Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2021 Volume 22 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2021 Volume 22 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Matrine ameliorates the inflammatory response and lipid metabolism in vascular smooth muscle cells through the NF‑κB pathway

  • Authors:
    • Guanglei Wang
    • Chengyu Ji
    • Chunling Wang
    • Zhonghui Liu
    • Aizhong Qu
    • Huaixin Wang
  • View Affiliations / Copyright

    Affiliations: Department of Emergency Medicine, Yidu Central Hospital of Weifang, Weifang, Shandong 262500, P.R. China, Department of Cardiology, Linqu People's Hospital, Weifang, Shandong 262600, P.R. China, Department of Stomatology, Linqu People's Hospital, Weifang, Shandong 262600, P.R. China, Hematology Department, Weifang Yidu Central Hospital, Weifang, Shandong 262500, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1309
    |
    Published online on: September 16, 2021
       https://doi.org/10.3892/etm.2021.10744
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Atherosclerosis is a chronic inflammatory disease associated with inflammatory responses and the uncontrolled proliferation and excessive apoptosis of vascular smooth muscle cells. However, the effects of matrine on the inflammatory response, abnormal lipid metabolism and cell proliferation and apoptosis marker proteins in human aortic vascular smooth muscle cells (HAVSMCs) have not been elucidated. Therefore, the present study aimed to investigate the effect of matrine on an in vitro model of atherosclerosis using HAVSMCs. The HAVSMCs were divided into normal, model and matrine groups. The model group was treated with oxidized low‑density lipoprotein (oxLDL), the matrine group was treated with oxLDL and matrine and the normal group was treated with physiological saline. Total cholesterol (TC), free cholesterol (FC) and cholesterol ester (CE) levels were measured in the cell supernatant. In addition, the relative mRNA levels of inflammatory factors were quantified using reverse transcription‑quantitative PCR, and the cell proliferation and apoptosis rates were evaluated using Cell Counting Kit‑8 and flow cytometry assays, respectively. The expression levels of proteins associated with proliferation and apoptosis were also determined using western blotting. The levels of TC, FC and CE and the mRNA levels of IL‑1β, IL‑6, and TNF‑α in the matrine group were lower than those in the model group, but higher than those in the normal group. After 48 and 96 h of treatment, the cell proliferation and apoptotic rates were lower in the matrine group compared with the model group. The relative expression levels of Ki‑67, proliferating cell nuclear antigen and Bax were decreased, while that of Bcl‑2 was increased in the matrine group compared with the model group. In addition, the relative protein expression of nuclear factor κB (NF‑κB) in the matrine group was lower than that in the model group, but higher than that in the normal group. In summary, matrine inhibited activation of the NF‑κB pathway and reduced cell proliferation and apoptosis in the oxLDL‑induced atherosclerosis model, and exhibited anti‑inflammatory effects. These results suggest that matrine attenuated abnormal biological reactions in HAVSMCs through the NF‑κB pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

View References

1 

Tabas I, García-Cardeña G and Owens GK: Recent insights into the cellular biology of atherosclerosis. J Cell Biol. 209:13–22. 2015.PubMed/NCBI View Article : Google Scholar

2 

Gisterå A and Hansson GK: The immunology of atherosclerosis. Nat Rev Nephrol. 13:368–380. 2017.PubMed/NCBI View Article : Google Scholar

3 

Feinberg MW and Moore KJ: MicroRNA regulation of atherosclerosis. Circ Res. 118:703–720. 2016.PubMed/NCBI View Article : Google Scholar

4 

Bäck M and Hansson GK: Anti-inflammatory therapies for atherosclerosis. Nat Rev Cardiol. 12:199–211. 2015.PubMed/NCBI View Article : Google Scholar

5 

Libby P and Everett BM: Novel antiatherosclerotic therapies. Arterioscler Thromb Vasc Biol. 39:538–545. 2019.PubMed/NCBI View Article : Google Scholar

6 

Zhu Y, Xian X, Wang Z, Bi Y, Chen Q, Han X, Tang D and Chen R: Research progress on the relationship between atherosclerosis and inflammation. Biomolecules. 8(80)2018.PubMed/NCBI View Article : Google Scholar

7 

Zhang MJ and Huang J: Recent research progress of anti-tumor mechnism matrine. Zhongguo Zhong Yao Za Zhi. 29:115–118. 2004.PubMed/NCBI(In Chinese).

