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Article

Reduced miR‑519d‑3p levels in the synovium and synovial fluid facilitate the progression of post‑traumatic osteoarthritis by targeting VEGF

  • Authors:
    • Jianlong Gao
    • Silong Xia
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Affiliated Jianhu Hospital of Nantong University, Yancheng, Jiangsu 224700, P.R. China
  • Article Number: 1478
    |
    Published online on: October 25, 2021
       https://doi.org/10.3892/etm.2021.10913
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Abstract

The present study aimed to investigate the expression and clinical significance of miR‑519d‑3p in patients with post‑traumatic osteoarthritis (PTOA). The levels of miR‑519d‑3p in the synovium and synovial fluid (SF) of all subjects were detected by reverse transcription‑quantitative polymerase chain reaction. The results of the present study demonstrated that the levels of miR‑519d‑3p in the synovium and SF of patients with PTOA were significantly lower, but that the VEGF content was significantly higher, compared with that of control group. Dual‑luciferase reporter and Western blot assays demonstrated that VEGF was a target gene of miR‑519d‑3p. Furthermore, miR‑519d‑3p inhibitor‑induced cell apoptosis, and cell cycle arrest could be partially reversed by silencing VEGF. Additionally, the level of miR‑519d‑3p in the synovium and SF of patients with PTOA was negatively correlated with the level of VEGF. ROC analysis demonstrated that miR‑519d‑3p levels in the synovium and SF could effectively differentiate patients with PTOA from healthy controls, with areas under the ROC curve of 0.928 and 0.896, respectively. In conclusion, reduction of miR‑519d‑3p in the synovium and SF resulted in the upregulation of VEGF in patients with PTOA, and miR‑519d‑3p may be a potential therapeutic target of PTOA.
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1 

Takada S, Nakamura E, Sabanai K, Tsukamoto M, Otomo H, Kanoh S, Murai T, Fukuda H, Okada Y, Uchida S, et al: Attenuation of post-traumatic osteoarthritis after anterior cruciate ligament injury via inhibition of hedgehog signaling. J Orthop Res. 38:609–619. 2020.PubMed/NCBI View Article : Google Scholar

2 

Bodkin SG, Werner BC, Slater LV and Hart JM: Post-traumatic osteoarthritis diagnosed within 5 years following ACL reconstruction. Knee Surg Sports Traumatol Arthrosc. 28:790–796. 2020.PubMed/NCBI View Article : Google Scholar

3 

Brown SB, Hornyak JA, Jungels RR, Shah YY, Yarmola EG, Allen KD and Sharma B: Characterization of post-traumatic osteoarthritis in rats following anterior cruciate ligament rupture by non-invasive knee injury (NIKI). J Orthop Res. 38:356–367. 2020.PubMed/NCBI View Article : Google Scholar

4 

Cornelis FMF, de Roover A, Storms L, Hens A, Lories RJ and Monteagudo S: Increased susceptibility to develop spontaneous and post-traumatic osteoarthritis in Dot1l-deficient mice. Osteoarthritis Cartilage. 27:513–525. 2019.PubMed/NCBI View Article : Google Scholar

5 

Havryliuk H and Khimion L: Platelet autologous plasma in post-traumatic knee osteoarthritis treatment. J Clin Orthop Trauma. 10:42–45. 2019.PubMed/NCBI View Article : Google Scholar

6 

Rhon DI, Perez KG and Eskridge SL: Risk of post-traumatic knee osteoarthritis after knee injury in military service members. Musculoskelet Care. 17:113–119. 2019.PubMed/NCBI View Article : Google Scholar

7 

Lai Z and Cao Y: Plasma miR-200c-3p, miR-100-5p, and miR-1826 serve as potential diagnostic biomarkers for knee osteoarthritis: Randomized controlled trials. Medicine (Baltimore). 98(e18110)2019.PubMed/NCBI View Article : Google Scholar

8 

Ma F, Li G, Yu Y, Xu J and Wu X: miR-33b-3p promotes chondrocyte proliferation and inhibits chondrocyte apoptosis and cartilage ECM degradation by targeting DNMT3A in osteoarthritis. Biochem Biophys Res Commun. 519:430–437. 2019.PubMed/NCBI View Article : Google Scholar

9 

Ma Y, Wu Y, Chen J, Huang K, Ji B, Chen Z, Wang Q, Ma J, Shen S and Zhang J: miR-10a-5p promotes chondrocyte apoptosis in osteoarthritis by targeting HOXA1. Mol Ther Nucleic Acids. 14:398–409. 2019.PubMed/NCBI View Article : Google Scholar

10 

Li L, Jia J, Liu X, Yang S, Ye S, Yang W and Zhang Y: MicroRNA-16-5p controls development of osteoarthritis by targeting SMAD3 in chondrocytes. Curr Pharm Des. 21:5160–5167. 2015.PubMed/NCBI View Article : Google Scholar

