MicroRNA‑499a (rs3746444A/G) gene variant and susceptibility to type 2 diabetes‑associated end‑stage renal disease

Fawzy,Manal S.; Al Ageeli,Essam; Al‑Qahtani,Saeed Awad; Abu Alsel,Baraah T.; Kattan,Shahad W.; Alelwani,Walla; Toraih,Eman A.

doi:10.3892/etm.2021.10985

MicroRNA‑499a (rs3746444A/G) gene variant and susceptibility to type 2 diabetes‑associated end‑stage renal disease

Authors:
- Manal S. Fawzy
- Essam Al Ageeli
- Saeed Awad Al‑Qahtani
- Baraah T. Abu Alsel
- Shahad W. Kattan
- Walla Alelwani
- Eman A. Toraih
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Affiliations: Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt, Department of Clinical Biochemistry (Medical Genetics), Faculty of Medicine, Jazan University, Jazan 45142, Saudi Arabia, Department of Physiology, Faculty of Medicine, Taibah University, Al Madinah Al Munawwarah 42353, Saudi Arabia, Department of Pathology, Northern Border University, Arar 1321, Saudi Arabia, Department of Medical Laboratory, College of Applied Medical Sciences, Taibah University, Yanbu 46522, Saudi Arabia, Department of Biochemistry, Faculty of Science, University of Jeddah, Jeddah 23445, Saudi Arabia, Department of Surgery, School of Medicine, Tulane University, New Orleans, LA 70112‑2632, USA
Published online on: November 22, 2021 https://doi.org/10.3892/etm.2021.10985
Article Number: 63
Copyright: © Fawzy et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Diabetic nephropathy (DN) is a major risk factor for end‑stage renal disease (ESRD). MicroRNAs (miRNAs/miRs) and their variants may be implicated in health and disease, including DN. The present study aimed to investigate the association of the miRNA‑499a gene (MIR499A) A/G seed region variant (rs3746444) with DN‑associated ESRD susceptibility in patients with diabetes mellitus, and to determine whether there was an association between the different genotypes and the patients' laboratory and clinical data. A case‑control pilot study was conducted on 180 adult patients with type 2 diabetes mellitus. A total of 90 patients with ESRD on regular hemodialysis were considered as the cases, and 90 age‑, sex‑ and ethnicity‑matched diabetic patients with normo‑albuminuria were considered as the controls. MIR499A genotyping was performed using a TaqMan Real‑Time allele discrimination assay. Results demonstrated that the MIR499A rs3746444*G variant conferred susceptibility to the development of ESRD under co‑dominant [(odds ratio (95% confidence interval): 2.49 (1.41‑3.89) and 2.41 (1.61‑6.68) for heterozygous and homozygous comparison, respectively], dominant [2.30 (1.18‑3.90)] and allelic [1.82 (1.17‑2.83)] models. Different genotypes of the specified variant did not exhibit significant associations with the clinic‑laboratory data of the studied patients or the circulating miR‑499a plasma levels. In conclusion, results of the present study suggested that MIR499A rs3746444 may be a susceptibility variant for DN‑associated ESRD in the study population. However, larger sample size studies with different ethnicities are warranted to verify these findings.

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