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Article

Influence of extracellular nanovesicles derived from adipose‑derived stem cells on nucleus pulposus cell from patients with intervertebral disc degeneration

  • Authors:
    • Zhiqiang Zhang
    • Liang Zhang
    • Jiandong Yang
    • Jijun Huang
    • Jun Cai
    • Shengfei Zhang
    • Xinmin Feng
    • Qiang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Clinical Medical College of Yangzhou University, Orthopaedic Institute, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu 225001, P.R. China
  • Article Number: 1431
    |
    Published online on: October 11, 2021
       https://doi.org/10.3892/etm.2021.10866
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Abstract

An increasing number of individuals are suffering from lower back and neck pain caused by intervertebral disc degeneration each year. Although the application of mesenchymal stem cells (MSCs) has provided desirable results in the treatment of intervertebral disc degeneration, there are multiple risks associated with the directed application of MSCs. An increasing number of studies have suggested that stem cells, through the release of extracellular nanovesicles, have vital functions in tissue regeneration and repair with low risk. The present study investigated the effect of extracellular nanovesicles derived from adipose‑derived stem cells (ADSCs) on nucleus pulposus (NP) cells from patients with intervertebral disc degeneration. Human NP cells were obtained from patients with intervertebral disc degeneration undergoing surgical procedures in addition to ADSCs from liposuction patients. ADSC‑derived extracellular nanovesicles were isolated and characterized. The differentiation and biological activity of NP cells cultured with or without ADSC‑derived extracellular nanovesicles were assessed and inflammatory factors and intervertebral disc degeneration‑associated markers were also measured. The results indicated that extracellular nanovesicles derived from ADSCs increased the migration and proliferation of NP cells and inhibited inflammatory activity, suggesting their utility for the treatment of intervertebral disc degeneration.
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1 

Fernandez-Moure J, Moore CA, Kim K, Karim A, Smith K, Barbosa Z, Van Eps J, Rameshwar P and Weiner B: Novel therapeutic strategies for degenerative disc disease: Review of cell biology and intervertebral disc cell therapy. SAGE Open Med. 6(2050312118761674)2018.PubMed/NCBI View Article : Google Scholar

2 

Petit A and Roquelaure Y: Low back pain, intervertebral disc and occupational diseases. Int J Occup Saf Ergon. 21:15–19. 2015.PubMed/NCBI View Article : Google Scholar

3 

Steelman T, Lewandowski L, Helgeson M, Wilson K, Olsen C and Gwinn D: Population-based risk factors for the development of degenerative disc disease. Clin Spine Surg. 31:E409–E412. 2018.PubMed/NCBI View Article : Google Scholar

4 

Jakoi AM, Pannu G, D'Oro A, Buser Z, Pham MH, Patel NN, Hsieh PC, Liu JC, Acosta FL, Hah R and Wang JC: The clinical correlations between diabetes, cigarette smoking and obesity on intervertebral degenerative disc disease of the lumbar spine. Asian Spine J. 11:337–347. 2017.PubMed/NCBI View Article : Google Scholar

5 

Teraguchi M, Yoshimura N, Hashizume H, Muraki S, Yamada H, Minamide A, Oka H, Ishimoto Y, Nagata K, Kagotani R, et al: Prevalence and distribution of intervertebral disc degeneration over the entire spine in a population-based cohort: The wakayama spine study. Osteoarthritis Cartilage. 22:104–110. 2014.PubMed/NCBI View Article : Google Scholar

6 

Clouet J, Fusellier M, Camus A, Le Visage C and Guicheux J: Intervertebral disc regeneration: From cell therapy to the development of novel bioinspired endogenous repair strategies. Adv Drug Deliv Rev. 146:306–324. 2019.PubMed/NCBI View Article : Google Scholar

7 

Alvi MA, Kerezoudis P, Wahood W, Goyal A and Bydon M: Operative approaches for lumbar disc herniation: A systematic review and multiple treatment meta-analysis of conventional and minimally invasive surgeries. World Neurosurg. 114:391–407 e2. 2018.PubMed/NCBI View Article : Google Scholar

