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MicroRNA‑218 aggravates H2O2‑induced damage in PC12 cells via spred2‑mediated autophagy

  • Authors:
    • Duoping Chen
    • Chunmei Li
    • Rui Lv
  • View Affiliations / Copyright

    Affiliations: Second Department of Orthopaedics, Zhang Ye People's Hospital Affiliated to Hexi University, Zhangye, Gansu 734000, P.R. China, Image Center, Zhang Ye People's Hospital Affiliated to Hexi University, Zhangye, Gansu 734000, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1352
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    Published online on: September 23, 2021
       https://doi.org/10.3892/etm.2021.10787
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Abstract

The current study aimed to investigate the effects and underlying mechanism of miR‑218 in H2O2‑induced neuronal injury. The impacts of miR‑218 knockdown on cell viability, apoptosis and autophagy‑associated proteins were detected by Cell Counting Kit‑8 assay, flow cytometry and western blotting in H2O2‑injured PC12 cells, respectively. Reverse transcription‑quantitative PCR (RT‑qPCR) and western blotting was executed to explore the expression level of miR‑218 and sprouty‑related EVH1 domainprotein2 (spred2) in H2O2‑stimulated cells. Besides, the regulatory association between miR‑218 and spred2 was explored through bioinformatics and luciferase reporter assay. Following knockdown of miR‑218 and spred2, the functions of miR‑218 and spred2 in H2O2‑injured cells were further studied. High expression level of miR‑218 was observed in H2O2‑disposed PC12 cells, while spred2 expression level was downregulated. Knockdown of miR‑218 expression alleviated H2O2-induced PC12 cell injury by increasing cell proliferation, and decreasing apoptosis and autophagy. Furthermore, spred2 was identified as a direct target of miR-218 and was negatively regulated by miR-218. Moreover, suppression of spred2 abrogated the protective effects of miR‑218 inhibition on H2O2‑injured PC12 cells. Depletion of miR‑218 protected PC12 cells against H2O2‑induced cell injury via the upregulation of spred2, which provided a promising therapeutic strategy for spinal cord injury.
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Copy and paste a formatted citation
Spandidos Publications style
Chen D, Li C and Lv R: MicroRNA‑218 aggravates H<sub>2</sub>O<sub>2</sub>‑induced damage in PC12 cells via spred2‑mediated autophagy. Exp Ther Med 22: 1352, 2021.
APA
Chen, D., Li, C., & Lv, R. (2021). MicroRNA‑218 aggravates H<sub>2</sub>O<sub>2</sub>‑induced damage in PC12 cells via spred2‑mediated autophagy. Experimental and Therapeutic Medicine, 22, 1352. https://doi.org/10.3892/etm.2021.10787
MLA
Chen, D., Li, C., Lv, R."MicroRNA‑218 aggravates H<sub>2</sub>O<sub>2</sub>‑induced damage in PC12 cells via spred2‑mediated autophagy". Experimental and Therapeutic Medicine 22.6 (2021): 1352.
Chicago
Chen, D., Li, C., Lv, R."MicroRNA‑218 aggravates H<sub>2</sub>O<sub>2</sub>‑induced damage in PC12 cells via spred2‑mediated autophagy". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1352. https://doi.org/10.3892/etm.2021.10787
Copy and paste a formatted citation
x
Spandidos Publications style
Chen D, Li C and Lv R: MicroRNA‑218 aggravates H<sub>2</sub>O<sub>2</sub>‑induced damage in PC12 cells via spred2‑mediated autophagy. Exp Ther Med 22: 1352, 2021.
APA
Chen, D., Li, C., & Lv, R. (2021). MicroRNA‑218 aggravates H<sub>2</sub>O<sub>2</sub>‑induced damage in PC12 cells via spred2‑mediated autophagy. Experimental and Therapeutic Medicine, 22, 1352. https://doi.org/10.3892/etm.2021.10787
MLA
Chen, D., Li, C., Lv, R."MicroRNA‑218 aggravates H<sub>2</sub>O<sub>2</sub>‑induced damage in PC12 cells via spred2‑mediated autophagy". Experimental and Therapeutic Medicine 22.6 (2021): 1352.
Chicago
Chen, D., Li, C., Lv, R."MicroRNA‑218 aggravates H<sub>2</sub>O<sub>2</sub>‑induced damage in PC12 cells via spred2‑mediated autophagy". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1352. https://doi.org/10.3892/etm.2021.10787
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