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Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling

  • Authors:
    • Qiang Lin
    • Chunli Long
    • Zhengang Wang
    • Ronghui Wang
    • Wei Shi
    • Jiwei Qiu
    • Junlin Mo
    • Yongxiang Xie
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530023, P.R. China, Department of Health Maintenance, Faculty of Preclinical Medicine, Guangxi University of Chinese Medicine, Nanning, Guangxi 530001, P.R. China, Department of Endocrinology, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530023, P.R. China, Department of Critical Care Medicine, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530023, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 3
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    Published online on: October 26, 2021
       https://doi.org/10.3892/etm.2021.10924
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Abstract

Renal interstitial fibrosis (RIF) is the final common outcome of numerous chronic kidney diseases, contributing to end‑stage renal disease. Hirudin, a thrombin inhibitor, has attracted increased attention as a potential treatment approach for renal fibrosis. The present study aimed to investigate the molecular mechanism underlying the effect of hirudin on fibrosis in renal proximal tubular epithelial cells. An in vivo mouse RIF model established using unilateral ureteral obstruction (UUO) and an in vitro of RIF using the renal tubular epithelial cell line HK‑2 treated with TGF‑β were used. Expressions of sphingosine‑1‑phosphate (S1P) receptors (S1PR)1‑4 and protease‑activated receptor 1 (PAR1) were measured by reverse transcription‑quantitative PCR and western blotting in mice with UUO and TGF‑β induced HK‑2 cells. Western blotting was used to detect the expression of N‑cadherin, Slug, E‑cadherin, Collagen IV, fibronectin, MMP9 and monocyte chemoattractant protein‑1. Immunofluorescence staining was conducted to measure α‑SMA level expression. The results demonstrated that the expression levels of S1PR1, S1PR2, S1PR3, S1PR4 and PAR1 were upregulated in both TGF‑β‑induced HK‑2 cells and renal tissues from mice with unilateral ureteral ligation. Notably, hirudin inhibited TGF‑β‑induced PAR1, S1PR2 and S1PR3 upregulation in both HK‑2 cells and renal tissues. Additionally, the inhibition of S1PR2 and S1PR3 resulted in PAR1 downregulation. Furthermore, treatment with S1P and PAR1 agonists abolished the effect of hirudin on the expression of EMT, fibrosis‑related proteins and monocyte chemoattractant protein 1. In conclusion, hirudin attenuated TGF‑β‑induced fibrosis in proximal renal tubular epithelial HK‑2 cells by inhibiting PAR1 expression via the S1P/S1PR2/S1PR3 signaling pathway. Therefore, hirudin may be considered as a promising therapeutic agent for RIF.
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1 

Martínez-Klimova E, Aparicio-Trejo OE, Tapia E and Pedraza-Chaverri J: Unilateral ureteral obstruction as a model to investigate fibrosis-attenuating treatments. Biomolecules. 9(9)2019.PubMed/NCBI View Article : Google Scholar

2 

Wen Y, Rudemiller NP, Zhang J, Jeffs AD, Griffiths R, Lu X, Ren J, Privratsky J and Crowley SD: Stimulating type 1 angiotensin receptors on T lymphocytes attenuates renal fibrosis. Am J Pathol. 189:981–988. 2019.PubMed/NCBI View Article : Google Scholar

3 

Li R, Guo Y, Zhang Y, Zhang X, Zhu L and Yan T: Salidroside ameliorates renal interstitial fibrosis by inhibiting the TLR4/NF-κB and MAPK signaling pathways. Int J Mol Sci. 20(1103)2019.PubMed/NCBI View Article : Google Scholar

4 

Lv W, Fan F, Wang Y, Gonzalez-Fernandez E, Wang C, Yang L, Booz GW and Roman RJ: Therapeutic potential of microRNAs for the treatment of renal fibrosis and CKD. Physiol Genomics. 50:20–34. 2018.PubMed/NCBI View Article : Google Scholar

5 

Nastase MV, Zeng-Brouwers J, Wygrecka M and Schaefer L: Targeting renal fibrosis: Mechanisms and drug delivery systems. Adv Drug Deliv Rev. 129:295–307. 2018.PubMed/NCBI View Article : Google Scholar

6 

Humphreys BD: Mechanisms of renal fibrosis. Annu Rev Physiol. 80:309–326. 2018.PubMed/NCBI View Article : Google Scholar

7 

Chen Y, Mu L, Xing L, Li S and Fu S: Rhein alleviates renal interstitial fibrosis by inhibiting tubular cell apoptosis in rats. Biol Res. 52(50)2019.PubMed/NCBI View Article : Google Scholar

