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Paeonol suppresses lipid formation and promotes lipid degradation in adipocytes

  • Authors:
    • Ji Li
    • Huan Gu
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Guang'anmen Hospital, Chinese Academy of Traditional Chinese Medicine, Beijing 100053, P.R. China, Department of Cardiology of Integrated Traditional Chinese and Western Medicine, China‑Japan Friendship Hospital, Beijing 100029, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 78
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    Published online on: November 25, 2021
       https://doi.org/10.3892/etm.2021.11001
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Abstract

Paeonol can regulate a variety of physiological and pathological processes such as thrombosis, oxidative stress, inflammation and atherosclerosis. However, its potential role and underlying mechanisms in obesity and lipid metabolism remain to be elucidated. In the present study, 3T3‑L1 cells were differentiated and collected on days 4, 6 and 8. The expression levels of fatty‑acid‑binding protein 4 (FABP4) and microRNA (miR)‑21 were detected using reverse transcription‑quantitative PCR and western blot analyses. Cell viability was assessed using a Cell Counting Kit‑8 assay. A miR‑21 mimic was constructed and transfected into 3T3‑L1 preadipocytes. Adipocyte differentiation was detected using Oil Red O staining. The proteins CD36, glucose transporter 4, peroxisome proliferator‑activated receptor γ (PPAR‑γ) and adipocyte protein 2 (Ap2) were detected using western blot analysis. The expression levels of FABP4 and miR‑21 were increased in differentiated 3T3‑L1 cells. Paeonol exhibited no effects on cell activity, whereas it inhibited the expression levels of miR‑21 in the 3T3‑L1 differentiated adipocytes. Paeonol suppressed the differentiation of 3T3‑L1 adipocytes and its effect was partially reversed by the overexpression of miR‑21. In addition, paeonol promoted the lipid degradation of 3T3‑L1 adipocytes, increased the expression levels of PPAR‑γ and Ap2, and suppressed triglyceride synthesis in these cells. These effects were partially reversed by the overexpression of miR‑21. In conclusion, the findings of the present study indicated that paeonol may exert protective effects against lipid formation and promote lipid degradation in adipocytes. These data provide evidence of the regulatory effect of paeonol on adipocyte differentiation and highlight its pathological significance.
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1 

Masukume G, O'Neill SM, Baker PN, Kenny LC, Morton SMB and Khashan AS: The impact of Caesarean section on the risk of childhood overweight and obesity: new evidence from a contemporary cohort study. Sci Rep. 8(15113)2018.PubMed/NCBI View Article : Google Scholar

2 

Wang L, Wang H, Zhang B, Popkin BM and Du S: Elevated fat intake increases body weight and the risk of overweight and obesity among Chinese adults: 1991-2015 trends. Nutrients. 12(3272)2020.PubMed/NCBI View Article : Google Scholar

3 

Akici N, Onal ZE, Gürbüz T, Sağ C and Kilinç S: Atherogenic indices in the assessment of cardiovascular disease risk in children with obesity and subclinical hypothyroidism. Acta Endocrinol (Bucur). 16:334–338. 2020.PubMed/NCBI View Article : Google Scholar

4 

Izumi M, Yoshida T, Nakamura T and Wakamori AM: Paeonol, an Ingredient of Kamishoyosan, Reduces Intracellular Lipid Accumulation by Inhibiting Glucocorticoid Receptor Activity in 3T3-L1 Cells. Nutrients. 12(12)2020.PubMed/NCBI View Article : Google Scholar

5 

Al-Taher AY, Morsy MA, Rifaai RA, Zenhom NM and Abdel-Gaber SA: Paeonol attenuates methotrexate-induced cardiac toxicity in rats by inhibiting oxidative stress and suppressing TLR4-induced NF-κB inflammatory pathway. Mediators Inflamm. 2020(8641026)2020.PubMed/NCBI View Article : Google Scholar

6 

Ramachandhiran D, Vinothkumar V and Babukumar S: Paeonol exhibits anti-tumor effects by apoptotic and anti-inflammatory activities in 7,12-dimethylbenz(a)anthracene induced oral carcinogenesis. Biotech Histochem. 94:10–25. 2019.PubMed/NCBI View Article : Google Scholar

7 

Adki KM and Kulkarni YA: Neuroprotective effect of paeonol in streptozotocin-induced diabetes in rats. Life Sci. 271(119202)2021.PubMed/NCBI View Article : Google Scholar

8 

Xu F, Xiao H, Liu R, Yang Y, Zhang M, Chen L, Chen Z, Liu P and Huang H: Paeonol ameliorates glucose and lipid metabolism in experimental diabetes by activating Akt. Front Pharmacol. 10(261)2019.PubMed/NCBI View Article : Google Scholar

9 

Ping M, Xiao W, Mo L, Xiao X, Song S, Tang W and Yang X: Paeonol attenuates advanced oxidation protein product-induced oxidative stress injury in THP-1 macrophages. Pharmacology. 93:286–295. 2014.PubMed/NCBI View Article : Google Scholar

