Open Access

Protein tyrosine kinase 7‑knockdown inhibits oral squamous cell carcinoma cell viability, proliferation, migration and invasion via downregulating dishevelled segment polarity protein 3 expression

  • Authors:
    • Xiaoye Jin
    • Tao Huang
    • Caihong Ma
    • Jiafeng Duan
    • Rong Li
    • Wei Zhang
    • Wenyan Tian
  • View Affiliations

  • Published online on: September 28, 2021     https://doi.org/10.3892/etm.2021.10806
  • Article Number: 1372
  • Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Protein tyrosine kinase 7 (PTK7) expression has been reported to be dysregulated and to regulate various cellular activities in numerous types of cancer. However, to the best of our knowledge, the status and role of PTK7 in oral squamous cell carcinoma (OSCC) remains largely unknown. The present study aimed to investigate the involvement of PTK7 in OSCC progression and to determine the potential underlying mechanisms of action. The expression levels of PTK7 and dishevelled segment polarity protein 3 (DVL3) in OSCC cell lines were analyzed using reverse transcription‑quantitative PCR and western blotting. A co‑immunoprecipitation assay was used to verify the binding association between PTK7 and DVL3. In addition, OSCC cells were transfected with a short hairpin RNA targeting PTK7 or pcDNA‑DVL3 overexpression vectors. The effect of PTK7 on OSCC cell viability, proliferation, migration and invasion, and the underlying mechanisms, were investigated using Cell Counting Kit‑8, colony formation, wound healing and Transwell assays, respectively. Western blotting was used to analyze the expression levels of proliferation‑ and migration‑associated proteins. The results revealed that the expression levels of both PTK7 and DVL3 were significantly upregulated in OSCC cell lines. In addition, a binding association was identified between PTK7 and DVL3 in SCC‑9 cells. The knockdown of PTK7 expression inhibited OSCC cell viability, proliferation, invasion and migration, while the overexpression of DVL3 reversed the inhibitory effects of PTK7‑knockdown on OSCC cells. In conclusion, the results of the present study suggested that PTK7 may be a key regulator of OSCC proliferation, migration and invasion, and PTK7‑knockdown may inhibit OSCC cell viability, proliferation, invasion and migration by downregulating DVL3 expression. Therefore, PTK7 and DVL3 may represent potential biomarkers for diagnosis and treatment, as well as promising drug targets for OSCC.
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December-2021
Volume 22 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Jin X, Huang T, Ma C, Duan J, Li R, Zhang W and Tian W: Protein tyrosine kinase 7‑knockdown inhibits oral squamous cell carcinoma cell viability, proliferation, migration and invasion via downregulating dishevelled segment polarity protein 3 expression. Exp Ther Med 22: 1372, 2021.
APA
Jin, X., Huang, T., Ma, C., Duan, J., Li, R., Zhang, W., & Tian, W. (2021). Protein tyrosine kinase 7‑knockdown inhibits oral squamous cell carcinoma cell viability, proliferation, migration and invasion via downregulating dishevelled segment polarity protein 3 expression. Experimental and Therapeutic Medicine, 22, 1372. https://doi.org/10.3892/etm.2021.10806
MLA
Jin, X., Huang, T., Ma, C., Duan, J., Li, R., Zhang, W., Tian, W."Protein tyrosine kinase 7‑knockdown inhibits oral squamous cell carcinoma cell viability, proliferation, migration and invasion via downregulating dishevelled segment polarity protein 3 expression". Experimental and Therapeutic Medicine 22.6 (2021): 1372.
Chicago
Jin, X., Huang, T., Ma, C., Duan, J., Li, R., Zhang, W., Tian, W."Protein tyrosine kinase 7‑knockdown inhibits oral squamous cell carcinoma cell viability, proliferation, migration and invasion via downregulating dishevelled segment polarity protein 3 expression". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1372. https://doi.org/10.3892/etm.2021.10806