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MicroRNA‑367‑3p suppresses sevoflurane‑induced adult rat astrocyte apoptosis by targeting BCL2L11

  • Authors:
    • Deming Xu
    • Changbi Zhou
    • Juanyun Lin
    • Wenhui Cai
    • Wei Lin
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The Affiliated Hospital of Putian University, Putian, Fujian 351100, P.R. China, Department of General Surgery, The Affiliated Hospital of Putian University, Putian, Fujian 351100, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 9
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    Published online on: October 28, 2021
       https://doi.org/10.3892/etm.2021.10931
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Abstract

The present study aimed to characterize the effect of microRNA (miR)‑367‑3p on sevoflurane anesthesia and elucidate the underlying mechanism. A total of 36 4‑month‑old adult Sprague‑Dawley rats were divided into six groups. Sevoflurane was inhaled at concentrations of 0, 1, 2, 4, 8 and 16% for a total of 6 h; the hippocampus of the brain was subsequently minced and digested, and astrocytes were isolated. Various methods, including reverse transcription‑quantitative (RT‑q)PCR, western blotting and TUNEL staining, were used to determine the expression levels of Bax, BCL‑2 and BCL‑2‑like protein 11 (BCL2L11), as well as the level of apoptosis. The rats were treated with 8% sevoflurane and the astrocytes from the rats were transfected with miR‑367‑3p or anti‑miR‑367‑3p. The present study demonstrated that sevoflurane promoted astrocytes apoptosis. Western blotting revealed that with an increase of sevoflurane concentration, the expression levels of the apoptotic proteins Bax and BCL2L11 were significantly increased, whereas the protein expression levels of BCL‑2 were significantly decreased. However, overexpression of miR‑367‑3p reversed these effects. TUNEL staining revealed that sevoflurane promoted the apoptosis of astrocytes, while apoptosis was reversed by miR‑367‑3p overexpression. RT‑qPCR demonstrated that sevoflurane inhibited the expression of miR‑367‑3p. Notably, miR‑367‑3p reduced the expression of BCL2L11, thereby inhibiting the apoptosis of astrocytes originating from the hippocampal area of adult rats induced by sevoflurane. Therefore, miR‑367‑3p and BCL2L11 may act as effective targets for the study of anesthesia.
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Copy and paste a formatted citation
Spandidos Publications style
Xu D, Zhou C, Lin J, Cai W and Lin W: MicroRNA‑367‑3p suppresses sevoflurane‑induced adult rat astrocyte apoptosis by targeting BCL2L11. Exp Ther Med 23: 9, 2022.
APA
Xu, D., Zhou, C., Lin, J., Cai, W., & Lin, W. (2022). MicroRNA‑367‑3p suppresses sevoflurane‑induced adult rat astrocyte apoptosis by targeting BCL2L11. Experimental and Therapeutic Medicine, 23, 9. https://doi.org/10.3892/etm.2021.10931
MLA
Xu, D., Zhou, C., Lin, J., Cai, W., Lin, W."MicroRNA‑367‑3p suppresses sevoflurane‑induced adult rat astrocyte apoptosis by targeting BCL2L11". Experimental and Therapeutic Medicine 23.1 (2022): 9.
Chicago
Xu, D., Zhou, C., Lin, J., Cai, W., Lin, W."MicroRNA‑367‑3p suppresses sevoflurane‑induced adult rat astrocyte apoptosis by targeting BCL2L11". Experimental and Therapeutic Medicine 23, no. 1 (2022): 9. https://doi.org/10.3892/etm.2021.10931
Copy and paste a formatted citation
x
Spandidos Publications style
Xu D, Zhou C, Lin J, Cai W and Lin W: MicroRNA‑367‑3p suppresses sevoflurane‑induced adult rat astrocyte apoptosis by targeting BCL2L11. Exp Ther Med 23: 9, 2022.
APA
Xu, D., Zhou, C., Lin, J., Cai, W., & Lin, W. (2022). MicroRNA‑367‑3p suppresses sevoflurane‑induced adult rat astrocyte apoptosis by targeting BCL2L11. Experimental and Therapeutic Medicine, 23, 9. https://doi.org/10.3892/etm.2021.10931
MLA
Xu, D., Zhou, C., Lin, J., Cai, W., Lin, W."MicroRNA‑367‑3p suppresses sevoflurane‑induced adult rat astrocyte apoptosis by targeting BCL2L11". Experimental and Therapeutic Medicine 23.1 (2022): 9.
Chicago
Xu, D., Zhou, C., Lin, J., Cai, W., Lin, W."MicroRNA‑367‑3p suppresses sevoflurane‑induced adult rat astrocyte apoptosis by targeting BCL2L11". Experimental and Therapeutic Medicine 23, no. 1 (2022): 9. https://doi.org/10.3892/etm.2021.10931
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