8 

Ayala-Cuellar AP, Kang JH, Jeung EB and Choi KC: Roles of mesenchymal stem cells in tissue regeneration and immunomodulation. Biomol Ther (Seoul). 27:25–33. 2019.PubMed/NCBI View Article : Google Scholar

9 

Richardson SM, Kalamegam G, Pushparaj PN, Matta C, Memic A, Khademhosseini A, Mobasheri R, Poletti FL, Hoyland JA and Mobasheri A: Mesenchymal stem cells in regenerative medicine: Focus on articular cartilage and intervertebral disc regeneration. Methods. 99:69–80. 2016.PubMed/NCBI View Article : Google Scholar

10 

Yang F, Leung VYL, Luk KDK, Chan D and Cheung KMC: Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells. Mol Ther. 17:1959–1966. 2009.PubMed/NCBI View Article : Google Scholar

11 

Centeno C, Markle J, Dodson E, Stemper I, Williams CJ, Hyzy M, Ichim T and Freeman M: Treatment of lumbar degenerative disc disease-associated radicular pain with culture-expanded autologous mesenchymal stem cells: A pilot study on safety and efficacy. J Transl Med. 15(197)2017.PubMed/NCBI View Article : Google Scholar

12 

Raik S, Kumar A and Bhattacharyya S: Insights into cell-free therapeutic approach: Role of stem cell ‘soup-ernatant’. Biotechnol Appl Biochem. 65:104–118. 2018.PubMed/NCBI View Article : Google Scholar

13 

Liu X, Yang Y, Li Y, Niu X, Zhao B, Wang Y, Bao C, Xie Z, Lin Q and Zhu L: Integration of stem cell-derived exosomes with in situ hydrogel glue as a promising tissue patch for articular cartilage regeneration. Nanoscale. 9:4430–4438. 2017.PubMed/NCBI View Article : Google Scholar

14 

Merino-Gonzalez C, Zuñiga FA, Escudero C, Ormazabal V, Reyes C, Nova-Lamperti E, Salomón C and Aguayo C: Mesenchymal stem cell-derived extracellular vesicles promote angiogenesis: Potencial clinical application. Front Physiol. 7(24)2016.PubMed/NCBI View Article : Google Scholar

15 

Bucan V, Vaslaitis D, Peck CT, Strauß S, Vogt PM and Radtke C: Effect of exosomes from rat adipose-derived mesenchymal stem cells on neurite outgrowth and sciatic nerve regeneration after crush injury. Mol Neurobiol. 56:1812–1824. 2019.PubMed/NCBI View Article : Google Scholar

16 

Zhu Y, Wu Y, Cheng J, Wang Q, Li Z, Wang Y, Wang D, Wang H, Zhang W, Ye J, et al: Pharmacological activation of TAZ enhances osteogenic differentiation and bone formation of adipose-derived stem cells. Stem Cell Res Ther. 9(53)2018.PubMed/NCBI View Article : Google Scholar

17 

Chen D, Xia D, Pan Z, Xu D, Zhou Y, Wu Y, Cai N, Tang Q, Wang C, Yan M, et al: Metformin protects against apoptosis and senescence in nucleus pulposus cells and ameliorates disc degeneration in vivo. Cell Death Dis. 7(e2441)2016.PubMed/NCBI View Article : Google Scholar

18 

Baglio SR, Rooijers K, Koppers-Lalic D, Verweij FJ, Lanzón MP, Zini N, Naaijkens B, Perut F, Niessen HWM, Baldini N and Pegtel DM: Human bone marrow- and adipose-mesenchymal stem cells secrete exosomes enriched in distinctive miRNA and tRNA species. Stem Cell Res Ther. 6(127)2015.PubMed/NCBI View Article : Google Scholar

