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HSP60 participates in the anti‑glioma effects of curcumin

  • Authors:
    • Fengchen Bi
    • Junyan Wang
    • Xiaomin Zheng
    • Jingjing Xiao
    • Chai Zhi
    • Jinhai Gu
    • Yumei Zhang
    • Juan Li
    • Zhenhua Miao
    • Yin Wang
    • Yunhong Li
  • View Affiliations / Copyright

    Affiliations: Ningxia Key Laboratory of Cerebrocranial Diseases, Ningxia Medical University, Yinchuan, Ningxia Hui 750004, P.R. China, Basic Medical School of Ningxia Medical University, Yinchuan, Ningxia Hui 750004, P.R. China, School of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia Hui 750004, P.R. China
    Copyright: © Bi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 204
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    Published online on: January 11, 2021
       https://doi.org/10.3892/etm.2021.9637
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Abstract

The chaperone protein heat shock protein 60 (HSP60) is considered a tumor promoter in several types of primary human tumors, where it orchestrates a broad range of survival programs. Curcumin (CCM) is well‑established to exhibit several anticancer properties with an excellent safety profile. Our previous study showed that CCM suppresses extracellular HSP60 expression, which is typically released by activated microglia, and acts as an inflammatory factor by binding to Toll‑like receptor 4 (TLR‑4) on the cell membrane. The present study assessed whether CCM exerted its anti‑neuroglioma effects on U87 cells via inhibition of HSP60/TLR‑4 signaling, similar to that in microglia. The results demonstrated that CCM significantly inhibited the viability and invasive capacity of neuroglioma U87 cells as evidenced by a Cell Counting Kit‑8 assay. Western blotting and ELISA results showed that CCM decreased the expression of HSP60 and its transcriptional factor, heat shock factor 1, and reduced HSP60 release. Accordingly, TLR‑4, as the target of HSP60, and its downstream signaling proteins myeloid differentiation primary response 88 (MYD88), NF‑κB, inducible nitric oxide synthase and cytokines IL‑1β and IL‑6 were downregulated by CCM. The expression levels of apoptotic factors associated with NF‑κB activation, including TNF‑α and caspase‑3 were increased in U87 cells by CCM treatment, while p53 expression, a tumor suppressor, was shown to be decreased. Based on the results of the present study, CCM may exert its anti‑tumor effects in U87 cells by inhibiting the HSP60/TLR‑4/MYD88/NF‑κB pathway and inducing tumor cell apoptosis. Thus, CCM may be used as a potential therapy for the clinical treatment of neuroglioma.
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Copy and paste a formatted citation
Spandidos Publications style
Bi F, Wang J, Zheng X, Xiao J, Zhi C, Gu J, Zhang Y, Li J, Miao Z, Wang Y, Wang Y, et al: HSP60 participates in the anti‑glioma effects of curcumin. Exp Ther Med 21: 204, 2021.
APA
Bi, F., Wang, J., Zheng, X., Xiao, J., Zhi, C., Gu, J. ... Li, Y. (2021). HSP60 participates in the anti‑glioma effects of curcumin. Experimental and Therapeutic Medicine, 21, 204. https://doi.org/10.3892/etm.2021.9637
MLA
Bi, F., Wang, J., Zheng, X., Xiao, J., Zhi, C., Gu, J., Zhang, Y., Li, J., Miao, Z., Wang, Y., Li, Y."HSP60 participates in the anti‑glioma effects of curcumin". Experimental and Therapeutic Medicine 21.3 (2021): 204.
Chicago
Bi, F., Wang, J., Zheng, X., Xiao, J., Zhi, C., Gu, J., Zhang, Y., Li, J., Miao, Z., Wang, Y., Li, Y."HSP60 participates in the anti‑glioma effects of curcumin". Experimental and Therapeutic Medicine 21, no. 3 (2021): 204. https://doi.org/10.3892/etm.2021.9637
Copy and paste a formatted citation
x
Spandidos Publications style
Bi F, Wang J, Zheng X, Xiao J, Zhi C, Gu J, Zhang Y, Li J, Miao Z, Wang Y, Wang Y, et al: HSP60 participates in the anti‑glioma effects of curcumin. Exp Ther Med 21: 204, 2021.
APA
Bi, F., Wang, J., Zheng, X., Xiao, J., Zhi, C., Gu, J. ... Li, Y. (2021). HSP60 participates in the anti‑glioma effects of curcumin. Experimental and Therapeutic Medicine, 21, 204. https://doi.org/10.3892/etm.2021.9637
MLA
Bi, F., Wang, J., Zheng, X., Xiao, J., Zhi, C., Gu, J., Zhang, Y., Li, J., Miao, Z., Wang, Y., Li, Y."HSP60 participates in the anti‑glioma effects of curcumin". Experimental and Therapeutic Medicine 21.3 (2021): 204.
Chicago
Bi, F., Wang, J., Zheng, X., Xiao, J., Zhi, C., Gu, J., Zhang, Y., Li, J., Miao, Z., Wang, Y., Li, Y."HSP60 participates in the anti‑glioma effects of curcumin". Experimental and Therapeutic Medicine 21, no. 3 (2021): 204. https://doi.org/10.3892/etm.2021.9637
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