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p‑Coumaric acid suppresses reactive oxygen species‑induced senescence in nucleus pulposus cells

  • Authors:
    • Kunkun Sheng
    • Yan Li
    • Zhan Wang
    • Kai Hang
    • Zhaoming Ye
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China, Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China
    Copyright: © Sheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 183
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    Published online on: December 30, 2021
       https://doi.org/10.3892/etm.2021.11106
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Abstract

p‑Coumaric acid (PCA) is a phenolic acid that is widely present in numerous plants and human diets. Studies have demonstrated the antioxidant and anti‑senescence effects of PCA in different cell types. However, the anti‑senescence effects of PCA in nucleus pulposus (NP) cells have remained to be determined. In the present study, reverse transcription‑quantitative PCR was used to measure the gene expression of Cyclooxygenase‑2 (Cox‑2), inducible nitric oxide synthase (iNOS), p53, p16, aggrecan and collagen‑2 in NP cells. Immunofluorescence staining was used to evaluate the protein expression of p53, p16 and collagen‑2 in NP cells. In addition, cell cycle of NP cells was measured by flow cytometry. β‑galactosidase staining were used to investigate the senescence of NP cells. Preliminary results indicated that PCA suppressed ROS‑induced senescence in NP cells via both the p16 and p53 pathways. NP cells were pretreated with PCA at a concentration of 10 or 50 µg/ml prior to stimulation with 200 µM hydrogen peroxide (H2O2). Pretreatment with PCA significantly inhibited H2O2‑induced cell cycle arrest in a dose‑dependent manner. PCA also reduced the gene expression of Cox‑2, iNOS, p53 and p16 induced by H2O2. By contrast, aggrecan and collagen‑2 expression in NP cells was upregulated after PCA treatment. Furthermore, PCA suppressed H2O2‑induced changes in the protein expression of p16, p53 and collagen‑2. H2O2 stimulation of NP cells increased senescence‑associated β‑galactosidase (SA‑β‑gal) activities, while PCA treatment markedly reversed these SA‑β‑gal activities. Collectively, the present results indicated that PCA attenuated H2O2‑induced oxidative stress and cellular senescence, suggesting a potential therapeutic utility of PCA in intervertebral disc degeneration.
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1 

Hartvigsen J, Hancock MJ, Kongsted A, Louw Q, Ferreira ML, Genevay S, Hoy D, Karppinen J, Pransky G, Sieper J, et al: What low back pain is and why we need to pay attention. Lancet. 391:2356–2367. 2018.PubMed/NCBI View Article : Google Scholar

2 

Gille O, Bouloussa H, Mazas S, Vergari C, Challier V, Vital JM, Coudert P and Ghailane S: A new classification system for degenerative spondylolisthesis of the lumbar spine. Eur Spine J. 26:3096–3105. 2017.PubMed/NCBI View Article : Google Scholar

3 

Vos T, Flaxman AD, Naghavi M, Lozano R, Michaud C, Ezzati M, Shibuya K, Salomon JA, Abdalla S, Aboyans V, et al: Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990-2010: A systematic analysis for the global burden of disease study 2010. Lancet. 380:2163–2196. 2012.PubMed/NCBI View Article : Google Scholar

4 

Andersson GB: Epidemiological features of chronic low-back pain. Lancet. 354:581–585. 1999.PubMed/NCBI View Article : Google Scholar

5 

Feng C, Liu H, Yang M, Zhang Y, Huang B and Zhou Y: Disc cell senescence in intervertebral disc degeneration: Causes and molecular pathways. Cell Cycle. 15:1674–1684. 2016.PubMed/NCBI View Article : Google Scholar

6 

Zhang Y, Yang B, Wang J, Cheng F, Shi K, Ying L, Wang C, Xia K, Huang X, Gong Z, et al: Cell senescence: A nonnegligible cell state under survival stress in pathology of intervertebral disc degeneration. Oxid Med Cell Longev. 2020(9503562)2020.PubMed/NCBI View Article : Google Scholar

7 

Tang N, Dong Y, Chen C and Zhao H: Anisodamine maintains the stability of intervertebral disc tissue by inhibiting the senescence of nucleus pulposus cells and degradation of extracellular matrix via interleukin-6/janus kinases/signal transducer and activator of transcription 3 pathway. Front Pharmacol. 11(519172)2020.PubMed/NCBI View Article : Google Scholar

8 

Machino M, Yukawa Y, Imagama S, Ito K, Katayama Y, Matsumoto T, Inoue T, Ouchida J, Tomita K, Ishiguro N and Kato F: Age-related and degenerative changes in the osseous anatomy, alignment, and range of motion of the cervical spine: A comparative study of radiographic data from 1016 patients with cervical spondylotic myelopathy and 1230 asymptomatic subjects. Spine (Phila Pa 1976). 41:476–482. 2016.PubMed/NCBI View Article : Google Scholar

9 

Lee DC, Adams CS, Albert TJ, Shapiro IM, Evans SM and Koch CJ: In situ oxygen utilization in the rat intervertebral disc. J Anat. 210:294–303. 2007.PubMed/NCBI View Article : Google Scholar

10 

Passos JF, Nelson G, Wang C, Richter T, Simillion C, Proctor CJ, Miwa S, Olijslagers S, Hallinan J, Wipat A, et al: Feedback between p21 and reactive oxygen production is necessary for cell senescence. Mol Syst Biol. 6(347)2010.PubMed/NCBI View Article : Google Scholar

11 

Deniz GY and Altun S: Evaluation of nickel-induced brain injuries in rats via oxidative stress and apoptosis: Attenuating effects of hyperoside. Turk J Zool. 44:104–113. 2020.

