Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
December-2021 Volume 22 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2021 Volume 22 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

lncRNA RP11‑531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression

  • Authors:
    • Yilin Wu
    • Fen Cai
    • Yuanbin Lu
    • Yanwei Hu
    • Qian Wang
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
  • Article Number: 1439
    |
    Published online on: October 12, 2021
       https://doi.org/10.3892/etm.2021.10874
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

A complete understanding of the behavioral influence and phenotypic transition of vascular smooth muscle cells, as well as the effects of the characteristics of these cells on the physiological and pathological processes of atherosclerosis, is crucial if new therapeutic targets for atherosclerosis are to be identified. In the present study, the long non‑coding RNA RP11‑531A24.3 was identified to be expressed at low levels in plaque tissues through screening a microarray for differentially expressed genes. The functional experimental results suggested that RP11‑531A24.3 reduced the viability and inhibited the migration of human aortic vascular smooth muscle cells (HA‑VSMCs). RNA antisense purification‑mass spectrometry was used to identify the RNA‑binding proteins (RBPs) for RP11‑531A24.3. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that the pathway with the highest degree of association with RP11‑531A24.3 RBPs was related to cell migration. The reduced migration and viability mediated by RP11‑531A24.3 overexpression was more significantly suppressed after annexin 2 (ANXA2) depletion in RP11‑531A24.3‑overexpressing HA‑VSMCs. Culture of HA‑VSMCs under hypoxic conditions (1% O2) reduced the expression of RP11‑531A24.3, and enhanced the protein expression of ANXA2 and HIF‑1α, while knockdown of ANXA2 downregulated the protein expression of HIF‑1α. These results suggested that RP11‑531A24.3 regulated the proliferation and migration of HA‑VSMCs through ANXA2 expression, and hypoxia may be an external factor in the regulation of RP11‑531A24.3 and its downstream targets.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Roth GA, Johnson C, Abajobir A, Abd-Allah F, Abera SF, Abyu G, Ahmed M, Aksut B, Alam T, Alam K, et al: Global, Regional, and National Burden of Cardiovascular Diseases for 10 Causes, 1990 to 2015. J Am Coll Cardiol. 70:1–25. 2017.PubMed/NCBI View Article : Google Scholar

2 

Song P, Zha M, Yang X, Xu Y, Wang H, Fang Z, Yang X, Xia W and Zeng C: Socioeconomic and geographic variations in the prevalence, awareness, treatment and control of dyslipidemia in middle-aged and older Chinese. Atherosclerosis. 282:57–66. 2019.PubMed/NCBI View Article : Google Scholar

3 

Lusis AJ: Atherosclerosis. Nature. 407:233–241. 2000.PubMed/NCBI View Article : Google Scholar

4 

Bennett MR, Sinha S and Owens GK: Vascular Smooth Muscle Cells in Atherosclerosis. Circ Res. 118:692–702. 2016.PubMed/NCBI View Article : Google Scholar

5 

Basatemur GL, Jørgensen HF, Clarke MCH, Bennett MR and Mallat Z: Vascular smooth muscle cells in atherosclerosis. Nat Rev Cardiol. 16:727–744. 2019.PubMed/NCBI View Article : Google Scholar

6 

Williams KJ and Tabas I: The response-to-retention hypothesis of early atherogenesis. Arterioscler Thromb Vasc Biol. 15:551–561. 1995.PubMed/NCBI View Article : Google Scholar

7 

Tabas I, Williams KJ and Borén J: Subendothelial lipoprotein retention as the initiating process in atherosclerosis: Update and therapeutic implications. Circulation. 116:1832–1844. 2007.PubMed/NCBI View Article : Google Scholar

8 

Owens GK, Kumar MS and Wamhoff BR: Molecular regulation of vascular smooth muscle cell differentiation in development and disease. Physiol Rev. 84:767–801. 2004.PubMed/NCBI View Article : Google Scholar

9 

Ferns GAA and Heikal L: Hypoxia in Atherogenesis. Angiology. 68:472–493. 2017.PubMed/NCBI View Article : Google Scholar

10 

Jain T, Nikolopoulou EA, Xu Q and Qu A: Hypoxia inducible factor as a therapeutic target for atherosclerosis. Pharmacol Ther. 183:22–33. 2018.PubMed/NCBI View Article : Google Scholar

11 

Luque A, Turu M, Juan-Babot O, Cardona P, Font A, Carvajal A, Slevin M, Iborra E, Rubio F, Badimon L, et al: Overexpression of hypoxia/inflammatory markers in atherosclerotic carotid plaques. Front Biosci. 13:6483–6490. 2008.PubMed/NCBI View Article : Google Scholar

