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Therapeutic effect and mechanism of 4‑phenyl butyric acid on renal ischemia‑reperfusion injury in mice

  • Authors:
    • Xinlei Wang
    • Yang Zhang
    • Kun Wuyun
    • Haixia Gong
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 144
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    Published online on: December 15, 2021
       https://doi.org/10.3892/etm.2021.11067
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Abstract

The aim of the present study was to explore the effects and possible mechanism of 4‑phenylbutyric acid (4‑PBA) on renal ischemia‑reperfusion injury (RIRI) in mice. A RIRI model of HK‑2 cells was constructed using hypoxia/reoxygenation (H/R) treatment. Dexmedetomidine and 4‑PBA were used to treat the cells before and after modeling. Apoptosis and expression levels of cyclophilin D (CypD), cytochrome c, eukaryotic translation initiation factor 2α (eIF2α), glucose‑regulated protein 78 (GRP78), intercellular adhesion molecule (ICAM)‑1 and vascular adhesion molecule (VCAM)‑1 were measured using flow cytometry, western blotting and immunohistochemistry. The renal volume, weight and renal arterial resistance index (RRI) were determined using the renal ischemia model. Compared with untreated model cells, 4‑PBA treatment significantly decreased apoptosis and the expression levels of CypD, Cytochrome c, eIF2α and GRP78 in HK‑2 cells. There was no significant change in renal volume and weight after modeling, but RRI was significantly decreased after 4‑PBA treatments in the model. Western blotting and immunohistochemistry analysis demonstrated that 4‑PBA treatment also significantly decreased the expression of ICAM‑1 and VCAM‑1. Overall, 4‑PBA had a therapeutic effect on RIRI in mice. This protection may be mediated by decreasing the expression levels of CypD, Cytochrome c, eIF2α and GRP78, and subsequent reduction of cellular oxygen free radicals and apoptosis, leading to an alleviated endoplasmic reticulum stress response and RIRI.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Zhang Y, Wuyun K and Gong H: Therapeutic effect and mechanism of 4‑phenyl butyric acid on renal ischemia‑reperfusion injury in mice. Exp Ther Med 23: 144, 2022.
APA
Wang, X., Zhang, Y., Wuyun, K., & Gong, H. (2022). Therapeutic effect and mechanism of 4‑phenyl butyric acid on renal ischemia‑reperfusion injury in mice. Experimental and Therapeutic Medicine, 23, 144. https://doi.org/10.3892/etm.2021.11067
MLA
Wang, X., Zhang, Y., Wuyun, K., Gong, H."Therapeutic effect and mechanism of 4‑phenyl butyric acid on renal ischemia‑reperfusion injury in mice". Experimental and Therapeutic Medicine 23.2 (2022): 144.
Chicago
Wang, X., Zhang, Y., Wuyun, K., Gong, H."Therapeutic effect and mechanism of 4‑phenyl butyric acid on renal ischemia‑reperfusion injury in mice". Experimental and Therapeutic Medicine 23, no. 2 (2022): 144. https://doi.org/10.3892/etm.2021.11067
Copy and paste a formatted citation
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Spandidos Publications style
Wang X, Zhang Y, Wuyun K and Gong H: Therapeutic effect and mechanism of 4‑phenyl butyric acid on renal ischemia‑reperfusion injury in mice. Exp Ther Med 23: 144, 2022.
APA
Wang, X., Zhang, Y., Wuyun, K., & Gong, H. (2022). Therapeutic effect and mechanism of 4‑phenyl butyric acid on renal ischemia‑reperfusion injury in mice. Experimental and Therapeutic Medicine, 23, 144. https://doi.org/10.3892/etm.2021.11067
MLA
Wang, X., Zhang, Y., Wuyun, K., Gong, H."Therapeutic effect and mechanism of 4‑phenyl butyric acid on renal ischemia‑reperfusion injury in mice". Experimental and Therapeutic Medicine 23.2 (2022): 144.
Chicago
Wang, X., Zhang, Y., Wuyun, K., Gong, H."Therapeutic effect and mechanism of 4‑phenyl butyric acid on renal ischemia‑reperfusion injury in mice". Experimental and Therapeutic Medicine 23, no. 2 (2022): 144. https://doi.org/10.3892/etm.2021.11067
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