miR‑340‑5p inhibits pancreatic acinar cell inflammation and apoptosis via targeted inhibition of HMGB1

  • Authors:
    • Yazhou Gao
    • Liming Wang
    • Zequn Niu
    • Hui Feng
    • Jie Liu
    • Jiangli Sun
    • Yanxia Gao
    • Longfei Pan
  • View Affiliations

  • Published online on: December 14, 2021     https://doi.org/10.3892/etm.2021.11063
  • Article Number: 140
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Abstract

Acute pancreatitis (AP) is a common gastrointestinal disease that affects 1 million individuals worldwide. Inflammation and apoptosis are considered to be important pathogenic mechanisms of AP, and high mobility group box 1 (HMGB1) has been shown to play a particularly important role in the etiology of this disease. MicroRNAs (miRs) are emerging as critical regulators of gene expression and, as such, they represent a promising area of therapeutic target identification and development for a variety of diseases, including AP. Using the online database query (microRNA.org), the current study identified a site in the 3' untranslated region of HMGB1 mRNA that was a viable target for miR‑340‑5p. The present study aimed to investigate the association between miR‑340‑5p and HMGB1 expression in pancreatic acinar cells following lipopolysaccharide (LPS) treatment by performing luciferase, western blotting and reverse transcription‑quantitative PCR assays. The results suggest that miR‑340‑5p attenuates the induction of HMGB1 by LPS, thereby inhibiting inflammation and apoptosis via blunted activation of Toll‑like receptor 4 and enhanced AKT signaling. Thus, the therapeutic application of miR‑340‑5p may be a useful strategy in AP via upregulation of HMGB1 and subsequent promotion of inflammation and apoptosis.
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February-2022
Volume 23 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Gao Y, Wang L, Niu Z, Feng H, Liu J, Sun J, Gao Y and Pan L: miR‑340‑5p inhibits pancreatic acinar cell inflammation and apoptosis via targeted inhibition of HMGB1. Exp Ther Med 23: 140, 2022
APA
Gao, Y., Wang, L., Niu, Z., Feng, H., Liu, J., Sun, J. ... Pan, L. (2022). miR‑340‑5p inhibits pancreatic acinar cell inflammation and apoptosis via targeted inhibition of HMGB1. Experimental and Therapeutic Medicine, 23, 140. https://doi.org/10.3892/etm.2021.11063
MLA
Gao, Y., Wang, L., Niu, Z., Feng, H., Liu, J., Sun, J., Gao, Y., Pan, L."miR‑340‑5p inhibits pancreatic acinar cell inflammation and apoptosis via targeted inhibition of HMGB1". Experimental and Therapeutic Medicine 23.2 (2022): 140.
Chicago
Gao, Y., Wang, L., Niu, Z., Feng, H., Liu, J., Sun, J., Gao, Y., Pan, L."miR‑340‑5p inhibits pancreatic acinar cell inflammation and apoptosis via targeted inhibition of HMGB1". Experimental and Therapeutic Medicine 23, no. 2 (2022): 140. https://doi.org/10.3892/etm.2021.11063