10 

Li H, Dai M and Jia W: Paeonol attenuates high-fat-diet-induced atherosclerosis in rabbits by anti-inflammatory activity. Planta Med. 75:7–11. 2009.PubMed/NCBI View Article : Google Scholar

11 

Yu Y, Yan R, Chen X, Sun T and Yan J: Paeonol suppresses the effect of ox-LDL on mice vascular endothelial cells by regulating miR-338-3p/TET2 axis in atherosclerosis. Mol Cell Biochem. 475:127–135. 2020.PubMed/NCBI View Article : Google Scholar

12 

Zhong LJ, Xie ZS, Yang H, Li P and Xu XJ: Moutan Cortex and Paeoniae Radix Rubra reverse high-fat-diet-induced metabolic disorder and restore gut microbiota homeostasis. Chin J Nat Med. 15:210–219. 2017.PubMed/NCBI View Article : Google Scholar

13 

Melkman-Zehavi T, Oren R, Kredo-Russo S, Shapira T, Mandelbaum AD, Rivkin N, Nir T, Lennox KA, Behlke MA, Dor Y, et al: miRNAs control insulin content in pancreatic β-cells via downregulation of transcriptional repressors. EMBO J. 30:835–845. 2011.PubMed/NCBI View Article : Google Scholar

14 

Ma E, Fu Y and Garvey WT: Relationship of circulating miRNAs with insulin sensitivity and associated metabolic risk factors in humans. Metab Syndr Relat Disord. 16:82–89. 2018.PubMed/NCBI View Article : Google Scholar

15 

Lang H, Xiang Y, Lin N, Ai Z, You Z, Xiao J, Liu D and Yang Y: Identification of a panel of miRNAs as positive regulators of insulin release in pancreatic β-cells. Cell Physiol Biochem. 48:185–193. 2018.PubMed/NCBI View Article : Google Scholar

16 

Meng S, Cao JT, Zhang B, Zhou Q, Shen CX and Wang CQ: Downregulation of microRNA-126 in endothelial progenitor cells from diabetes patients, impairs their functional properties, via target gene Spred-1. J Mol Cell Cardiol. 53:64–72. 2012.PubMed/NCBI View Article : Google Scholar

17 

Tomé-Carneiro J, Larrosa M, Yáñez-Gascón MJ, Dávalos A, Gil-Zamorano J, Gonzálvez M, García-Almagro FJ, Ruiz Ros JA, Tomás-Barberán FA, Espín JC, et al: One-year supplementation with a grape extract containing resveratrol modulates inflammatory-related microRNAs and cytokines expression in peripheral blood mononuclear cells of type 2 diabetes and hypertensive patients with coronary artery disease. Pharmacol Res. 72:69–82. 2013.PubMed/NCBI View Article : Google Scholar

18 

Liu YR, Chen JJ and Dai M: Paeonol protects rat vascular endothelial cells from ox-LDL-induced injury in vitro via downregulating microRNA-21 expression and TNF-α release. Acta Pharmacol Sin. 35:483–488. 2014.PubMed/NCBI View Article : Google Scholar

19 

Kang M, Yan LM, Zhang WY, Li YM, Tang AZ and Ou HS: Role of microRNA-21 in regulating 3T3-L1 adipocyte differentiation and adiponectin expression. Mol Biol Rep. 40:5027–5034. 2013.PubMed/NCBI View Article : Google Scholar

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

21 

Mitterberger MC, Lechner S, Mattesich M, Kaiser A, Probst D, Wenger N, Pierer G and Zwerschke W: DLK1(PREF1) is a negative regulator of adipogenesis in CD105+/CD90+/CD34+/CD31-/FABP4- adipose-derived stromal cells from subcutaneous abdominal fat pats of adult women. Stem Cell Res. 9:35–48. 2012.PubMed/NCBI View Article : Google Scholar

22 

Lee SC, Lee YJ, Shin MK and Sung JS: Regulation of CXCR6 expression on adipocytes and osteoblasts differentiated from human adipose tissue-derived mesenchymal stem cells. Stem Cells Int. 2020(8870133)2020.PubMed/NCBI View Article : Google Scholar

23 

Jiang R, Yang T, Zhang Y, Wang Z and Zhang T: LKB1 promotes the transformation of bone marrow mesenchymal stem cells into adipocytes under oxidative stress via AMPK-mTOR signaling pathway. J Interferon Cytokine Res. 40:370–376. 2020.PubMed/NCBI View Article : Google Scholar

24 

Zhang J, Cai B, Ma M, Luo W, Zhang Z, Zhang X and Nie Q: ALDH1A1 inhibits chicken preadipocytes' proliferation and differentiation via the PPARγ pathway in vitro and in vivo. Int J Mol Sci. 21(3150)2020.PubMed/NCBI View Article : Google Scholar

