MicroRNA‑129‑5p promotes proliferation and metastasis of hepatocellular carcinoma by regulating the BMP2 gene

  • Authors:
    • Zengyao Liu
    • Jiefei Sun
    • Xiaochun Wang
    • Zhenyuan Cao
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  • Published online on: January 25, 2021     https://doi.org/10.3892/etm.2021.9688
  • Article Number: 257
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Abstract

Hepatocellular carcinoma (HCC) is a malignant tumor that poses a serious threat to human health. Due to its occult onset and rapid development, HCC is a challenge to diagnose early and effectively treat, and thus patients with HCC often have an unfavorable prognosis. MicroRNA (miR)‑129 and its target gene play an important role in the regulation of various diseases. Therefore, the aim of the present study was to investigate the role and mechanism of action for miR‑129‑5p in the development of HCC. Quantitative results of clinical samples analyzed using reverse transcription‑quantitative PCR suggested that miR‑129‑5p had a significantly lower expression level in tumoral tissues compared with corresponding peritumoral tissues. Overexpression of miR‑129‑5p in HCC cells was performed using a transfection technique, followed by MTT, Transwell, invasion and wound healing assays to detect the effect of miR‑129‑5p on the cell cytotoxicity and metastasis of liver cancer in vitro. The downstream target gene of miR‑129‑5p, bone morphogenetic protein 2 (BMP2), was determined using a luciferase reporter assay. Overexpression of miR‑129‑5p played a vital role in decreasing cytotoxicity and promoting metastasis of HCC, which may be attributed to its inhibitory effect on the expression of its target gene, BMP2. In clinical samples, miR‑129‑5p expression levels were found to be negatively correlated with BMP2 and closely associated with HCC metastasis and infiltration. Collectively, the results suggested that miR‑129‑5p may contribute to proliferation and metastasis of HCC through its target gene, BMP2, and thus may be a potential novel therapeutic target for the treatment of HCC.
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March-2021
Volume 21 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu Z, Sun J, Wang X and Cao Z: MicroRNA‑129‑5p promotes proliferation and metastasis of hepatocellular carcinoma by regulating the BMP2 gene. Exp Ther Med 21: 257, 2021
APA
Liu, Z., Sun, J., Wang, X., & Cao, Z. (2021). MicroRNA‑129‑5p promotes proliferation and metastasis of hepatocellular carcinoma by regulating the BMP2 gene. Experimental and Therapeutic Medicine, 21, 257. https://doi.org/10.3892/etm.2021.9688
MLA
Liu, Z., Sun, J., Wang, X., Cao, Z."MicroRNA‑129‑5p promotes proliferation and metastasis of hepatocellular carcinoma by regulating the BMP2 gene". Experimental and Therapeutic Medicine 21.3 (2021): 257.
Chicago
Liu, Z., Sun, J., Wang, X., Cao, Z."MicroRNA‑129‑5p promotes proliferation and metastasis of hepatocellular carcinoma by regulating the BMP2 gene". Experimental and Therapeutic Medicine 21, no. 3 (2021): 257. https://doi.org/10.3892/etm.2021.9688