12 

Consortium EP: ENCODE Project Consortium. An integrated encyclopedia of DNA elements in the human genome. Nature. 489:57–74. 2012.PubMed/NCBI View Article : Google Scholar

13 

Mishra K and Kanduri C: Understanding Long Noncoding RNA and Chromatin Interactions: What We Know So Far. Noncoding RNA. 5(5)2019.PubMed/NCBI View Article : Google Scholar

14 

McDonel P and Guttman M: Approaches for Understanding the Mechanisms of Long Noncoding RNA Regulation of Gene Expression. Cold Spring Harb Perspect Biol. 11(11)2019.PubMed/NCBI View Article : Google Scholar

15 

Sauvageau M: Diverging RNPs: Toward Understanding lncRNA-Protein Interactions and Functions. Adv Exp Med Biol. 1203:285–312. 2019.PubMed/NCBI View Article : Google Scholar

16 

Leeper NJ and Maegdefessel L: Non-coding RNAs: Key regulators of smooth muscle cell fate in vascular disease. Cardiovasc Res. 114:611–621. 2018.PubMed/NCBI View Article : Google Scholar

17 

Kumar S, Williams D, Sur S, Wang JY and Jo H: Role of flow-sensitive microRNAs and long noncoding RNAs in vascular dysfunction and atherosclerosis. Vascul Pharmacol. 114:76–92. 2019.PubMed/NCBI View Article : Google Scholar

18 

Holdt LM, Kohlmaier A and Teupser D: Long Noncoding RNAs of the Arterial Wall as Therapeutic Agents and Targets in Atherosclerosis. Thromb Haemost. 119:1222–1236. 2019.PubMed/NCBI View Article : Google Scholar

19 

Xu S, Kamato D, Little PJ, Nakagawa S, Pelisek J and Jin ZG: Targeting epigenetics and non-coding RNAs in atherosclerosis: From mechanisms to therapeutics. Pharmacol Ther. 196:15–43. 2019.PubMed/NCBI View Article : Google Scholar

20 

Hu YW, Guo FX, Xu YJ, Li P, Lu ZF, McVey DG, Zheng L, Wang Q, Ye JH, Kang CM, et al: Long noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN. J Clin Invest. 129:1115–1128. 2019.PubMed/NCBI View Article : Google Scholar

21 

Bai HL, Lu ZF, Zhao JJ, Ma X, Li XH, Xu H, Wu SG, Kang CM, Lu JB, Xu YJ, et al: Microarray profiling analysis and validation of novel long noncoding RNAs and mRNAs as potential biomarkers and their functions in atherosclerosis. Physiol Genomics. 51:644–656. 2019.PubMed/NCBI View Article : Google Scholar

22 

Kanehisa M, Furumichi M, Sato Y, Ishiguro-Watanabe M and Tanabe M: KEGG: Integrating viruses and cellular organisms. Nucleic Acids Res. 49D:D545–D551. 2021.PubMed/NCBI View Article : Google Scholar

23 

Gene Ontology C: Gene Ontology Consortium. The Gene Ontology resource: Enriching a GOld mine. Nucleic Acids Res. 49D:D325–D334. 2021.PubMed/NCBI View Article : Google Scholar

24 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

25 

Nan A, Chen L, Zhang N, Liu Z, Yang T, Wang Z, Yang C and Jiang Y: A novel regulatory network among lncRpa, circRar1, miR-671 and apoptotic genes promotes lead-induced neuronal cell apoptosis. Arch Toxicol. 91:1671–1684. 2017.PubMed/NCBI View Article : Google Scholar

26 

Fukata Y, Amano M and Kaibuchi K: Rho-Rho-kinase pathway in smooth muscle contraction and cytoskeletal reorganization of non-muscle cells. Trends Pharmacol Sci. 22:32–39. 2001.PubMed/NCBI View Article : Google Scholar

27 

Webb RC: Smooth muscle contraction and relaxation. Adv Physiol Educ. 27:201–206. 2003.PubMed/NCBI View Article : Google Scholar

28 

Lokman NA, Ween MP, Oehler MK and Ricciardelli C: The role of annexin A2 in tumorigenesis and cancer progression. Cancer Microenviron. 4:199–208. 2011.PubMed/NCBI View Article : Google Scholar

29 

Mayer G, Poirier S and Seidah NG: Annexin A2 is a C-terminal PCSK9-binding protein that regulates endogenous low density lipoprotein receptor levels. J Biol Chem. 283:31791–31801. 2008.PubMed/NCBI View Article : Google Scholar

30 

Ma S, Lu CC, Yang LY, Wang JJ, Wang BS, Cai HQ, Hao JJ, Xu X, Cai Y, Zhang Y, et al: ANXA2 promotes esophageal cancer progression by activating MYC-HIF1A-VEGF axis. J Exp Clin Cancer Res. 37(183)2018.PubMed/NCBI View Article : Google Scholar