12 

Tian Y, Bao Z, Ji Y, Mei X and Yang H: Epigallocatechin-3-gallate protects H2O2-induced nucleus pulposus cell apoptosis and inflammation by inhibiting cGAS/sting/NLRP3 activation. Drug Des Devel Ther. 14:2113–2122. 2020.PubMed/NCBI View Article : Google Scholar

13 

Tesanovic K, Pejin B, Sibul F, Matavulj M, Rašeta M, Janjušević L and Karaman M: A comparative overview of antioxidative properties and phenolic profiles of different fungal origins: Fruiting bodies and submerged cultures of Coprinus comatus and Coprinellus truncorum. J Food Sci Technol. 54:430–438. 2017.PubMed/NCBI View Article : Google Scholar

14 

Janjušević L, Karaman M, Šibul F, Tommonaro G, Iodice C, Jakovljević D and Pejin B: The lignicolous fungus trametes versicolor (L.) Lloyd (1920): A promising natural source of antiradical and AChE inhibitory agents. J Enzyme Inhib Med Chem. 32:355–362. 2017.PubMed/NCBI View Article : Google Scholar

15 

An SM, Koh JS and Boo YC: p-Coumaric acid not only inhibits human tyrosinase activity in vitro but also melanogenesis in cells exposed to UVB. Phytother Res. 24:1175–1180. 2010.PubMed/NCBI View Article : Google Scholar

16 

Luceri C, Giannini L, Lodovici M, Antonucci E, Abbate R, Masini E and Dolara P: p-Coumaric acid, a common dietary phenol, inhibits platelet activity in vitro and in vivo. Br J Nutr. 97:458–463. 2007.PubMed/NCBI View Article : Google Scholar

17 

Navaneethan D and Rasool M: p-Coumaric acid, a common dietary polyphenol, protects cadmium chloride-induced nephrotoxicity in rats. Ren Fail. 36:244–251. 2014.PubMed/NCBI View Article : Google Scholar

18 

Pragasam SJ, Venkatesan V and Rasool M: Immunomodulatory and anti-inflammatory effect of p-coumaric acid, a common dietary polyphenol on experimental inflammation in rats. Inflammation. 36:169–176. 2013.PubMed/NCBI View Article : Google Scholar

19 

Huang X, You Y, Xi Y, Ni B, Chu X, Zhang R and You H: p-Coumaric acid attenuates IL-1β-induced inflammatory responses and cellular senescence in rat chondrocytes. Inflammation. 43:619–628. 2020.PubMed/NCBI View Article : Google Scholar

20 

Griffith JF, Wang YX, Antonio GE, Choi KC, Yu A, Ahuja AT and Leung PC: Modified pfirrmann grading system for lumbar intervertebral disc degeneration. Spine (Phila Pa 1976). 32:E708–E712. 2007.PubMed/NCBI View Article : Google Scholar

21 

Chen QM, Liu J and Merrett JB: Apoptosis or senescence-like growth arrest: Influence of cell-cycle position, p53, p21 and bax in H2O2 response of normal human fibroblasts. Biochem J. 347:543–551. 2000.PubMed/NCBI View Article : Google Scholar

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

23 

Childs BG, Durik M, Baker DJ and van Deursen JM: Cellular senescence in aging and age-related disease: From mechanisms to therapy. Nat Med. 21:1424–1435. 2015.PubMed/NCBI View Article : Google Scholar

24 

Kirkland JL and Tchkonia T: Cellular senescence: A translational perspective. EBioMedicine. 21:21–28. 2017.PubMed/NCBI View Article : Google Scholar

25 

Kepler CK, Ponnappan RK, Tannoury CA, Risbud MV and Anderson DG: The molecular basis of intervertebral disc degeneration. Spine J. 13:318–330. 2013.PubMed/NCBI View Article : Google Scholar

26 

de Magalhaes JP and Passos JF: Stress, cell senescence and organismal ageing. Mech Ageing Dev. 170:2–9. 2018.PubMed/NCBI View Article : Google Scholar

27 

Xing QJ, Liang QQ, Bian Q, Ding DF, Cui XJ, Shi Q and Wang YJ: Leg amputation accelerates senescence of rat lumbar intervertebral discs. Spine (Phila Pa 1976). 35:E1253–E1261. 2010.PubMed/NCBI View Article : Google Scholar

