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LIPOSA pharmacopuncture, a new herbal formula, affects localized adiposity by regulating lipid metabolism in vivo

  • Authors:
    • Haesu Lee
    • Mi Hye Kim
    • Seong Chul Jin
    • La Yoon Choi
    • Yeon Kyung Nam
    • Woong Mo Yang
  • View Affiliations / Copyright

    Affiliations: Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1290
    |
    Published online on: September 13, 2021
       https://doi.org/10.3892/etm.2021.10725
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Abstract

Localized adiposity is a serious aesthetic problem and a well‑known health risk factor. There is a growing interest in minimally invasive treatment options for excessive fat accumulation, such as pharmacopuncture. LIPOSA is a newly developed pharmacopuncture formula from three natural herbs: The tuber of Pinellia ternata (Thunb.) Breitenb., the whole plant of Taraxacum platycarpum Dahlst. and the root of Astragalus membranaceus Bunge. The present study investigated the effects of pharmacopuncture treatment with LIPOSA on localized adiposity. Male C57BL/6J mice were fed high fat diet for 8 weeks to induce obesity. Then, 100 µl LIPOSA was injected into the left‑side inguinal fat pad at various concentrations, including 13.35, 26.7 and 53.4 mg/ml. Normal saline was injected into the right‑side inguinal fat pad of each mouse as a control. The treatment was performed three times per week for 2 weeks. The weight and histological changes were analyzed in the inguinal fat pad of the obese mice. The expression levels of adipose triglyceride lipase (ATGL), hormone‑sensitive lipase (HSL), autophagy‑related gene (ATG)5, ATG7 and LC3‑II, as lipophagy‑related factors, were evaluated to confirm the lipid‑catabolic effects of LIPOSA. LIPOSA pharmacopuncture markedly decreased the weight of the fat tissue and the size of the adipocytes in the inguinal region of the mouse models of obesity in a dose‑dependent manner. The expression levels of ATGL, HSL, ATG5, ATG7 and LC3‑II were significantly increased by the LIPOSA treatments. In addition, LIPOSA pharmacopuncture was found to decrease the expression levels of ACC, PPAR‑γ and PEPCK. The results indicated that subcutaneous injection of LIPOSA can degrade local fat and induce lipophagic and lipase activation effects. In addition, lipid metabolism related to fat accumulation was regulated by the LIPOSA treatment. The present study suggests that LIPOSA pharmacopuncture can be a non‑surgical alternative in the treatment of localized adiposity.
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Copy and paste a formatted citation
Spandidos Publications style
Lee H, Kim MH, Jin SC, Choi LY, Nam YK and Yang WM: LIPOSA pharmacopuncture, a new herbal formula, affects localized adiposity by regulating lipid metabolism <em>in vivo</em>. Exp Ther Med 22: 1290, 2021.
APA
Lee, H., Kim, M.H., Jin, S.C., Choi, L.Y., Nam, Y.K., & Yang, W.M. (2021). LIPOSA pharmacopuncture, a new herbal formula, affects localized adiposity by regulating lipid metabolism <em>in vivo</em>. Experimental and Therapeutic Medicine, 22, 1290. https://doi.org/10.3892/etm.2021.10725
MLA
Lee, H., Kim, M. H., Jin, S. C., Choi, L. Y., Nam, Y. K., Yang, W. M."LIPOSA pharmacopuncture, a new herbal formula, affects localized adiposity by regulating lipid metabolism <em>in vivo</em>". Experimental and Therapeutic Medicine 22.5 (2021): 1290.
Chicago
Lee, H., Kim, M. H., Jin, S. C., Choi, L. Y., Nam, Y. K., Yang, W. M."LIPOSA pharmacopuncture, a new herbal formula, affects localized adiposity by regulating lipid metabolism <em>in vivo</em>". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1290. https://doi.org/10.3892/etm.2021.10725
Copy and paste a formatted citation
x
Spandidos Publications style
Lee H, Kim MH, Jin SC, Choi LY, Nam YK and Yang WM: LIPOSA pharmacopuncture, a new herbal formula, affects localized adiposity by regulating lipid metabolism <em>in vivo</em>. Exp Ther Med 22: 1290, 2021.
APA
Lee, H., Kim, M.H., Jin, S.C., Choi, L.Y., Nam, Y.K., & Yang, W.M. (2021). LIPOSA pharmacopuncture, a new herbal formula, affects localized adiposity by regulating lipid metabolism <em>in vivo</em>. Experimental and Therapeutic Medicine, 22, 1290. https://doi.org/10.3892/etm.2021.10725
MLA
Lee, H., Kim, M. H., Jin, S. C., Choi, L. Y., Nam, Y. K., Yang, W. M."LIPOSA pharmacopuncture, a new herbal formula, affects localized adiposity by regulating lipid metabolism <em>in vivo</em>". Experimental and Therapeutic Medicine 22.5 (2021): 1290.
Chicago
Lee, H., Kim, M. H., Jin, S. C., Choi, L. Y., Nam, Y. K., Yang, W. M."LIPOSA pharmacopuncture, a new herbal formula, affects localized adiposity by regulating lipid metabolism <em>in vivo</em>". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1290. https://doi.org/10.3892/etm.2021.10725
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