Open Access

DDX3X deficiency alleviates LPS‑induced H9c2 cardiomyocytes pyroptosis by suppressing activation of NLRP3 inflammasome

  • Authors:
    • Dandan Feng
    • Liang Guo
    • Jing Liu
    • Yunxuan Song
    • Xiuyuan Ma
    • Haiyang Hu
    • Ju Liu
    • Enkui Hao
  • View Affiliations

  • Published online on: September 30, 2021     https://doi.org/10.3892/etm.2021.10825
  • Article Number: 1389
  • Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Increasing evidence suggest that NOD‑like receptor protein 3 (NLRP3) inflammasome‑mediated pyroptosis may be the underlying pathological mechanism of sepsis‑induced cardiomyopathy. DDX3X, an ATP‑dependent RNA helicase, plays a vital role in the formation of the NLRP3 inflammasome by directly interacting with cytoplasmic NLRP3. However, whether DDX3X has a direct impact on lipopolysaccharide (LPS)‑induced cardiomyocyte injury by regulating NLRP3 inflammasome assembly remains unclear. The present study aimed to investigate the role of DDX3X in the activation of the NLRP3 inflammasome and determine the molecular mechanism of DDX3X action in LPS‑induced pyroptosis in H9c2 cardiomyocytes. H9c2 cardiomyocytes were treated with LPS to simulate sepsis in vitro. The results demonstrated that LPS stimulation upregulated DDX3X expression in H9c2 cardiomyocytes. Furthermore, Ddx3x knockdown significantly attenuated pyroptosis and cell injury in LPS‑treated H9c2 cells by suppressing NLRP3 inflammasome activation. Taken together, these results suggest that DDX3X is involved in LPS‑induced cardiomyocyte pyroptosis, and DDX3X deficiency mitigates cardiomyocyte damage induced by LPS treatment.
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December-2021
Volume 22 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Feng D, Guo L, Liu J, Song Y, Ma X, Hu H, Liu J and Hao E: DDX3X deficiency alleviates LPS‑induced H9c2 cardiomyocytes pyroptosis by suppressing activation of NLRP3 inflammasome. Exp Ther Med 22: 1389, 2021
APA
Feng, D., Guo, L., Liu, J., Song, Y., Ma, X., Hu, H. ... Hao, E. (2021). DDX3X deficiency alleviates LPS‑induced H9c2 cardiomyocytes pyroptosis by suppressing activation of NLRP3 inflammasome. Experimental and Therapeutic Medicine, 22, 1389. https://doi.org/10.3892/etm.2021.10825
MLA
Feng, D., Guo, L., Liu, J., Song, Y., Ma, X., Hu, H., Liu, J., Hao, E."DDX3X deficiency alleviates LPS‑induced H9c2 cardiomyocytes pyroptosis by suppressing activation of NLRP3 inflammasome". Experimental and Therapeutic Medicine 22.6 (2021): 1389.
Chicago
Feng, D., Guo, L., Liu, J., Song, Y., Ma, X., Hu, H., Liu, J., Hao, E."DDX3X deficiency alleviates LPS‑induced H9c2 cardiomyocytes pyroptosis by suppressing activation of NLRP3 inflammasome". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1389. https://doi.org/10.3892/etm.2021.10825