11 

Zhao Z, Dai XS, Wang ZY, Bao ZQ and Guan JZ: MicroRNA-26a reduces synovial inflammation and cartilage injury in osteoarthritis of knee joints through impairing the NF-κB signaling pathway. Biosci Rep. 39(39)2019.PubMed/NCBI View Article : Google Scholar

12 

Ren L, Li Y, Zhao Q, Fan L, Tan B, Zang A and Yang H: miR-519 regulates the proliferation of breast cancer cells via targeting human antigen R. Oncol Lett. 19:1567–1576. 2020.PubMed/NCBI View Article : Google Scholar

13 

Xu J, You Q, Wei Z, Fu H, Zhang Y, Hu Z and Cai Q: miR-519 inhibits epithelial-mesenchymal transition and biologic behavior of gastric cancer cells by down-regulating FOXQ1. Int J Clin Exp Pathol. 13:425–436. 2020.PubMed/NCBI

14 

Yu G, Zhang T, Jing Y, Bao Q, Tang Q and Zhang Y: miR-519 suppresses nasopharyngeal carcinoma cell proliferation by targeting oncogene URG4/URGCP. Life Sci. 175:47–51. 2017.PubMed/NCBI View Article : Google Scholar

15 

Kellgren JH and Lawrence JS: Radiological assessment of rheumatoid arthritis. Ann Rheum Dis. 16:485–493. 1957.PubMed/NCBI View Article : Google Scholar

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

17 

Yang LH, Wang SL, Tang LL, Liu B, Ye WL, Wang LL, Wang ZY, Zhou MT and Chen BC: Universal stem-loop primer method for screening and quantification of microRNA. PLoS One. 9(e115293)2014.PubMed/NCBI View Article : Google Scholar

18 

Fang W, Guo J, Cao Y, Wang S, Pang C, Li M, Dou L, Man Y, Huang X, Shen T, et al: MicroRNA-20a-5p contributes to hepatic glycogen synthesis through targeting p63 to regulate p53 and PTEN expression. J Cell Mol Med. 20:1467–1480. 2016.PubMed/NCBI View Article : Google Scholar

19 

Hamilton JL, Nagao M, Levine BR, Chen D, Olsen BR and Im HJ: Targeting VEGF and its receptors for the treatment of osteoarthritis and associated Pain. J Bone Miner Res. 31:911–924. 2016.PubMed/NCBI View Article : Google Scholar

20 

Carballo CB, Coelho TR, de Holanda Afonso RC, Faria JC, Alves T, Monte SM, Ventura Matioszek GM, Moura-Neto V and Brito JM: Osteoarthritic synovial fluid and TGF-β1 induce interleukin-18 in articular chondrocytes. Cartilage. 11:385–394. 2020.PubMed/NCBI View Article : Google Scholar

21 

Hoff P, Buttgereit F, Burmester GR, Jakstadt M, Gaber T, Andreas K, Matziolis G, Perka C and Röhner E: Osteoarthritis synovial fluid activates pro-inflammatory cytokines in primary human chondrocytes. Int Orthop. 37:145–151. 2013.PubMed/NCBI View Article : Google Scholar

22 

Pearson MJ, Herndler-Brandstetter D, Tariq MA, Nicholson TA, Philp AM, Smith HL, Davis ET, Jones SW and Lord JM: IL-6 secretion in osteoarthritis patients is mediated by chondrocyte-synovial fibroblast cross-talk and is enhanced by obesity. Sci Rep. 7(3451)2017.PubMed/NCBI View Article : Google Scholar

23 

Singleton Q, Bapat S and Fulzele S: Post-traumatic osteoarthritis (PTOA) animal model to understand pathophysiology of osteoarthritis. Ann Transl Med. 7 (Suppl 3)(S81)2019.PubMed/NCBI View Article : Google Scholar

24 

Zou YC, Li HH, Yang GG, Yin HD, Cai DZ and Liu G: Attenuated levels of ghrelin in synovial fluid is related to the disease severity of ankle post-traumatic osteoarthritis. Biofactors. 45:463–470. 2019.PubMed/NCBI View Article : Google Scholar

25 

van Meurs JB: Osteoarthritis year in review 2016: Genetics, genomics and epigenetics. Osteoarthritis Cartilage. 25:181–189. 2017.PubMed/NCBI View Article : Google Scholar

26 

Sondag GR, Mbimba TS, Moussa FM, Novak K, Yu B, Jaber FA, Abdelmagid SM, Geldenhuys WJ and Safadi FF: Osteoactivin inhibition of osteoclastogenesis is mediated through CD44-ERK signaling. Exp Mol Med. 48(e257)2016.PubMed/NCBI View Article : Google Scholar

