Open Access

Downregulation of inhibitor of apoptosis protein induces apoptosis and suppresses stemness maintenance in testicular teratoma

  • Authors:
    • Gang Li
    • Man Liao
    • Shuang Li
    • Jia You
    • Jun Wang
    • Wei Lei
    • Chunlei Yang
    • Haolun Xu
    • He Xiao
    • Haitao Chen
  • View Affiliations

  • Published online on: October 1, 2021     https://doi.org/10.3892/etm.2021.10835
  • Article Number: 1399
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Inhibitors of apoptosis (IAPs) are a family of cell death inhibitors found in viruses and metazoans that physically interact with a variety of pro‑apoptotic proteins and inhibit apoptosis induced by diverse stimuli. Melanoma IAP (ML‑IAP) is a potent anti‑apoptotic protein that is strongly upregulated in melanoma and confers protection against a variety of pro‑apoptotic stimuli. In the present study, it was revealed that ML‑IAP was expressed at high levels in testicular teratoma. Deletion and mutational analysis demonstrated that ML‑IAP silencing significantly decreased P19 cell proliferation while inducing cell cycle arrest and apoptosis. ML‑IAP knockdown significantly induced caspase‑3/8/9‑mediated apoptosis in P19 cells. In addition, metabolism and stemness maintenance in P19 cells were suppressed by ML‑IAP knockdown. These results indicated that ML‑IAP silencing is a powerful inducer of apoptosis mediated by cell death receptors and may function as a direct activator of downstream effector caspases.
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