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CMPK2 accelerates liver ischemia/reperfusion injury via the NLRP3 signaling pathway

  • Authors:
    • Yunhai Luo
    • Daofeng Zheng
    • Tong Mou
    • Junliang Pu
    • Zuotian Huang
    • Wei Chen
    • Yuke Zhang
    • Zhongjun Wu
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China
    Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1358
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    Published online on: September 24, 2021
       https://doi.org/10.3892/etm.2021.10793
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Abstract

Cytidine monophosphate kinase 2 (CMPK2) is a mitochondrial nucleotide monophosphate kinase which is important for the substrates of mitochondrial DNA synthesis and has been reported to participate in macrophage activation and the inflammatory response. The purpose of the present research was to determine the potential role of CMPK2 in hepatic ischemia/reperfusion (I/R) injury and to elucidate the underlying molecular mechanisms. The present study investigated the role of CMPK2 in regulating the NLRP3 pathway and liver dysfunction induced by hepatic I/R both in vivo and in vitro. It was revealed that hypoxia/reoxygenation (H/R) treatment enhanced the mRNA expression levels of CMPK2, NLRP3, IL‑18, IL‑1β and TNF‑α in RAW 264.7 cells. The protein expression levels of IL‑18, IL‑1β and cleaved‑caspase‑1 were decreased following CMPK2 knockdown. Furthermore, the inhibition of AIM2 downregulated the expression level of IL‑1β, IL‑18 and cleaved‑caspase‑1 in the CMPK2 knockdown group followed by H/R treatment, while the inhibition of NLRP3 did not. CMPK2 deficiency also decreased alanine aminotransferase and aspartate aminotransferase expression in mice serum, as well as the pathological changes in the liver. Similarly, the release of IL‑18 and IL‑1β in mouse serum was also restrained with the decline of CMPK2. In conclusion, the results of the present study demonstrate that CMPK2 is indispensable for NLRP3 inflammasome activation, making CMPK2 an effective target to relieve the liver from I/R injury. In addition, the function of CMPK2 is closely associated with NLRP3 inflammasome activation, instead of AIM2.
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Copy and paste a formatted citation
Spandidos Publications style
Luo Y, Zheng D, Mou T, Pu J, Huang Z, Chen W, Zhang Y and Wu Z: CMPK2 accelerates liver ischemia/reperfusion injury via the NLRP3 signaling pathway. Exp Ther Med 22: 1358, 2021.
APA
Luo, Y., Zheng, D., Mou, T., Pu, J., Huang, Z., Chen, W. ... Wu, Z. (2021). CMPK2 accelerates liver ischemia/reperfusion injury via the NLRP3 signaling pathway. Experimental and Therapeutic Medicine, 22, 1358. https://doi.org/10.3892/etm.2021.10793
MLA
Luo, Y., Zheng, D., Mou, T., Pu, J., Huang, Z., Chen, W., Zhang, Y., Wu, Z."CMPK2 accelerates liver ischemia/reperfusion injury via the NLRP3 signaling pathway". Experimental and Therapeutic Medicine 22.6 (2021): 1358.
Chicago
Luo, Y., Zheng, D., Mou, T., Pu, J., Huang, Z., Chen, W., Zhang, Y., Wu, Z."CMPK2 accelerates liver ischemia/reperfusion injury via the NLRP3 signaling pathway". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1358. https://doi.org/10.3892/etm.2021.10793
Copy and paste a formatted citation
x
Spandidos Publications style
Luo Y, Zheng D, Mou T, Pu J, Huang Z, Chen W, Zhang Y and Wu Z: CMPK2 accelerates liver ischemia/reperfusion injury via the NLRP3 signaling pathway. Exp Ther Med 22: 1358, 2021.
APA
Luo, Y., Zheng, D., Mou, T., Pu, J., Huang, Z., Chen, W. ... Wu, Z. (2021). CMPK2 accelerates liver ischemia/reperfusion injury via the NLRP3 signaling pathway. Experimental and Therapeutic Medicine, 22, 1358. https://doi.org/10.3892/etm.2021.10793
MLA
Luo, Y., Zheng, D., Mou, T., Pu, J., Huang, Z., Chen, W., Zhang, Y., Wu, Z."CMPK2 accelerates liver ischemia/reperfusion injury via the NLRP3 signaling pathway". Experimental and Therapeutic Medicine 22.6 (2021): 1358.
Chicago
Luo, Y., Zheng, D., Mou, T., Pu, J., Huang, Z., Chen, W., Zhang, Y., Wu, Z."CMPK2 accelerates liver ischemia/reperfusion injury via the NLRP3 signaling pathway". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1358. https://doi.org/10.3892/etm.2021.10793
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