MicroRNA‑424 alleviates neurocyte injury by targeting PDCD4 in a cellular model of cerebral ischemic stroke

Ren,Hou-Wei; Gu,Bin; Zhang,Yue-Zhan; Guo,Ting; Wang,Qian; Shen,Yue-Qin; Wang,Jun

doi:10.3892/etm.2021.10888

MicroRNA‑424 alleviates neurocyte injury by targeting PDCD4 in a cellular model of cerebral ischemic stroke

Authors:
- Hou-Wei Ren
- Bin Gu
- Yue-Zhan Zhang
- Ting Guo
- Qian Wang
- Yue-Qin Shen
- Jun Wang
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Affiliations: Department of Emergency, Taizhou People's Hospital Affiliated to Nantong University, Taizhou, Jiangsu 225300, P.R. China, Department of Emergency, The Fourth Affiliated Hospital of Zhejiang University, School of Medicine, Yiwu, Zhejiang 322000, P.R. China
Published online on: October 15, 2021 https://doi.org/10.3892/etm.2021.10888
Article Number: 1453
Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cerebral ischemic stroke is the primary cause of stroke‑associated mortality and disability, and current therapeutic options are limited and ineffective. The present study aimed to investigate the potential of apoptotic therapy and the role of microRNA (miR)‑424 in cerebral ischemic stroke. PC12 cells, a cloned cell line from rat adrenal pheochromocytoma, were treated with CoCl2 to construct a cellular ischemia model. mRNA and protein levels of programmed cell death protein 4 (PDCD4), Bcl‑2, Bax, caspase‑3, PI3K and AKT were evaluated using reverse transcription‑quantitative PCR and western blot analyses, respectively. Cell Counting Kit‑8 assays were performed to examine cell viability in the ischemia model. Flow cytometry was conducted to evaluate the apoptosis of ischemic cells. Furthermore, a luciferase assay was performed to verify the target gene of miR‑424. It was revealed that the expression level of miR‑424 was downregulated in the ischemia model, while the expression of PDCD4 was upregulated. Moreover, the expression of miR‑424 was increased after treatment with miR‑424 mimics. The mRNA and protein expression of PDCD4 was upregulated after transfection with pcDNA3.1‑PDCD4. PDCD4 was predicted and demonstrated to be a target of miR‑424. Notably, overexpression of miR‑424 increased cell viability and inhibited apoptosis in the ischemia model, which was reversed by co‑treatment with pcDNA3.1‑PDCD4. Furthermore, overexpression of miR‑424 regulated the expression of PDCD4, Bax, Bcl‑2, phosphorylated‑PI3K/AKT and caspase‑3, which was restored after co‑transfection with pcDNA3.1‑PDCD4. Collectively, the results indicated that miR‑424 regulated the progression of cerebral ischemic stroke in a cellular model by targeting PDCD4.

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