8 

Liu Y, Xu Y, Ji W, Li X, Sun B, Gao Q and Su C: Anti-tumor activities of matrine and oxymatrine: Literature review. Tumour Biol. 35:5111–5119. 2014.PubMed/NCBI View Article : Google Scholar

9 

Suo Z, Liu Y, Ferreri M, Zhang T, Liu Z, Mu X and Han B: Impact of matrine on inflammation related factors in rat intestinal microvascular endothelial cells. J Ethnopharmacol. 125:404–409. 2009.PubMed/NCBI View Article : Google Scholar

10 

Yu HB, Zhang HF, Li DY, Zhang X, Xue HZ and Zhao SH: Matrine inhibits matrix metalloproteinase-9 expression and invasion of human hepatocellular carcinoma cells. J Asian Nat Prod Res. 13:242–250. 2011.PubMed/NCBI View Article : Google Scholar

11 

Liu XY, Fang H, Yang ZG, Wang XY, Ruan LM, Fang DR, Ding YG, Wang YN, Zhang Y, Jiang XL and Chen HC: Matrine inhibits invasiveness and metastasis of human malignant melanoma cell line A375 in vitro. Int J Dermatol. 47:448–456. 2008.PubMed/NCBI View Article : Google Scholar

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

13 

de Boer OJ, van der Wal AC and Becker AE: Atherosclerosis, inflammation, and infection. J Pathol. 190:237–243. 2000.PubMed/NCBI View Article : Google Scholar

14 

Libby P, Ridker PM and Hansson GK: Leducq Transatlantic Network on Atherothrombosis. Inflammation in atherosclerosis: From pathophysiology to practice. J Am Coll Cardiol. 54:2129–2138. 2009.PubMed/NCBI View Article : Google Scholar

15 

Faggioni R, Fantuzzi G, Fuller J, Dinarello CA, Feingold KR and Grunfeld C: IL-1beta mediates leptin induction during inflammation. Am J Physiol. 274:R204–R208. 1998.PubMed/NCBI View Article : Google Scholar

16 

Lopez-Castejon G and Brough D: Understanding the mechanism of IL-1β secretion. Cytokine Growth Factor Rev. 22:189–195. 2011.PubMed/NCBI View Article : Google Scholar

17 

Bhaskar V, Yin J, Mirza AM, Phan D, Vanegas S, Issafras H, Michelson K, Hunter JJ and Kantak SS: Monoclonal antibodies targeting IL-1 beta reduce biomarkers of atherosclerosis in vitro and inhibit atherosclerotic plaque formation in Apolipoprotein E-deficient mice. Atherosclerosis. 216:313–320. 2011.PubMed/NCBI View Article : Google Scholar

18 

Rus HG, Vlaicu R and Niculescu F: Interleukin-6 and interleukin-8 protein and gene expression in human arterial atherosclerotic wall. Atherosclerosis. 127:263–271. 1996.PubMed/NCBI View Article : Google Scholar

19 

Stenvinkel P, Barany P, Heimbürger O, Pecoits-Filho R and Lindholm B: Mortality, malnutrition, and atherosclerosis in ESRD: What is the role of interleukin-6? Kidney Int. Suppl:103–108. 2002.PubMed/NCBI View Article : Google Scholar

20 

Seino Y, Ikeda U, Ikeda M, Yamamoto K, Misawa Y, Hasegawa T, Kano S and Shimada K: Interleukin 6 gene transcripts are expressed in human atherosclerotic lesions. Cytokine. 6:87–91. 1994.PubMed/NCBI View Article : Google Scholar

21 

Skoog T, Dichtl W, Boquist S, Skoglund-Andersson C, Karpe F, Tang R, Bond MG, de Faire U, Nilsson J, Eriksson P and Hamsten A: Plasma tumour necrosis factor-alpha and early carotid atherosclerosis in healthy middle-aged men. Eur Heart J. 23:376–383. 2002.PubMed/NCBI View Article : Google Scholar

22 

Suarez EC, Lewis JG and Kuhn C: The relation of aggression, hostility, and anger to lipopolysaccharide-stimulated tumor necrosis factor (TNF)-alpha by blood monocytes from normal men. Brain Behav Immun. 16:675–684. 2002.PubMed/NCBI View Article : Google Scholar

