Long non‑coding RNA RP11‑84C13.1 promotes osteogenic differentiation of bone mesenchymal stem cells and alleviates osteoporosis progression via the miR‑23b‑3p/RUNX2 axis

Yu,Huaixi; Li,Yunyun; Tang,Jinshan; Lu,Xiaoqing; Hu,Wen; Cheng,Liang

doi:10.3892/etm.2021.10775

Long non‑coding RNA RP11‑84C13.1 promotes osteogenic differentiation of bone mesenchymal stem cells and alleviates osteoporosis progression via the miR‑23b‑3p/RUNX2 axis

Authors:
- Huaixi Yu
- Yunyun Li
- Jinshan Tang
- Xiaoqing Lu
- Wen Hu
- Liang Cheng
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Affiliations: Department of Orthopaedic Surgery, Huai'an Second People's Hospital, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, Jiangsu 223000, P.R. China, Department of Information Statistics Center, Huai'an Second People's Hospital, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, Jiangsu 223000, P.R. China, Department of Endocrinology, Huai'an Second People's Hospital, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, Jiangsu 223000, P.R. China
Published online on: September 22, 2021 https://doi.org/10.3892/etm.2021.10775
Article Number: 1340
Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The objective of the present study was to determine the role of RP11‑84C13.1 in osteoporosis (OP) and its molecular mechanism. First, clinical samples were collected from OP patients and normal control patients. Human bone marrow stromal cells (hBMSCs) were extracted from femoral head tissues. Runt‑related transcription factor 2 (RUNX2) and RP11‑84C13.1 serum levels were assessed by reverse transcription‑quantitative (RT‑q)PCR. Following transfection of pcDNA‑RP11‑84C13.1, si‑RP11‑84C13.1, microRNA (miRNA)‑23b‑3p mimic and miRNA‑23b‑3p inhibitor, the expression levels of RUNX2 and RP11‑84C13.1 were determined by RT‑qPCR. In addition, the osteogenic ability of hBMSCs was assessed by Alizarin Red staining. The binding of RP11‑84C13.1 to miRNA‑23b‑3p and the binding of miRNA‑23b‑3p to RUNX2 was confirmed by dual‑luciferase reporter gene assay. Long non‑coding RNA (lncRNA) RP11‑84C13.1 was significantly downregulated in the serum of OP patients. The osteogenic differentiation‑related genes RUNX2 and RP11‑84C13.1 were markedly upregulated in a time‑dependent manner, while the miRNA‑23b‑3p level gradually decreased in hBMSCs with the prolongation of osteogenesis. RP11‑84C13.1 knockdown inhibited the osteogenic differentiation of hBMSCs. Furthermore, RP11‑84C13.1 regulated RUNX2 expression by targeting miRNA‑23b‑3p. Overexpression of miRNA‑23b‑3p partially reversed the promoting effect of RP11‑84C13.1 on the osteogenesis of hBMSCs. In conclusion, lncRNA RP11‑84C13.1 upregulated RUNX2 by absorbing miRNA‑23b‑3p, and thus induced hBMSC osteogenesis to alleviate osteoporosis.

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