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Green tea extract synergistically enhances the effectiveness of an antiresorptive drug on management of osteoporosis induced by ovariectomy in a rat model

  • Authors:
    • Wing Sum Siu
    • Chun Hay Ko
    • Hoi Ting Shiu
    • Kai Kai Li
    • Wai Ting Shum
    • Ping Chung Leung
    • Jin Fang Zhang
  • View Affiliations / Copyright

    Affiliations: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, SAR, P.R. China, Department of Orthopaedics and Traumatology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, SAR, P.R. China
    Copyright: © Siu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 328
    |
    Published online on: February 5, 2021
       https://doi.org/10.3892/etm.2021.9759
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Abstract

Antiresorptive drugs are effective for reducing bone loss in postmenopausal women, but their long‑term application may be associated with adverse effects. The present study aimed to investigate the potential in vivo synergistic effects of green tea extract (GTE) and alendronate or raloxifene on the management of osteoporosis. Ovariectomized rats were fed orally with GTE, alendronate and raloxifene at different concentrations and various combinations for 4 weeks. Bone mineral density (BMD) at the lumbar spine, femur and tibia was monitored weekly using peripheral quantitative computed tomography. Bone microarchitecture in the left distal femur was analyzed using micro‑CT, while serum biochemical levels were measured using ELISA kits at the end of the study. GTE alone effectively mitigated BMD loss and improved bone microarchitecture in rats. The co‑administration of GTE and alendronate increased total BMD in the lumbar spine, femur and tibia. Particularly, GTE synergistically enhanced the effect of alendronate at a low dose on bone microarchitecture and decreased serum tartrate‑resistant acid phosphatase. These findings imply that the dosage of certain antiresorptive agents could be reduced when they are administrated simultaneously with GTE, so that their adverse effects are minimized. The findings may be used to support the development of a new synergistic intervention between food therapy and pharmacotherapy on the management of osteoporosis in a long‑term basis.
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1 

Riggs BL and Melton LJ III: The worldwide problem of osteoporosis: Insights afforded by epidemiology. Bone. 17 (17 Suppl):505S–511S. 1995.PubMed/NCBI View Article : Google Scholar

2 

Lane NE: Epidemiology, etiology, and diagnosis of osteoporosis. Am J Obstet Gynecol. 194 (2 Suppl):S3–S11. 2006.PubMed/NCBI View Article : Google Scholar

3 

Varacallo MA and Fox EJ: Osteoporosis and its complications. Med Clin North Am. 98:817–831. 2014.PubMed/NCBI View Article : Google Scholar

4 

World Health Organization (WHO): WHO Scientific Group on the Assessment of Osteoporosis at Primary Health Care Level. Summary Meeting Report, Brussels, Belgium, 5-7 May 2004. WHO Press, Geneva, 2007.

5 

Kling JM, Clarke BL and Sandhu NP: Osteoporosis prevention, screening, and treatment: A review. J Womens Health (Larchmt). 23:563–572. 2014.PubMed/NCBI View Article : Google Scholar

6 

Wells GA, Cranney A, Peterson J, Boucher M, Shea B, Robinson V, Coyle D and Tugwell P: Alendronate for the primary and secondary prevention of osteoporotic fractures in postmenopausal women. Cochrane Database Syst Rev. 23(CD001155)2008.PubMed/NCBI View Article : Google Scholar

7 

Nelson HD, Haney EM, Dana T, Bougatsos C and Chou R: Screening for osteoporosis: An update for the U.S. Preventive services task force. Ann Intern Med. 153:99–111. 2010.PubMed/NCBI View Article : Google Scholar

8 

Pendrys DG and Silverman SL: Osteonecrosis of the jaws and bisphosphonates. Curr Osteoporos Rep. 6:31–38. 2008.PubMed/NCBI View Article : Google Scholar

9 

Andreopoulou P and Bockman RS: Management of postmenopausal osteoporosis. Annu Rev Med. 66:329–342. 2015.PubMed/NCBI View Article : Google Scholar

10 

Ko CH, Siu WS, Lau CP, Lau CBS, Fung KP and Leung PC: Osteoprotective effects of fructus ligustri lucidi aqueous extract in aged ovariectomized rats. Chin Med. 5(39)2010.PubMed/NCBI View Article : Google Scholar