23 

Zhou J, Ma W, Wang X, Liu H, Miao Y, Wang J, Du P, Chen Y, Zhang Y and Liu Z: Matrine suppresses reactive oxygen species (ROS)-mediated MKKs/p38-induced inflammation in oxidized low-density lipoprotein (ox-LDL)-stimulated macrophages. Med Sci Monit. 25:4130–4136. 2019.PubMed/NCBI View Article : Google Scholar

24 

Zhong W, Peng J, He H, Wu D, Han Z, Bi X and Dai Q: Ki-67 and PCNA expression in prostate cancer and benign prostatic hyperplasia. Clin Invest Med. 31:E8–E15. 2008.PubMed/NCBI View Article : Google Scholar

25 

Kayaselçuk F, Zorludemir S, Gümürdühü D, Zeren H and Erman T: PCNA and Ki-67 in central nervous system tumors: Correlation with the histological type and grade. J Neurooncol. 57:115–121. 2002.PubMed/NCBI View Article : Google Scholar

26 

Reed JC: Proapoptotic multidomain Bcl-2/Bax-family proteins: Mechanisms, physiological roles, and therapeutic opportunities. Cell Death Differ. 13:1378–1386. 2006.PubMed/NCBI View Article : Google Scholar

27 

Zhang LP, Jiang JK, Tam JW, Zhang Y, Liu XS, Xu XR, Liu BZ and He YJ: Effects of matrine on proliferation and differentiation in K-562 cells. Leuk Res. 25:793–800. 2001.PubMed/NCBI View Article : Google Scholar

28 

Lawrence T: The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol. 1(a001651)2009.PubMed/NCBI View Article : Google Scholar

29 

Moynagh PN: The NF-kappaB pathway. J Cell Sci. 118:4589–4592. 2005.PubMed/NCBI View Article : Google Scholar

30 

Liu J, Zhang L, Ren Y, Gao Y, Kang L and Lu S: Matrine inhibits the expression of adhesion molecules in activated vascular smooth muscle cells. Mol Med Rep. 13:2313–2319. 2016.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang G, Ji C, Wang C, Liu Z, Qu A and Wang H: Matrine ameliorates the inflammatory response and lipid metabolism in vascular smooth muscle cells through the NF‑κB pathway. Exp Ther Med 22: 1309, 2021.
APA
Wang, G., Ji, C., Wang, C., Liu, Z., Qu, A., & Wang, H. (2021). Matrine ameliorates the inflammatory response and lipid metabolism in vascular smooth muscle cells through the NF‑κB pathway. Experimental and Therapeutic Medicine, 22, 1309. https://doi.org/10.3892/etm.2021.10744
MLA
Wang, G., Ji, C., Wang, C., Liu, Z., Qu, A., Wang, H."Matrine ameliorates the inflammatory response and lipid metabolism in vascular smooth muscle cells through the NF‑κB pathway". Experimental and Therapeutic Medicine 22.5 (2021): 1309.
Chicago
Wang, G., Ji, C., Wang, C., Liu, Z., Qu, A., Wang, H."Matrine ameliorates the inflammatory response and lipid metabolism in vascular smooth muscle cells through the NF‑κB pathway". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1309. https://doi.org/10.3892/etm.2021.10744
Copy and paste a formatted citation
x
Spandidos Publications style
Wang G, Ji C, Wang C, Liu Z, Qu A and Wang H: Matrine ameliorates the inflammatory response and lipid metabolism in vascular smooth muscle cells through the NF‑κB pathway. Exp Ther Med 22: 1309, 2021.
APA
Wang, G., Ji, C., Wang, C., Liu, Z., Qu, A., & Wang, H. (2021). Matrine ameliorates the inflammatory response and lipid metabolism in vascular smooth muscle cells through the NF‑κB pathway. Experimental and Therapeutic Medicine, 22, 1309. https://doi.org/10.3892/etm.2021.10744
MLA
Wang, G., Ji, C., Wang, C., Liu, Z., Qu, A., Wang, H."Matrine ameliorates the inflammatory response and lipid metabolism in vascular smooth muscle cells through the NF‑κB pathway". Experimental and Therapeutic Medicine 22.5 (2021): 1309.
Chicago
Wang, G., Ji, C., Wang, C., Liu, Z., Qu, A., Wang, H."Matrine ameliorates the inflammatory response and lipid metabolism in vascular smooth muscle cells through the NF‑κB pathway". Experimental and Therapeutic Medicine 22, no. 5 (2021): 1309. https://doi.org/10.3892/etm.2021.10744
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team