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Article

The vasodilatory mechanism of nitric oxide and hydrogen sulfide in the human mesenteric artery in patients with colorectal cancer

  • Authors:
    • Awat Y. Hassan
    • Ismail M. Maulood
    • Abbas Salihi
  • View Affiliations / Copyright

    Affiliations: Department of Biology, College of Science, Salahaddin University‑Erbil, Erbil, Kurdistan Region 44001, Iraq
  • Article Number: 214
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    Published online on: January 15, 2021
       https://doi.org/10.3892/etm.2021.9646
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Abstract

Recent studies have focused on the role of gasotransmitters in cancer progression and prevention. Therefore, the current study was designed to explore the vasodilator activity of NO and H2S in the human mesenteric arteries of patients with colorectal cancer (CRC) via the activation of K+ channels. A total of two sets of experiments were established for the current investigation. Blood samples from patients with CRC were obtained to detect serum levels of endocan and malondialdehyde (MDA). The role of K+ channels in mediating the vasodilation of the human mesenteric artery in response to sodium nitroprusside (SNP) and sodium disulfide (Na2S) was assessed. The level of serum endocan was indicated to be decreased in patients with CRC compared with healthy individuals, while the level of serum MDA remained unaltered between groups. The arterial rings pre‑contracted with norepinephrine were first relaxed by the cumulative addition of increasing concentrations of either SNP (30 nM‑30 µM) or (1‑6 mM). Maximal relaxation rates were then calculated at 15 min intervals for 60 min. Pre‑incubation of arterial rings for 20 min with individual K+ channel blockers was indicated to significantly reduce SNP‑ and Na2S‑induced relaxation at different time points. Pre‑treatment of L‑nitro‑arginine methyl ester did not alter vasodilation that was induced by Na2S. Furthermore, vasodilation of the CRC mesenteric artery was not altered by the synergistic application of SNP and Na2S, while pre‑incubation of arterial rings with D,L‑propargylglycine significantly enhanced vasodilation induced by SNP. These results indicated that endothelial dysfunction and oxidative stress do not serve roles in the pathogenesis of CRC. The dilatory mechanisms of NO and H2S in mesenteric arteries of patients with CRC were K+ channel‑ and time‑dependent, and the activity of cystathionine γ‑lyase enzyme inhibited the ability of exogenous NO in vasodilation processes.
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Copy and paste a formatted citation
Spandidos Publications style
Hassan AY, Maulood IM and Salihi A: The vasodilatory mechanism of nitric oxide and hydrogen sulfide in the human mesenteric artery in patients with colorectal cancer. Exp Ther Med 21: 214, 2021.
APA
Hassan, A.Y., Maulood, I.M., & Salihi, A. (2021). The vasodilatory mechanism of nitric oxide and hydrogen sulfide in the human mesenteric artery in patients with colorectal cancer. Experimental and Therapeutic Medicine, 21, 214. https://doi.org/10.3892/etm.2021.9646
MLA
Hassan, A. Y., Maulood, I. M., Salihi, A."The vasodilatory mechanism of nitric oxide and hydrogen sulfide in the human mesenteric artery in patients with colorectal cancer". Experimental and Therapeutic Medicine 21.3 (2021): 214.
Chicago
Hassan, A. Y., Maulood, I. M., Salihi, A."The vasodilatory mechanism of nitric oxide and hydrogen sulfide in the human mesenteric artery in patients with colorectal cancer". Experimental and Therapeutic Medicine 21, no. 3 (2021): 214. https://doi.org/10.3892/etm.2021.9646
Copy and paste a formatted citation
x
Spandidos Publications style
Hassan AY, Maulood IM and Salihi A: The vasodilatory mechanism of nitric oxide and hydrogen sulfide in the human mesenteric artery in patients with colorectal cancer. Exp Ther Med 21: 214, 2021.
APA
Hassan, A.Y., Maulood, I.M., & Salihi, A. (2021). The vasodilatory mechanism of nitric oxide and hydrogen sulfide in the human mesenteric artery in patients with colorectal cancer. Experimental and Therapeutic Medicine, 21, 214. https://doi.org/10.3892/etm.2021.9646
MLA
Hassan, A. Y., Maulood, I. M., Salihi, A."The vasodilatory mechanism of nitric oxide and hydrogen sulfide in the human mesenteric artery in patients with colorectal cancer". Experimental and Therapeutic Medicine 21.3 (2021): 214.
Chicago
Hassan, A. Y., Maulood, I. M., Salihi, A."The vasodilatory mechanism of nitric oxide and hydrogen sulfide in the human mesenteric artery in patients with colorectal cancer". Experimental and Therapeutic Medicine 21, no. 3 (2021): 214. https://doi.org/10.3892/etm.2021.9646
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