28 

Munoz-Espin D and Serrano M: Cellular senescence: From physiology to pathology. Nat Rev Mol Cell Biol. 15:482–496. 2014.PubMed/NCBI View Article : Google Scholar

29 

Chicas A, Wang X, Zhang C, McCurrach M, Zhao Z, Mert O, Dickins RA, Narita M, Zhang M and Lowe SW: Dissecting the unique role of the retinoblastoma tumor suppressor during cellular senescence. Cancer Cell. 17:376–387. 2010.PubMed/NCBI View Article : Google Scholar

30 

Rass U, Ahel I and West SC: Defective DNA repair and neurodegenerative disease. Cell. 130:991–1004. 2007.PubMed/NCBI View Article : Google Scholar

31 

Liu D and Xu Y: p53, oxidative stress, and aging. Antioxid Redox Signal. 15:1669–1678. 2011.PubMed/NCBI View Article : Google Scholar

32 

Mijit M, Caracciolo V, Melillo A, Amicarelli F and Giordano A: Role of p53 in the regulation of cellular senescence. Biomolecules. 10(420)2020.PubMed/NCBI View Article : Google Scholar

33 

Vousden KH and Prives C: Blinded by the light: The growing complexity of p53. Cell. 137:413–431. 2009.PubMed/NCBI View Article : Google Scholar

34 

Spallarossa P, Altieri P, Aloi C, Garibaldi S, Barisione C, Ghigliotti G, Fugazza G, Barsotti A and Brunelli C: Doxorubicin induces senescence or apoptosis in rat neonatal cardiomyocytes by regulating the expression levels of the telomere binding factors 1 and 2. Am J Physiol Heart Circ Physiol. 297:H2169–H2181. 2009.PubMed/NCBI View Article : Google Scholar

35 

Rafiee Z, Moaiedi MZ, Gorji AV and Mansouri E: P-coumaric acid mitigates doxorubicin-induced nephrotoxicity through suppression of oxidative stress, inflammation and apoptosis. Arch Med Res. 51:32–40. 2020.PubMed/NCBI View Article : Google Scholar

36 

Xie W, Zhang S, Lei F, Ouyang X and Du L: Ananas comosus L. Leaf phenols and p-coumaric acid regulate liver fat metabolism by upregulating CPT-1 expression. Evid Based Complement Alternat Med. 2014(903258)2014.PubMed/NCBI View Article : Google Scholar

37 

Abazari MF, Nasiri N, Karizi SZ, Nejati F, Haghi-Aminjan H, Norouzi S, Piri P, Estakhr L, Faradonbeh DR and Kohandani M: An updated review of various medicinal applications of p-coumaric acid: From antioxidative and anti-inflammatory properties to effects on cell cycle and proliferation. Mini Rev Med Chem. 21:2187–2201. 2021.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Sheng K, Li Y, Wang Z, Hang K and Ye Z: p‑Coumaric acid suppresses reactive oxygen species‑induced senescence in nucleus pulposus cells. Exp Ther Med 23: 183, 2022.
APA
Sheng, K., Li, Y., Wang, Z., Hang, K., & Ye, Z. (2022). p‑Coumaric acid suppresses reactive oxygen species‑induced senescence in nucleus pulposus cells. Experimental and Therapeutic Medicine, 23, 183. https://doi.org/10.3892/etm.2021.11106
MLA
Sheng, K., Li, Y., Wang, Z., Hang, K., Ye, Z."p‑Coumaric acid suppresses reactive oxygen species‑induced senescence in nucleus pulposus cells". Experimental and Therapeutic Medicine 23.2 (2022): 183.
Chicago
Sheng, K., Li, Y., Wang, Z., Hang, K., Ye, Z."p‑Coumaric acid suppresses reactive oxygen species‑induced senescence in nucleus pulposus cells". Experimental and Therapeutic Medicine 23, no. 2 (2022): 183. https://doi.org/10.3892/etm.2021.11106
Copy and paste a formatted citation
x
Spandidos Publications style
Sheng K, Li Y, Wang Z, Hang K and Ye Z: p‑Coumaric acid suppresses reactive oxygen species‑induced senescence in nucleus pulposus cells. Exp Ther Med 23: 183, 2022.
APA
Sheng, K., Li, Y., Wang, Z., Hang, K., & Ye, Z. (2022). p‑Coumaric acid suppresses reactive oxygen species‑induced senescence in nucleus pulposus cells. Experimental and Therapeutic Medicine, 23, 183. https://doi.org/10.3892/etm.2021.11106
MLA
Sheng, K., Li, Y., Wang, Z., Hang, K., Ye, Z."p‑Coumaric acid suppresses reactive oxygen species‑induced senescence in nucleus pulposus cells". Experimental and Therapeutic Medicine 23.2 (2022): 183.
Chicago
Sheng, K., Li, Y., Wang, Z., Hang, K., Ye, Z."p‑Coumaric acid suppresses reactive oxygen species‑induced senescence in nucleus pulposus cells". Experimental and Therapeutic Medicine 23, no. 2 (2022): 183. https://doi.org/10.3892/etm.2021.11106
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