25 

Pacifici F, Farias CLA, Rea S, Capuani B, Feraco A, Coppola A, Mammi C, Pastore D, Abete P, Rovella V, et al: Tyrosol may prevent obesity by inhibiting adipogenesis in 3T3-L1 preadipocytes. Oxid Med Cell Longev. 2020(4794780)2020.PubMed/NCBI View Article : Google Scholar

26 

Son Y, Cox JM, Stevenson JL, Cooper JA and Paton CM: Angiopoietin-1 protects 3T3-L1 preadipocytes from saturated fatty acid-induced cell death. Nutr Res. 76:20–28. 2020.PubMed/NCBI View Article : Google Scholar

27 

Kurt S, Gürkan CG, Keleş Tezal GC, Çiftçi A, Gürgör PN, Güler Ş and Çetinkaya BÖ: Histopathological and biochemical evaluation of the effect of Paeoniflorin on the periodontium during and after periodontitis formation in rats. Arch Oral Biol. 102:135–140. 2019.PubMed/NCBI View Article : Google Scholar

28 

Ni J, Yang D, Song L and Li C: Protective effects of paeoniflorin on alveolar bone resorption and soft-tissue breakdown in experimental periodontitis. J Periodontal Res. 51:257–264. 2016.PubMed/NCBI View Article : Google Scholar

29 

Qiu J, Chen M, Liu J, Huang X, Chen J, Zhou L, Ma J, Sextius P, Pena AM, Cai Z, et al: The skin-depigmenting potential of Paeonia lactiflora root extract and paeoniflorin: In vitro evaluation using reconstructed pigmented human epidermis. Int J Cosmet Sci. 38:444–451. 2016.PubMed/NCBI View Article : Google Scholar

30 

Baek K and Baek JH: The transcription factors myeloid elf-1-like factor (MEF) and distal-less homeobox 5 (Dlx5) inversely regulate the differentiation of osteoblasts and adipocytes in bone marrow. Adipocyte. 2:50–54. 2013.PubMed/NCBI View Article : Google Scholar

31 

Wang P, Ji R, Ji J and Chen F: Changes of metabolites of acrylamide and glycidamide in acrylamide-exposed rats pretreated with blueberry anthocyanins extract. Food Chem. 274:611–619. 2019.PubMed/NCBI View Article : Google Scholar

32 

Kim JH, Lee S, Kim HY and Cho EJ: Acer okamotoanum inhibits adipocyte differentiation by the regulation of adipogenesis and lipolysis in 3T3-L1 cells. Int J Mol Med. 45:589–596. 2020.PubMed/NCBI View Article : Google Scholar

33 

Curcio CA, Johnson M, Rudolf M and Huang JD: The oil spill in ageing Bruch membrane. Br J Ophthalmol. 95:1638–1645. 2011.PubMed/NCBI View Article : Google Scholar

34 

Liu HY, Zhang YY, Zhu BL, Feng FZ, Yan H, Zhang HY and Zhou B: miR-21 regulates the proliferation and apoptosis of ovarian cancer cells through PTEN/PI3K/AKT. Eur Rev Med Pharmacol Sci. 23:4149–4155. 2019.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Li J and Gu H: Paeonol suppresses lipid formation and promotes lipid degradation in adipocytes. Exp Ther Med 23: 78, 2022.
APA
Li, J., & Gu, H. (2022). Paeonol suppresses lipid formation and promotes lipid degradation in adipocytes. Experimental and Therapeutic Medicine, 23, 78. https://doi.org/10.3892/etm.2021.11001
MLA
Li, J., Gu, H."Paeonol suppresses lipid formation and promotes lipid degradation in adipocytes". Experimental and Therapeutic Medicine 23.1 (2022): 78.
Chicago
Li, J., Gu, H."Paeonol suppresses lipid formation and promotes lipid degradation in adipocytes". Experimental and Therapeutic Medicine 23, no. 1 (2022): 78. https://doi.org/10.3892/etm.2021.11001
Copy and paste a formatted citation
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Spandidos Publications style
Li J and Gu H: Paeonol suppresses lipid formation and promotes lipid degradation in adipocytes. Exp Ther Med 23: 78, 2022.
APA
Li, J., & Gu, H. (2022). Paeonol suppresses lipid formation and promotes lipid degradation in adipocytes. Experimental and Therapeutic Medicine, 23, 78. https://doi.org/10.3892/etm.2021.11001
MLA
Li, J., Gu, H."Paeonol suppresses lipid formation and promotes lipid degradation in adipocytes". Experimental and Therapeutic Medicine 23.1 (2022): 78.
Chicago
Li, J., Gu, H."Paeonol suppresses lipid formation and promotes lipid degradation in adipocytes". Experimental and Therapeutic Medicine 23, no. 1 (2022): 78. https://doi.org/10.3892/etm.2021.11001
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