Open Access

Insulin-like growth factor-1 improves postoperative cognitive dysfunction following splenectomy in aged rats

  • Authors:
    • Bin Wang
    • Xu Lin
    • Jiahui Zhou
    • Chunhui Xie
    • Chuan Li
    • Rui Dong
    • Gaofeng Zhang
    • Xiaopeng Sun
    • Mingshan Wang
    • Yanlin Bi
  • View Affiliations

  • Published online on: January 15, 2021     https://doi.org/10.3892/etm.2021.9647
  • Article Number: 215
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Postoperative cognitive dysfunction (POCD) is a serious complication following anesthesia and operations in aged patients undergoing surgical intervention. It is characterized by temporary or permanent cognitive decline, memory impairment and deterioration in language comprehension and social adaption ability. Therefore, the development of POCD prevention and treatment tools has become an area of interest. The current study assessed the therapeutic effects of insulin-like growth factor-1 (IGF-1) on POCD in aged rats and explored the underlying mechanisms. Model rats underwent splenectomy under 1.5-2% isoflurane and mechanical ventilation. IGF-1 (50 µg/kg) was diluted in normal saline and administered by abdominal hypodermic injection daily from the operation to day 7 post-operation. Following splenectomy, the animals showed marked cognitive impairment as determined by the Morris water maze test. Hippocampal protein levels of amyloid precursor protein (APP), β-site APP-cleaving enzyme-1 (BACE-1), amyloid-β (Aβ), capase3, Bax and Bcl-2 were assessed by immunoblotting. Neuronal apoptosis in the hippocampus was analyzed using a TUNEL assay. The results demonstrated that the levels of APP, BACE-1, Aβ, caspase3 and Bax were increased following splenectomy, while the levels of Bcl2 were reduced at days 1, 3 and 7 post-operation in aged rats. However, IGF-1 downregulated APP, BACE-1, Aβ, capase3 and Bax, and upregulated Bcl2 at these time points following splenectomy. TUNEL staining revealed that administration of IGF-1 significantly reduced neuronal apoptosis in the hippocampal CA1 region following splenectomy. These results indicated that IGF-1 decreased Aβ-protein production and inhibited neuronal apoptosis in the hippocampus following splenectomy, subsequently alleviating POCD.
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March-2021
Volume 21 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang B, Lin X, Zhou J, Xie C, Li C, Dong R, Zhang G, Sun X, Wang M, Bi Y, Bi Y, et al: Insulin-like growth factor-1 improves postoperative cognitive dysfunction following splenectomy in aged rats. Exp Ther Med 21: 215, 2021
APA
Wang, B., Lin, X., Zhou, J., Xie, C., Li, C., Dong, R. ... Bi, Y. (2021). Insulin-like growth factor-1 improves postoperative cognitive dysfunction following splenectomy in aged rats. Experimental and Therapeutic Medicine, 21, 215. https://doi.org/10.3892/etm.2021.9647
MLA
Wang, B., Lin, X., Zhou, J., Xie, C., Li, C., Dong, R., Zhang, G., Sun, X., Wang, M., Bi, Y."Insulin-like growth factor-1 improves postoperative cognitive dysfunction following splenectomy in aged rats". Experimental and Therapeutic Medicine 21.3 (2021): 215.
Chicago
Wang, B., Lin, X., Zhou, J., Xie, C., Li, C., Dong, R., Zhang, G., Sun, X., Wang, M., Bi, Y."Insulin-like growth factor-1 improves postoperative cognitive dysfunction following splenectomy in aged rats". Experimental and Therapeutic Medicine 21, no. 3 (2021): 215. https://doi.org/10.3892/etm.2021.9647