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Exosomes derived from PC‑3 cells suppress osteoclast differentiation by downregulating miR‑148a and blocking the PI3K/AKT/mTOR pathway

  • Authors:
    • Gaoqiang Tian
    • Konghe Hu
    • Sujun Qiu
    • Yingming Xie
    • Yanlin Cao
    • Songjia Ni
    • Lifang Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Spine Surgery, The Affiliated Yuebei People's Hospital of Shantou University Medical College, Shaoguan, Guangdong 512025, P.R. China, Department of Orthopedics, Zhujiang Hospital, The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Southern Medical University, Guangzhou, Guangdong 510250, P.R. China
    Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1304
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    Published online on: September 16, 2021
       https://doi.org/10.3892/etm.2021.10739
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Abstract

Prostate cancer is a leading malignancy in men that can also disrupt the bone tissue balance. Among all urological cancers, prostate cancer is associated with the highest rate of bone metastases, which can greatly reduce a patient's quality of life. In recent years, cell‑derived exosomes, which can contain a wide range of biologically active molecules, have been reported as a novel method of communication among individual cells. However, the specific role that exosomes serve in this disease has not been fully elucidated. The prostate cancer cell line PC‑3 were applied in the present study, where its exosomes were isolated to explore their potential effects on osteoclast differentiation. Exosomes are extracellular vesicles secreted by cells. The size of exosomes is 30‑150 nm. They have double membrane structure and saucer‑like morphology. They contain rich contents (including nucleic acid, protein and lipid) and participate in molecular transmission between cells. The combined results of tartrate‑resistant acid phosphatase staining (to identify osteoclasts obtained from human peripheral blood mononuclear cells), reverse transcription‑quantitative PCR and western blotting showed that PC‑3‑derived exosomes attenuated osteoclast differentiation by downregulating marker genes associated with osteoclastic maturation, including V‑maf musculoaponeurotic fibrosarcoma oncogene homolog B, matrix metalloproteinase 9 and integrin β3. microRNA (miR)‑148a expression was also found to be downregulated in osteoclasts by PC‑3‑derived exosomes. In addition, the mTOR and AKT signaling pathways were blocked after exposure to these PC‑3 cell‑derived exosomes. Therefore, results from the present study suggest that miR‑148a mimics may be a new therapeutic approach for the prevention of prostate cancer bone metastases.
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Copy and paste a formatted citation
Spandidos Publications style
Tian G, Hu K, Qiu S, Xie Y, Cao Y, Ni S and Zhang L: Exosomes derived from PC‑3 cells suppress osteoclast differentiation by downregulating miR‑148a and blocking the PI3K/AKT/mTOR pathway. Exp Ther Med 22: 1304, 2021.
APA
Tian, G., Hu, K., Qiu, S., Xie, Y., Cao, Y., Ni, S., & Zhang, L. (2021). Exosomes derived from PC‑3 cells suppress osteoclast differentiation by downregulating miR‑148a and blocking the PI3K/AKT/mTOR pathway. Experimental and Therapeutic Medicine, 22, 1304. https://doi.org/10.3892/etm.2021.10739
MLA
Tian, G., Hu, K., Qiu, S., Xie, Y., Cao, Y., Ni, S., Zhang, L."Exosomes derived from PC‑3 cells suppress osteoclast differentiation by downregulating miR‑148a and blocking the PI3K/AKT/mTOR pathway". Experimental and Therapeutic Medicine 22.5 (2021): 1304.
Chicago
Tian, G., Hu, K., Qiu, S., Xie, Y., Cao, Y., Ni, S., Zhang, L."Exosomes derived from PC‑3 cells suppress osteoclast differentiation by downregulating miR‑148a and blocking the PI3K/AKT/mTOR pathway". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1304. https://doi.org/10.3892/etm.2021.10739
Copy and paste a formatted citation
x
Spandidos Publications style
Tian G, Hu K, Qiu S, Xie Y, Cao Y, Ni S and Zhang L: Exosomes derived from PC‑3 cells suppress osteoclast differentiation by downregulating miR‑148a and blocking the PI3K/AKT/mTOR pathway. Exp Ther Med 22: 1304, 2021.
APA
Tian, G., Hu, K., Qiu, S., Xie, Y., Cao, Y., Ni, S., & Zhang, L. (2021). Exosomes derived from PC‑3 cells suppress osteoclast differentiation by downregulating miR‑148a and blocking the PI3K/AKT/mTOR pathway. Experimental and Therapeutic Medicine, 22, 1304. https://doi.org/10.3892/etm.2021.10739
MLA
Tian, G., Hu, K., Qiu, S., Xie, Y., Cao, Y., Ni, S., Zhang, L."Exosomes derived from PC‑3 cells suppress osteoclast differentiation by downregulating miR‑148a and blocking the PI3K/AKT/mTOR pathway". Experimental and Therapeutic Medicine 22.5 (2021): 1304.
Chicago
Tian, G., Hu, K., Qiu, S., Xie, Y., Cao, Y., Ni, S., Zhang, L."Exosomes derived from PC‑3 cells suppress osteoclast differentiation by downregulating miR‑148a and blocking the PI3K/AKT/mTOR pathway". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1304. https://doi.org/10.3892/etm.2021.10739
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