Open Access

Baicalein pre‑treatment alleviates hepatic ischemia/reperfusion injury in mice by regulating the Nrf2/ARE pathway

  • Authors:
    • Yi Zhou
    • Zhi Tan
    • Hao Huang
    • Yonglian Zeng
    • Shilian Chen
    • Jie Wei
    • Guozhen Huang
    • Chaosi Qian
    • Guandou Yuan
    • Songqing He
  • View Affiliations

  • Published online on: September 28, 2021     https://doi.org/10.3892/etm.2021.10816
  • Article Number: 1380
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hepatic ischemia‑reperfusion injury (HIRI) is caused by blood flow recovery following ischemia. Baicalein (BAI), a natural antioxidant used in traditional Chinese medicine, eliminates excessive free radicals and protects the structure of the cell membrane. However, its protective mechanism against HIRI is still unclear. The present study investigated underlying mechanism using a mouse HIRI model. Liver injury was evaluated using serum levels of alanine aminotransferase and aspartate aminotransferase, and hematoxylin‑eosin staining was performed to evaluate the pathological changes in liver tissue. Apoptosis of hepatocytes was detected by TUNEL staining. The expression levels of reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) in the liver were detected to evaluate oxidative stress. Western blotting was performed to assess expression levels of nuclear factor E2‑related factor 2 (Nrf2)/antioxidant response elements (ARE) pathway proteins in liver tissue. BAI pre‑treatment significantly decreased elevation of serum aminotransferase levels induced by IR and alleviated histological damage to the liver. BAI decreased production of ROS and MDA in liver tissue induced by IR and increased the activity of SOD. At the same time, BAI inhibited apoptosis of liver cells induced by oxidative stress. Furthermore, BAI promoted the translocation of Nrf2 into the nucleus and increased the expression of total heme oxygenase‑1 and NAD(P)H dehydrogenase quinone‑1. The Nrf2 inhibitor ML385 reversed the protective effect of BAI on HIRI. These results indicated that BAI served a protective effect in HIRI by regulating the Nrf2/ARE pathway.
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December-2021
Volume 22 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhou Y, Tan Z, Huang H, Zeng Y, Chen S, Wei J, Huang G, Qian C, Yuan G, He S, He S, et al: Baicalein pre‑treatment alleviates hepatic ischemia/reperfusion injury in mice by regulating the Nrf2/ARE pathway. Exp Ther Med 22: 1380, 2021
APA
Zhou, Y., Tan, Z., Huang, H., Zeng, Y., Chen, S., Wei, J. ... He, S. (2021). Baicalein pre‑treatment alleviates hepatic ischemia/reperfusion injury in mice by regulating the Nrf2/ARE pathway. Experimental and Therapeutic Medicine, 22, 1380. https://doi.org/10.3892/etm.2021.10816
MLA
Zhou, Y., Tan, Z., Huang, H., Zeng, Y., Chen, S., Wei, J., Huang, G., Qian, C., Yuan, G., He, S."Baicalein pre‑treatment alleviates hepatic ischemia/reperfusion injury in mice by regulating the Nrf2/ARE pathway". Experimental and Therapeutic Medicine 22.6 (2021): 1380.
Chicago
Zhou, Y., Tan, Z., Huang, H., Zeng, Y., Chen, S., Wei, J., Huang, G., Qian, C., Yuan, G., He, S."Baicalein pre‑treatment alleviates hepatic ischemia/reperfusion injury in mice by regulating the Nrf2/ARE pathway". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1380. https://doi.org/10.3892/etm.2021.10816