8 

Nogueira A, Pires MJ and Oliveira PA: Pathophysiological mechanisms of renal fibrosis: A review of animal models and therapeutic strategies. In Vivo. 31:1–22. 2017.PubMed/NCBI View Article : Google Scholar

9 

Ma Z, Wei Q, Zhang M, Chen JK and Dong Z: Dicer deficiency in proximal tubules exacerbates renal injury and tubulointerstitial fibrosis and upregulates Smad2/3. Am J Physiol Renal Physiol. 315:F1822–F1832. 2018.PubMed/NCBI View Article : Google Scholar

10 

Forbes MS, Thornhill BA, Minor JJ, Gordon KA, Galarreta CI and Chevalier RL: Fight-or-flight: Murine unilateral ureteral obstruction causes extensive proximal tubular degeneration, collecting duct dilatation, and minimal fibrosis. Am J Physiol Renal Physiol. 303:F120–F129. 2012.PubMed/NCBI View Article : Google Scholar

11 

Isaka Y: Targeting TGF-β signaling in kidney fibrosis. Int J Mol Sci. 19(19)2018.PubMed/NCBI View Article : Google Scholar

12 

Wang J, Zhu H, Huang L, Zhu X, Sha J, Li G, Ma G, Zhang W, Gu M and Guo Y: Nrf2 signaling attenuates epithelial-to-mesenchymal transition and renal interstitial fibrosis via PI3K/Akt signaling pathways. Exp Mol Pathol. 111(104296)2019.PubMed/NCBI View Article : Google Scholar

13 

Wakui M, Fujimori Y, Nakamura S, Kondo Y, Kuroda Y, Oka S, Nakagawa T, Katagiri H and Murata M: Distinct features of bivalent direct thrombin inhibitors, hirudin and bivalirudin, revealed by clot waveform analysis and enzyme kinetics in coagulation assays. J Clin Pathol. 72:817–824. 2019.PubMed/NCBI View Article : Google Scholar

14 

Deng F, Zhang J, Li Y, Wang W, Hong D, Li G and Feng J: Hirudin ameliorates immunoglobulin A nephropathy by inhibition of fibrosis and inflammatory response. Ren Fail. 41:104–112. 2019.PubMed/NCBI View Article : Google Scholar

15 

Xie Y, Lan F, Zhao J and Shi W: Hirudin improves renal interstitial fibrosis by reducing renal tubule injury and inflammation in unilateral ureteral obstruction (UUO) mice. Int Immunopharmacol. 81(106249)2020.PubMed/NCBI View Article : Google Scholar

16 

Bae JS, Kim IS and Rezaie AR: Thrombin down-regulates the TGF-beta-mediated synthesis of collagen and fibronectin by human proximal tubule epithelial cells through the EPCR-dependent activation of PAR-1. J Cell Physiol. 225:233–239. 2010.PubMed/NCBI View Article : Google Scholar

17 

Sun XJ, Chen M and Zhao MH: Thrombin contributes to anti-myeloperoxidase antibody positive IgG-mediated glomerular endothelial cells activation through SphK1-S1P-S1PR3 signaling. Front Immunol. 10(237)2019.PubMed/NCBI View Article : Google Scholar

18 

Mahajan-Thakur S, Sostmann BD, Fender AC, Behrendt D, Felix SB, Schrör K and Rauch BH: Sphingosine-1-phosphate induces thrombin receptor PAR-4 expression to enhance cell migration and COX-2 formation in human monocytes. J Leukoc Biol. 96:611–618. 2014.PubMed/NCBI View Article : Google Scholar

19 

Wang GL, Xia XL, Li XL, He FH and Li JL: Identification and expression analysis of the MSP130-related-2 gene from Hyriopsis cumingii. Genet Mol Res. 14:4903–4913. 2015.PubMed/NCBI View Article : Google Scholar

20 

Hirata N, Yamada S, Shoda T, Kurihara M, Sekino Y and Kanda Y: Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation. Nat Commun. 5(4806)2014.PubMed/NCBI View Article : Google Scholar

21 

Hsieh PF, Liu SF, Lee TC, Huang JS, Yin LT, Chang WT, Chuang LY, Guh JY, Hung MY and Yang YL: The role of IL-7 in renal proximal tubule epithelial cells fibrosis. Mol Immunol. 50:74–82. 2012.PubMed/NCBI View Article : Google Scholar

22 

Chen HA, Chen CM, Guan SS, Chiang CK, Wu CT and Liu SH: The antifibrotic and anti-inflammatory effects of icariin on the kidney in a unilateral ureteral obstruction mouse model. Phytomedicine. 59(152917)2019.PubMed/NCBI View Article : Google Scholar