31 

Hussain S, Saxena S, Shrivastava S, Mohanty AK, Kumar S, Singh RJ, Kumar A, Wani SA, Gandham RK, Kumar N, et al: Gene expression profiling of spontaneously occurring canine mammary tumours: Insight into gene networks and pathways linked to cancer pathogenesis. PLoS One. 13(e0208656)2018.PubMed/NCBI View Article : Google Scholar

32 

Tang Y, He Y, Zhang P, Wang J, Fan C, Yang L, Xiong F, Zhang S, Gong Z, Nie S, et al: lncRNAs regulate the cytoskeleton and related Rho/ROCK signaling in cancer metastasis. Mol Cancer. 17(77)2018.PubMed/NCBI View Article : Google Scholar

33 

Saiki Y and Horii A: Multiple functions of S100A10, an important cancer promoter. Pathol Int. 69:629–636. 2019.PubMed/NCBI View Article : Google Scholar

34 

Wang Z, Wei Q, Han L, Cao K, Lan T, Xu Z, Wang Y, Gao Y, Xue J, Shan F, et al: Tenascin-c renders a proangiogenic phenotype in macrophage via annexin II. J Cell Mol Med. 22:429–438. 2018.PubMed/NCBI View Article : Google Scholar

35 

Ding P, Ding Y, Tian Y and Lei X: Circular RNA circ_0010283 regulates the viability and migration of oxidized low density lipoprotein induced vascular smooth muscle cells via an miR 370 3p/HMGB1 axis in atherosclerosis. Int J Mol Med. 46:1399–1408. 2020.PubMed/NCBI View Article : Google Scholar

36 

Yan Z, Wang H, Liang J, Li Y and Li X: MicroRNA-503-5p improves carotid artery stenosis by inhibiting the proliferation of vascular smooth muscle cells. Exp Ther Med. 20(85)2020.PubMed/NCBI View Article : Google Scholar

37 

Deryugina EI and Quigley JP: Cell surface remodeling by plasmin: A new function for an old enzyme. J Biomed Biotechnol. 2012(564259)2012.PubMed/NCBI View Article : Google Scholar

38 

Li L, Li X, The E, Wang LJ, Yuan TY, Wang SY, Feng J, Wang J, Liu Y, Wu YH, et al: Low expression of lncRNA-GAS5 is implicated in human primary varicose great saphenous veins. PLoS One. 10(e0120550)2015.PubMed/NCBI View Article : Google Scholar

39 

Zhu B, Gong Y, Yan G, Wang D, Qiao Y, Wang Q, Liu B, Hou J, Li R and Tang C: Down-regulation of lncRNA MEG3 promotes hypoxia-induced human pulmonary artery smooth muscle cell proliferation and migration via repressing PTEN by sponging miR-21. Biochem Biophys Res Commun. 495:2125–2132. 2018.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wu Y, Cai F, Lu Y, Hu Y and Wang Q: lncRNA RP11‑531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression. Exp Ther Med 22: 1439, 2021.
APA
Wu, Y., Cai, F., Lu, Y., Hu, Y., & Wang, Q. (2021). lncRNA RP11‑531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression. Experimental and Therapeutic Medicine, 22, 1439. https://doi.org/10.3892/etm.2021.10874
MLA
Wu, Y., Cai, F., Lu, Y., Hu, Y., Wang, Q."lncRNA RP11‑531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression". Experimental and Therapeutic Medicine 22.6 (2021): 1439.
Chicago
Wu, Y., Cai, F., Lu, Y., Hu, Y., Wang, Q."lncRNA RP11‑531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1439. https://doi.org/10.3892/etm.2021.10874
Copy and paste a formatted citation
x
Spandidos Publications style
Wu Y, Cai F, Lu Y, Hu Y and Wang Q: lncRNA RP11‑531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression. Exp Ther Med 22: 1439, 2021.
APA
Wu, Y., Cai, F., Lu, Y., Hu, Y., & Wang, Q. (2021). lncRNA RP11‑531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression. Experimental and Therapeutic Medicine, 22, 1439. https://doi.org/10.3892/etm.2021.10874
MLA
Wu, Y., Cai, F., Lu, Y., Hu, Y., Wang, Q."lncRNA RP11‑531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression". Experimental and Therapeutic Medicine 22.6 (2021): 1439.
Chicago
Wu, Y., Cai, F., Lu, Y., Hu, Y., Wang, Q."lncRNA RP11‑531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1439. https://doi.org/10.3892/etm.2021.10874
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team