27 

Abdelmohsen K, Srikantan S, Kuwano Y and Gorospe M: miR-519 reduces cell proliferation by lowering RNA-binding protein HuR levels. Proc Natl Acad Sci USA. 105:20297–20302. 2008.PubMed/NCBI View Article : Google Scholar

28 

Yuan Q, Sun L, Li JJ and An CH: Elevated VEGF levels contribute to the pathogenesis of osteoarthritis. BMC Musculoskelet Disord. 15(437)2014.PubMed/NCBI View Article : Google Scholar

29 

Nagao M, Hamilton JL, Kc R, Berendsen AD, Duan X, Cheong CW, Li X, Im HJ and Olsen BR: Vascular endothelial growth factor in cartilage development and osteoarthritis. Sci Rep. 7(13027)2017.PubMed/NCBI View Article : Google Scholar

30 

Wang WT, Huang ZP, Sui S, Liu JH, Yu DM and Wang WB: microRNA-1236 promotes chondrocyte apoptosis in osteoarthritis via direct suppression of PIK3R3. Life Sci. 253(117694)2020.PubMed/NCBI View Article : Google Scholar

31 

Feng L, Feng C, Wang CX, Xu DY, Chen JJ, Huang JF, Tan PL and Shen JM: Circulating microRNA let 7e is decreased in knee osteoarthritis, accompanied by elevated apoptosis and reduced autophagy. Int J Mol Med. 45:1464–1476. 2020.PubMed/NCBI View Article : Google Scholar

32 

Li GS, Cui L and Wang GD: miR-155-5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis. Exp Ther Med. 21(68)2021.PubMed/NCBI View Article : Google Scholar

33 

Li L, Lv G, Wang B and Kuang L: XIST/miR-376c-5p/OPN axis modulates the influence of proinflammatory M1 macrophages on osteoarthritis chondrocyte apoptosis. J Cell Physiol. 235:281–293. 2020.PubMed/NCBI View Article : Google Scholar

34 

Sun Y, Kang S, Pei S, Sang C and Huang Y: MiR93-5p inhibits chondrocyte apoptosis in osteoarthritis by targeting lncRNA CASC2. BMC Musculoskelet Disord. 21(26)2020.PubMed/NCBI View Article : Google Scholar

35 

Tian J, Cheng C, Kuang SD, Su C, Zhao X, Xiong YL, Li YS and Gao SG: OPN Deficiency increases the severity of osteoarthritis associated with aberrant chondrocyte senescence and apoptosis and upregulates the expression of osteoarthritis-associated genes. Pain Res Manag. 2020(3428587)2020.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Gao J and Xia S: Reduced miR‑519d‑3p levels in the synovium and synovial fluid facilitate the progression of post‑traumatic osteoarthritis by targeting VEGF. Exp Ther Med 22: 1478, 2021.
APA
Gao, J., & Xia, S. (2021). Reduced miR‑519d‑3p levels in the synovium and synovial fluid facilitate the progression of post‑traumatic osteoarthritis by targeting VEGF. Experimental and Therapeutic Medicine, 22, 1478. https://doi.org/10.3892/etm.2021.10913
MLA
Gao, J., Xia, S."Reduced miR‑519d‑3p levels in the synovium and synovial fluid facilitate the progression of post‑traumatic osteoarthritis by targeting VEGF". Experimental and Therapeutic Medicine 22.6 (2021): 1478.
Chicago
Gao, J., Xia, S."Reduced miR‑519d‑3p levels in the synovium and synovial fluid facilitate the progression of post‑traumatic osteoarthritis by targeting VEGF". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1478. https://doi.org/10.3892/etm.2021.10913
Copy and paste a formatted citation
x
Spandidos Publications style
Gao J and Xia S: Reduced miR‑519d‑3p levels in the synovium and synovial fluid facilitate the progression of post‑traumatic osteoarthritis by targeting VEGF. Exp Ther Med 22: 1478, 2021.
APA
Gao, J., & Xia, S. (2021). Reduced miR‑519d‑3p levels in the synovium and synovial fluid facilitate the progression of post‑traumatic osteoarthritis by targeting VEGF. Experimental and Therapeutic Medicine, 22, 1478. https://doi.org/10.3892/etm.2021.10913
MLA
Gao, J., Xia, S."Reduced miR‑519d‑3p levels in the synovium and synovial fluid facilitate the progression of post‑traumatic osteoarthritis by targeting VEGF". Experimental and Therapeutic Medicine 22.6 (2021): 1478.
Chicago
Gao, J., Xia, S."Reduced miR‑519d‑3p levels in the synovium and synovial fluid facilitate the progression of post‑traumatic osteoarthritis by targeting VEGF". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1478. https://doi.org/10.3892/etm.2021.10913
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