23 

Chen DQ, Cao G, Zhao H, Chen L, Yang T, Wang M, Vaziri ND, Guo Y and Zhao YY: Combined melatonin and poricoic acid A inhibits renal fibrosis through modulating the interaction of Smad3 and β-catenin pathway in AKI-to-CKD continuum. Ther Adv Chronic Dis. 10(2040622319869116)2019.PubMed/NCBI View Article : Google Scholar

24 

Chen DQ, Feng YL, Cao G and Zhao YY: Natural products as a source for antifibrosis therapy. Trends Pharmacol Sci. 39:937–952. 2018.PubMed/NCBI View Article : Google Scholar

25 

Liu X, Hong Q, Wang Z, Yu Y, Zou X and Xu L: Transforming growth factor-β-sphingosine kinase 1/S1P signaling upregulates microRNA-21 to promote fibrosis in renal tubular epithelial cells. Exp Biol Med (Maywood). 241:265–272. 2016.PubMed/NCBI View Article : Google Scholar

26 

Yang Z, Xiong F, Wang Y, Gong W, Huang J, Chen C, Liu P and Huang H: TGR5 activation suppressed S1P/S1P2 signaling and resisted high glucose-induced fibrosis in glomerular mesangial cells. Pharmacol Res. 111:226–236. 2016.PubMed/NCBI View Article : Google Scholar

27 

Man XY, Chen XB, Li W, Landeck L, Dou TT, Chen JQ, Zhou J, Cai SQ and Zheng M: Analysis of epithelial-mesenchymal transition markers in psoriatic epidermal keratinocytes. Open Biol. 5(150032)2015.PubMed/NCBI View Article : Google Scholar

28 

Xu R, Chen MY, Liang W, Chen Y and Guo MY: Zinc deficiency aggravation of ROS and inflammatory injury leading to renal fibrosis in mice. Biol Trace Elem Res. 199:622–632. 2021.PubMed/NCBI View Article : Google Scholar

29 

Posma JJ, Grover SP, Hisada Y, Owens AP III, Antoniak S, Spronk HM and Mackman N: Roles of coagulation proteases and PARs (protease-activated receptors) in mouse models of inflammatory diseases. Arterioscler Thromb Vasc Biol. 39:13–24. 2019.PubMed/NCBI View Article : Google Scholar

30 

Liang D, Song Z, Liang W, Li Y and Liu S: Metformin inhibits TGF-beta 1-induced MCP-1 expression through BAMBI-mediated suppression of MEK/ERK1/2 signalling. Nephrology (Carlton). 24:481–488. 2019.PubMed/NCBI View Article : Google Scholar

31 

Waasdorp M, de Rooij DM, Florquin S, Duitman J and Spek CA: Protease-activated receptor-1 contributes to renal injury and interstitial fibrosis during chronic obstructive nephropathy. J Cell Mol Med. 23:1268–1279. 2019.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Lin Q, Long C, Wang Z, Wang R, Shi W, Qiu J, Mo J and Xie Y: Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling. Exp Ther Med 23: 3, 2022.
APA
Lin, Q., Long, C., Wang, Z., Wang, R., Shi, W., Qiu, J. ... Xie, Y. (2022). Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling. Experimental and Therapeutic Medicine, 23, 3. https://doi.org/10.3892/etm.2021.10924
MLA
Lin, Q., Long, C., Wang, Z., Wang, R., Shi, W., Qiu, J., Mo, J., Xie, Y."Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling". Experimental and Therapeutic Medicine 23.1 (2022): 3.
Chicago
Lin, Q., Long, C., Wang, Z., Wang, R., Shi, W., Qiu, J., Mo, J., Xie, Y."Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling". Experimental and Therapeutic Medicine 23, no. 1 (2022): 3. https://doi.org/10.3892/etm.2021.10924
Copy and paste a formatted citation
x
Spandidos Publications style
Lin Q, Long C, Wang Z, Wang R, Shi W, Qiu J, Mo J and Xie Y: Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling. Exp Ther Med 23: 3, 2022.
APA
Lin, Q., Long, C., Wang, Z., Wang, R., Shi, W., Qiu, J. ... Xie, Y. (2022). Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling. Experimental and Therapeutic Medicine, 23, 3. https://doi.org/10.3892/etm.2021.10924
MLA
Lin, Q., Long, C., Wang, Z., Wang, R., Shi, W., Qiu, J., Mo, J., Xie, Y."Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling". Experimental and Therapeutic Medicine 23.1 (2022): 3.
Chicago
Lin, Q., Long, C., Wang, Z., Wang, R., Shi, W., Qiu, J., Mo, J., Xie, Y."Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling". Experimental and Therapeutic Medicine 23, no. 1 (2022): 3. https://doi.org/10.3892/etm.2021.10924
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