11 

Li G, Zhang XA, Zhang JF, Chan CY, Yew DTW, He ML, Lin MCM, Leung PC and Kung HF: Ethanol extract of fructus ligustri lucidi promotes osteogenesis of mesenchymal stem cells. Phyther Res. 24:571–576. 2010.PubMed/NCBI View Article : Google Scholar

12 

Zhang JF, Li G, Chan CY, Meng CL, Lin MCM, Chen YC, He ML, Leung PC and Kung HF: Flavonoids of herba epimedii regulate osteogenesis of human mesenchymal stem cells through BMP and Wnt/β-catenin signaling pathway. Mol Cell Endocrinol. 314:70–74. 2010.PubMed/NCBI View Article : Google Scholar

13 

Hu J, Webster D, Cao J and Shao A: The safety of green tea and green tea extract consumption in adults-results of a systematic review. Regul Toxicol Pharmacol. 95:412–433. 2018.PubMed/NCBI View Article : Google Scholar

14 

Khan N and Mukhtar H: Tea and health: Studies in humans. Curr Pharm Des. 19:6141–6147. 2013.PubMed/NCBI View Article : Google Scholar

15 

Hegarty VM, May HM and Khaw KT: Tea drinking and bone mineral density in older women. Am J Clin Nutr. 71:1003–1007. 2000.PubMed/NCBI View Article : Google Scholar

16 

Ko CH, Lau KM, Choy WY and Leung PC: Effects of tea catechins, epigallocatechin, gallocatechin, and gallocatechin gallate, on bone metabolism. J Agric Food Chem. 57:7293–7297. 2009.PubMed/NCBI View Article : Google Scholar

17 

Kashiwa K, Kotobuki N, Tadokoro M, Matsumura K, Hyon SH, Yoshiya S and Ohgushi H: Effects of epigallocatechin gallate on osteogenic capability of human mesenchymal stem cells after suspension in phosphate-buffered saline. Tissue Eng Part A. 16:91–100. 2010.PubMed/NCBI View Article : Google Scholar

18 

Ko CH, Siu WS, Wong HL, Shum WT, Fung KP, Lau CBS and Leung PC: Pro-bone and antifat effects of green tea and its polyphenol, epigallocatechin, in rat mesenchymal stem cells in vitro. J Agric Food Chem. 59:9870–9876. 2011.PubMed/NCBI View Article : Google Scholar

19 

Shen CL, Wang P, Guerrieri J, Yeh JK and Wang JS: Protective effect of green tea polyphenols on bone loss in middle-aged female rats. Osteoporos Int. 19:979–990. 2008.PubMed/NCBI View Article : Google Scholar

20 

Shen CL, Yeh JK, Samathanam C, Cao JJ, Stoecker BJ, Dagda RY, Chyu MC and Wang JS: Protective actions of green tea polyphenols and alfacalcidol on bone microstructure in female rats with chronic inflammation. J Nutr Biochem. 22:673–680. 2011.PubMed/NCBI View Article : Google Scholar

21 

Shen CL, Smith BJ, Li J, Cao JJ, Song X, Newhardt MF, Corry KA, Tomison MD, Tang L, Wang JS and Chyu MC: Effect of long-term green tea polyphenol supplementation on bone architecture, turnover, and mechanical properties in middle-aged ovariectomized rats. Calcif Tissue Int. 104:285–300. 2019.PubMed/NCBI View Article : Google Scholar

22 

Dostal AM, Arikawa A, Espejo L and Kurzer MS: Long-term supplementation of green tea extract does not modify adiposity or bone mineral density in a randomized trial of overweight and obese postmenopausal women. J Nutr. 146:256–264. 2016.PubMed/NCBI View Article : Google Scholar

23 

Ko CH, Siu WS, Wong HL, Gao S, Shum WT, Lau CP, Cheng SW, Tam JCW, Hung LK, Fung KP, et al: In vivo study on the pharmacological interactions between a chinese herbal formula ELP and antiresorptive drugs to counteract osteoporosis. Evid Based Complement Altern Med. 2012(203732)2012.PubMed/NCBI View Article : Google Scholar

