Open Access

NEDD4 E3 ubiquitin protein ligase serves an important role in cutaneous melanoma occurrence and development

  • Authors:
    • Fang Cheng
    • Yi Cheng
    • Xiaoling Zhao
    • Lihui An
    • Linfang Yang
    • Zihan Li
    • Lei Zhang
    • Runzhi He
  • View Affiliations

  • Published online on: September 28, 2021     https://doi.org/10.3892/etm.2021.10818
  • Article Number: 1382
  • Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].

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Abstract

The present study aimed to discuss the effects and relative mechanisms of NEDD4 E3 ubiquitin protein ligase (NEDD4) in cutaneous melanoma (CMM) occurrence and development. Clinical cancer and adjacent normal tissues samples were collected to analyze pathological changes and protein expression of NEDD4. Moreover, small interfering (si)RNA was used to knockdown NEDD4 expression in SK‑MEL‑2 and Malme‑3M cells. Cellular proliferation, apoptosis, invasiveness and migration were examined using colony formation, flow cytometric, Transwell and wound‑healing assays, respectively. In addition, the relative mRNA and protein expression levels of NEDD4, notch receptor 1 (Notch1) and PTEN were evaluated via reverse transcription‑quantitative (RT‑q) PCR and western blotting. It was found that NEDD4 mRNA and protein expression were significantly upregulated (both P<0.01). Following NEDD4‑knockdown, colony number was significantly decreased, while the apoptotic rate was significantly increased, the invasive cell number was significantly inhibited and the wound‑healing capacity was significantly decreased. Following si‑NEDD4 transfection, RT‑qPCR and western blotting revealed that NEDD4 and Notch1 mRNA and protein expression levels were significantly downregulated, while those of PTEN were significantly upregulated in the SK‑MEL‑2 and Malme‑3M cell lines. Collectively, the current results suggest that NEDD4‑knockdown effectively suppressed CMM biological activity by regulating the Notch1/PTEN pathway in vitro.
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December-2021
Volume 22 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Cheng F, Cheng Y, Zhao X, An L, Yang L, Li Z, Zhang L and He R: NEDD4 E3 ubiquitin protein ligase serves an important role in cutaneous melanoma occurrence and development. Exp Ther Med 22: 1382, 2021
APA
Cheng, F., Cheng, Y., Zhao, X., An, L., Yang, L., Li, Z. ... He, R. (2021). NEDD4 E3 ubiquitin protein ligase serves an important role in cutaneous melanoma occurrence and development. Experimental and Therapeutic Medicine, 22, 1382. https://doi.org/10.3892/etm.2021.10818
MLA
Cheng, F., Cheng, Y., Zhao, X., An, L., Yang, L., Li, Z., Zhang, L., He, R."NEDD4 E3 ubiquitin protein ligase serves an important role in cutaneous melanoma occurrence and development". Experimental and Therapeutic Medicine 22.6 (2021): 1382.
Chicago
Cheng, F., Cheng, Y., Zhao, X., An, L., Yang, L., Li, Z., Zhang, L., He, R."NEDD4 E3 ubiquitin protein ligase serves an important role in cutaneous melanoma occurrence and development". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1382. https://doi.org/10.3892/etm.2021.10818