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Circular RNA expression profile in human primary multiple intracranial aneurysm

  • Authors:
    • Huimin Cao
    • Jia Chen
    • Xiaoyan Lai
    • Tianqin Liu
    • Ping Qiu
    • Shuanglin Que
    • Yanming Huang
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, Longyan First Hospital Affiliated to Fujian Medical University, Longyan, Fujian 364000, P.R. China, Department of Neurosurgery, Longyan First Hospital Affiliated to Fujian Medical University, Longyan, Fujian 364000, P.R. China
    Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 239
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    Published online on: January 21, 2021
       https://doi.org/10.3892/etm.2021.9670
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Abstract

Primary multiple intracranial aneurysm (MIA) is a vascular disease that frequently leads to fatal vascular rupture and subarachnoid hemorrhage. However, the epigenetic regulation associated with MIA has remained largely elusive. Circular RNAs (circRNAs) serve important roles in cardiovascular diseases; however, their association with MIA has remained to be investigated. The present study initially aimed to explore novel mechanisms of MIA through examining circRNA expression profiles. Comprehensive circRNA expression profiles were detected by RNA sequencing (RNA‑Seq) in human peripheral blood mononuclear cells. The RNA‑Seq results were validated by reverse transcription‑quantitative PCR. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses suggested the functions of these circRNAs. A competing endogenous RNA network was constructed to reveal the circRNA‑microRNA‑mRNA relationship. Among the 3,328 differentially expressed circRNAs between the MIA and matched control groups, 60 exhibited significant expression changes (|log2 fold change|≥2; P<0.05). Among these 60 circRNAs, 20 were upregulated, while the other 40 were downregulated. A number of downregulated circRNAs were involved in inflammation. The most significant KEGG pathway was ‘leukocyte transendothelial migration’. The circRNAs Homo sapiens (hsa)_circ_0135895, hsa_circ_0000682 and hsa_circ_0000690, which were also associated with the above‑mentioned pathway, were indicated to be able to regulate protein tyrosine kinase 2, protein kinase Cβ and integrin subunit αL, respectively. To the best of our knowledge, the present study was the first to perform a circRNA sequencing analysis of MIA. The results specifically predicted the regulatory role of circRNAs in the pathogenesis of MIA. ‘Leukocyte transendothelial migration’ may be critical for the pathogenesis of MIA.
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Copy and paste a formatted citation
Spandidos Publications style
Cao H, Chen J, Lai X, Liu T, Qiu P, Que S and Huang Y: Circular RNA expression profile in human primary multiple intracranial aneurysm. Exp Ther Med 21: 239, 2021.
APA
Cao, H., Chen, J., Lai, X., Liu, T., Qiu, P., Que, S., & Huang, Y. (2021). Circular RNA expression profile in human primary multiple intracranial aneurysm. Experimental and Therapeutic Medicine, 21, 239. https://doi.org/10.3892/etm.2021.9670
MLA
Cao, H., Chen, J., Lai, X., Liu, T., Qiu, P., Que, S., Huang, Y."Circular RNA expression profile in human primary multiple intracranial aneurysm". Experimental and Therapeutic Medicine 21.3 (2021): 239.
Chicago
Cao, H., Chen, J., Lai, X., Liu, T., Qiu, P., Que, S., Huang, Y."Circular RNA expression profile in human primary multiple intracranial aneurysm". Experimental and Therapeutic Medicine 21, no. 3 (2021): 239. https://doi.org/10.3892/etm.2021.9670
Copy and paste a formatted citation
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Spandidos Publications style
Cao H, Chen J, Lai X, Liu T, Qiu P, Que S and Huang Y: Circular RNA expression profile in human primary multiple intracranial aneurysm. Exp Ther Med 21: 239, 2021.
APA
Cao, H., Chen, J., Lai, X., Liu, T., Qiu, P., Que, S., & Huang, Y. (2021). Circular RNA expression profile in human primary multiple intracranial aneurysm. Experimental and Therapeutic Medicine, 21, 239. https://doi.org/10.3892/etm.2021.9670
MLA
Cao, H., Chen, J., Lai, X., Liu, T., Qiu, P., Que, S., Huang, Y."Circular RNA expression profile in human primary multiple intracranial aneurysm". Experimental and Therapeutic Medicine 21.3 (2021): 239.
Chicago
Cao, H., Chen, J., Lai, X., Liu, T., Qiu, P., Que, S., Huang, Y."Circular RNA expression profile in human primary multiple intracranial aneurysm". Experimental and Therapeutic Medicine 21, no. 3 (2021): 239. https://doi.org/10.3892/etm.2021.9670
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