19 

Qu Y, Zhang Q, Cai X, Li F, Ma Z, Xu M and Lu L: Exosomes derived from miR-181-5p-modified adipose-derived mesenchymal stem cells prevent liver fibrosis via autophagy activation. J Cell Mol Med. 21:2491–2502. 2017.PubMed/NCBI View Article : Google Scholar

20 

Zhang J, Guan J, Niu X, Hu G, Guo S, Li Q, Xie Z, Zhang C and Wang Y: Exosomes released from human induced pluripotent stem cells-derived MSCs facilitate cutaneous wound healing by promoting collagen synthesis and angiogenesis. J Transl Med. 13(49)2015.PubMed/NCBI View Article : Google Scholar

21 

Barra GB, Santa Rita TH, Almeida ALSC, Jácomo RH and Nery LFA: Serum has higher proportion of janus kinase 2 V617F mutation compared to paired EDTA-whole blood sample: A model for somatic mutation quantification using qPCR and the 2-∆∆Cq method. Diagnostics (Basel). 10(153)2020.PubMed/NCBI View Article : Google Scholar

22 

Chung SS, Adekoya D, Enenmoh I, Clarke O, Wang P, Sarkyssian M, Wu Y and Vadgama JV: Salinomycin abolished STAT3 and STAT1 interactions and reduced telomerase activity in colorectal cancer cells. Anticancer Res. 37:445–453. 2017.PubMed/NCBI View Article : Google Scholar

23 

Perez-Cruet M, Beeravolu N, McKee C, Brougham J, Khan I, Bakshi S and Chaudhry GR: Potential of human nucleus pulposus-like cells derived from umbilical cord to treat degenerative disc disease. Neurosurgery. 84:272–283. 2019.PubMed/NCBI View Article : Google Scholar

24 

Cao C, Zou J, Liu X, Shapiro A, Moral M, Luo Z, Shi Q, Liu J, Yang H and Ebraheim N: Bone marrow mesenchymal stem cells slow intervertebral disc degeneration through the NF-kB pathway. Spine J. 15:530–538. 2015.PubMed/NCBI View Article : Google Scholar

25 

Leung VY, Aladin DMK, Lv F, Tam V, Sun Y, Lau RYC, Hung SC, Ngan AHW, Tang B, Lim CT, et al: Mesenchymal stem cells reduce intervertebral disc fibrosis and facilitate repair. Stem Cells. 32:2164–2177. 2014.PubMed/NCBI View Article : Google Scholar

26 

Lai RC, Tan SS, The BJ, Sze SK, Arslan F, de Kleijn DP, Choo A and Lim SK: Proteolytic potential of the MSC exosome proteome: Implications for an exosome-mediated delivery of therapeutic proteasome. Int J Proteomics. 2012(971907)2012.PubMed/NCBI View Article : Google Scholar

27 

Vizoso FJ, Eiro N, Cid S, Schneider J and Perez-Fernandez R: Mesenchymal stem cell secretome: Toward cell-free therapeutic strategies in regenerative medicine. Int J Mol Sci. 18(1852)2017.PubMed/NCBI View Article : Google Scholar

28 

Guillaume O, Naqvi SM, Lennon K and Buckley CT: Enhancing cell migration in shape-memory alginate-collagen composite scaffolds: In vitro and ex vivo assessment for intervertebral disc repair. J Biomater Appl. 29:1230–1246. 2015.PubMed/NCBI View Article : Google Scholar

29 

Lu K, Li HY, Yang K, Wu JL, Cai XW, Zhou Y and Li CQ: Exosomes as potential alternatives to stem cell therapy for intervertebral disc degeneration: In-vitro study on exosomes in interaction of nucleus pulposus cells and bone marrow mesenchymal stem cells. Stem Cell Res Ther. 8(108)2017.PubMed/NCBI View Article : Google Scholar

30 

Kim JS, Kwon D, Cha BH, Moon BK, Jeong YG, Han IB, Park H and Lee SH: Restoration of chondrogenic properties of degenerative nucleus pulposus cells by repeated co-culture with adipose-derived stem cells. J Industrial Engineering Chem. 60:185–191. 2018.

