Open Access

Transplantation of IL‑1β siRNA‑modified bone marrow mesenchymal stem cells ameliorates type II collagen‑induced rheumatoid arthritis in rats

  • Authors:
    • Shifeng Pan
    • Xuan Dong
    • Yan Wang
    • Tiansheng Zhou
    • Yuting Liu
    • An Zhou
    • Hua Xing
  • View Affiliations

  • Published online on: December 14, 2021     https://doi.org/10.3892/etm.2021.11062
  • Article Number: 139
  • Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Rheumatoid arthritis (RA) is a chronic autoimmune disease that causes erosion of articular cartilage and bone and has adverse effects on both patients and livestock animals. The aim of the present study was to investigate the role of interleukin‑1β (IL‑1β) in the pathogenesis of RA, and to further determine whether injection of IL‑1β small interfering RNA (siRNA) or transplantation of IL‑1β siRNA + bone marrow mesenchymal stem cells (BMSCs) can ameliorate RA in rats. A collagen‑induced arthritis (CIA) rat model was established by injecting type II collagen for 4 weeks. Next, CIA rats were randomly divided into three groups and injected or transplanted with PBS, IL‑1β siRNA and IL‑1β siRNA + BMSCs for another 4 weeks. The CIA rat model was successfully established, as demonstrated by the higher toe swelling value, thymus and spleen/body weight, immobility time and serum IL‑1β concentration, as well as lower body weight, climbing time and mRNA expression of programmed death‑1 (PD‑1), transforming growth factor‑β1 (TGF‑β1) and forkhead box protein 3 (Foxp3) in the spleen, compared with control rats. Furthermore, histopathology results demonstrated that joint swelling and redness were observed in the knee joints of CIA rats. H&E results revealed that CIA rats presented erosive destruction of the bone and ulceration of the articular cartilage. In addition, in vitro results demonstrated that IL‑1β expression was successfully silenced after IL‑1β siRNA transfection in lipopolysaccharide‑stimulated BMSCs. When compared with the results of PBS rats, both IL‑1β siRNA injection and IL‑1β siRNA + BMSC transplantation significantly increased the body weight, climbing time and mRNA expression of PD‑1, TGF‑β1 and Foxp3 in the spleen, while significantly reduced the immobility time and serum IL‑1β concentration. In addition, when compared with that of IL‑1β siRNA injection, IL‑1β siRNA + BMSC transplantation exhibited markedly higher therapeutic efficacy against CIA. These results demonstrated that higher IL‑1β contributed to the pathogenesis of CIA, and that IL‑1β siRNA injection ameliorated CIA, while its combination with BMSCs exerted synergistic effects, which may be beneficial against RA.
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February-2022
Volume 23 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Pan S, Dong X, Wang Y, Zhou T, Liu Y, Zhou A and Xing H: Transplantation of IL‑1β siRNA‑modified bone marrow mesenchymal stem cells ameliorates type II collagen‑induced rheumatoid arthritis in rats. Exp Ther Med 23: 139, 2022
APA
Pan, S., Dong, X., Wang, Y., Zhou, T., Liu, Y., Zhou, A., & Xing, H. (2022). Transplantation of IL‑1β siRNA‑modified bone marrow mesenchymal stem cells ameliorates type II collagen‑induced rheumatoid arthritis in rats. Experimental and Therapeutic Medicine, 23, 139. https://doi.org/10.3892/etm.2021.11062
MLA
Pan, S., Dong, X., Wang, Y., Zhou, T., Liu, Y., Zhou, A., Xing, H."Transplantation of IL‑1β siRNA‑modified bone marrow mesenchymal stem cells ameliorates type II collagen‑induced rheumatoid arthritis in rats". Experimental and Therapeutic Medicine 23.2 (2022): 139.
Chicago
Pan, S., Dong, X., Wang, Y., Zhou, T., Liu, Y., Zhou, A., Xing, H."Transplantation of IL‑1β siRNA‑modified bone marrow mesenchymal stem cells ameliorates type II collagen‑induced rheumatoid arthritis in rats". Experimental and Therapeutic Medicine 23, no. 2 (2022): 139. https://doi.org/10.3892/etm.2021.11062