRA01/04 cells, which was demonstrated by increased cell viability, reduced levels of apoptosis and downregulated cleaved caspase‑3 levels. Conversely, transfection with the miR‑196a‑5p inhibitor reversed these effects aforementioned. Overexpression of miR‑196a‑5p reduced H2O2‑induced oxidative damage in SRA01/04 cells. In conclusion, findings from the present study suggested that knocking down TUG1 expression may protect LECs from oxidative stress‑induced apoptosis by upregulating the expression of miR‑196a‑5p.
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Copy and paste a formatted citation
Spandidos Publications style
Shen Q and Zhou T: Knockdown of lncRNA TUG1 protects lens epithelial cells from oxidative stress‑induced injury by regulating miR‑196a‑5p expression in age‑related cataracts. Exp Ther Med 22: 1286, 2021.
APA
Shen, Q., & Zhou, T. (2021). Knockdown of lncRNA TUG1 protects lens epithelial cells from oxidative stress‑induced injury by regulating miR‑196a‑5p expression in age‑related cataracts. Experimental and Therapeutic Medicine, 22, 1286. https://doi.org/10.3892/etm.2021.10721
MLA
Shen, Q., Zhou, T."Knockdown of lncRNA TUG1 protects lens epithelial cells from oxidative stress‑induced injury by regulating miR‑196a‑5p expression in age‑related cataracts". Experimental and Therapeutic Medicine 22.5 (2021): 1286.
Chicago
Shen, Q., Zhou, T."Knockdown of lncRNA TUG1 protects lens epithelial cells from oxidative stress‑induced injury by regulating miR‑196a‑5p expression in age‑related cataracts". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1286. https://doi.org/10.3892/etm.2021.10721
Copy and paste a formatted citation
x
Spandidos Publications style
Shen Q and Zhou T: Knockdown of lncRNA TUG1 protects lens epithelial cells from oxidative stress‑induced injury by regulating miR‑196a‑5p expression in age‑related cataracts. Exp Ther Med 22: 1286, 2021.
APA
Shen, Q., & Zhou, T. (2021). Knockdown of lncRNA TUG1 protects lens epithelial cells from oxidative stress‑induced injury by regulating miR‑196a‑5p expression in age‑related cataracts. Experimental and Therapeutic Medicine, 22, 1286. https://doi.org/10.3892/etm.2021.10721
MLA
Shen, Q., Zhou, T."Knockdown of lncRNA TUG1 protects lens epithelial cells from oxidative stress‑induced injury by regulating miR‑196a‑5p expression in age‑related cataracts". Experimental and Therapeutic Medicine 22.5 (2021): 1286.
Chicago
Shen, Q., Zhou, T."Knockdown of lncRNA TUG1 protects lens epithelial cells from oxidative stress‑induced injury by regulating miR‑196a‑5p expression in age‑related cataracts". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1286. https://doi.org/10.3892/etm.2021.10721
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