31 

Dowdell J, Erwin M, Choma T, Vaccaro A, Iatridis J and Cho SK: Intervertebral disk degeneration and repair. Neurosurgery. 80 (Suppl 3):S46–S54. 2017.PubMed/NCBI View Article : Google Scholar

32 

Khan AN, Jacobsen HE, Khan J, Filippi CG, Levine M, Lehman RA Jr, Riew KD, Lenke LG and Chahine NO: Inflammatory biomarkers of low back pain and disc degeneration: A review. Ann N Y Acad Sci. 1410:68–84. 2017.PubMed/NCBI View Article : Google Scholar

33 

Risbud MV and Shapiro IM: Role of cytokines in intervertebral disc degeneration: Pain and disc content. Nat Rev Rheumatol. 10:44–56. 2014.PubMed/NCBI View Article : Google Scholar

34 

Lai RC, Yeo RW and Lim SK: Mesenchymal stem cell exosomes. Semin Cell Dev Biol. 40:82–88. 2015.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhang Z, Zhang L, Yang J, Huang J, Cai J, Zhang S, Feng X and Wang Q: Influence of extracellular nanovesicles derived from adipose‑derived stem cells on nucleus pulposus cell from patients with intervertebral disc degeneration. Exp Ther Med 22: 1431, 2021.
APA
Zhang, Z., Zhang, L., Yang, J., Huang, J., Cai, J., Zhang, S. ... Wang, Q. (2021). Influence of extracellular nanovesicles derived from adipose‑derived stem cells on nucleus pulposus cell from patients with intervertebral disc degeneration. Experimental and Therapeutic Medicine, 22, 1431. https://doi.org/10.3892/etm.2021.10866
MLA
Zhang, Z., Zhang, L., Yang, J., Huang, J., Cai, J., Zhang, S., Feng, X., Wang, Q."Influence of extracellular nanovesicles derived from adipose‑derived stem cells on nucleus pulposus cell from patients with intervertebral disc degeneration". Experimental and Therapeutic Medicine 22.6 (2021): 1431.
Chicago
Zhang, Z., Zhang, L., Yang, J., Huang, J., Cai, J., Zhang, S., Feng, X., Wang, Q."Influence of extracellular nanovesicles derived from adipose‑derived stem cells on nucleus pulposus cell from patients with intervertebral disc degeneration". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1431. https://doi.org/10.3892/etm.2021.10866
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z, Zhang L, Yang J, Huang J, Cai J, Zhang S, Feng X and Wang Q: Influence of extracellular nanovesicles derived from adipose‑derived stem cells on nucleus pulposus cell from patients with intervertebral disc degeneration. Exp Ther Med 22: 1431, 2021.
APA
Zhang, Z., Zhang, L., Yang, J., Huang, J., Cai, J., Zhang, S. ... Wang, Q. (2021). Influence of extracellular nanovesicles derived from adipose‑derived stem cells on nucleus pulposus cell from patients with intervertebral disc degeneration. Experimental and Therapeutic Medicine, 22, 1431. https://doi.org/10.3892/etm.2021.10866
MLA
Zhang, Z., Zhang, L., Yang, J., Huang, J., Cai, J., Zhang, S., Feng, X., Wang, Q."Influence of extracellular nanovesicles derived from adipose‑derived stem cells on nucleus pulposus cell from patients with intervertebral disc degeneration". Experimental and Therapeutic Medicine 22.6 (2021): 1431.
Chicago
Zhang, Z., Zhang, L., Yang, J., Huang, J., Cai, J., Zhang, S., Feng, X., Wang, Q."Influence of extracellular nanovesicles derived from adipose‑derived stem cells on nucleus pulposus cell from patients with intervertebral disc degeneration". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1431. https://doi.org/10.3892/etm.2021.10866
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