24 

Wu X, Xie CQ, Zhu QQ, Wang MY, Sun B, Huang YP, Shen C, An MF, Zhao YL, Wang XJ and Sheng J: Green tea (Camellia sinensis) aqueous extract alleviates postmenopausal osteoporosis in ovariectomized rats and prevents RANKL-induced osteoclastogenesis in vitro. Food Nutr Res. 8(62)2018.PubMed/NCBI View Article : Google Scholar

25 

Nair AB and Jacob S: A simple practice guide for dose conversion between animals and human. J Basic Clin Pharm. 7:27–31. 2016.PubMed/NCBI View Article : Google Scholar

26 

Halleen JM, Tiitinen SL, Ylipahkala H, Fagerlund KM and Väänänen HK: Tartrate-resistant acid phosphatase 5b (TRACP 5b) as a marker of bone resorption. Clin Lab. 52:499–509. 2006.PubMed/NCBI

27 

Rey JR, Cervino EV, Rentero ML, Crespo EC, Alvaro AO and Casillas M: Raloxifene: Mechanism of action, effects on bone tissue, and applicability in clinical traumatology practice. Open Orthop J. 3:14–21. 2009.PubMed/NCBI View Article : Google Scholar

28 

Pavone V, Testa G, Giardina SMC, Vescio A, Restivo DA and Sessa G: Pharmacological therapy of osteoporosis: A systematic current review of literature. Front Pharmacol. 8(830)2017.PubMed/NCBI View Article : Google Scholar

29 

Dulloo AG, Seydoux J, Girardier L, Chantre P and Vandermander J: Green tea and thermogenesis: Interactions between catechin-polyphenols, caffeine and sympathetic activity. Int J Obes Relat Metab Disord. 24:252–258. 2000.PubMed/NCBI View Article : Google Scholar

30 

Chacko SM, Thambi PT, Kuttan R and Nishigaki I: Beneficial effects of green tea: A literature review. Chin Med. 5(13)2010.PubMed/NCBI View Article : Google Scholar

31 

Smith A: Effects of caffeine on human behavior. Food Chem Toxicol. 40:1243–1255. 2002.PubMed/NCBI View Article : Google Scholar

32 

Cheng TO: Green tea may inhibit warfarin. Int J Cardiol. 115(236)2007.PubMed/NCBI View Article : Google Scholar

33 

Izzo AA: Interactions between herbs and conventional drugs: Overview of the clinical data. Med Princ Pract. 21:404–428. 2012.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Siu W, Ko C, Shiu H, Li K, Shum W, Leung P and Zhang J: Green tea extract synergistically enhances the effectiveness of an antiresorptive drug on management of osteoporosis induced by ovariectomy in a rat model. Exp Ther Med 21: 328, 2021.
APA
Siu, W., Ko, C., Shiu, H., Li, K., Shum, W., Leung, P., & Zhang, J. (2021). Green tea extract synergistically enhances the effectiveness of an antiresorptive drug on management of osteoporosis induced by ovariectomy in a rat model. Experimental and Therapeutic Medicine, 21, 328. https://doi.org/10.3892/etm.2021.9759
MLA
Siu, W., Ko, C., Shiu, H., Li, K., Shum, W., Leung, P., Zhang, J."Green tea extract synergistically enhances the effectiveness of an antiresorptive drug on management of osteoporosis induced by ovariectomy in a rat model". Experimental and Therapeutic Medicine 21.4 (2021): 328.
Chicago
Siu, W., Ko, C., Shiu, H., Li, K., Shum, W., Leung, P., Zhang, J."Green tea extract synergistically enhances the effectiveness of an antiresorptive drug on management of osteoporosis induced by ovariectomy in a rat model". Experimental and Therapeutic Medicine 21, no. 4 (2021): 328. https://doi.org/10.3892/etm.2021.9759
Copy and paste a formatted citation
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Spandidos Publications style
Siu W, Ko C, Shiu H, Li K, Shum W, Leung P and Zhang J: Green tea extract synergistically enhances the effectiveness of an antiresorptive drug on management of osteoporosis induced by ovariectomy in a rat model. Exp Ther Med 21: 328, 2021.
APA
Siu, W., Ko, C., Shiu, H., Li, K., Shum, W., Leung, P., & Zhang, J. (2021). Green tea extract synergistically enhances the effectiveness of an antiresorptive drug on management of osteoporosis induced by ovariectomy in a rat model. Experimental and Therapeutic Medicine, 21, 328. https://doi.org/10.3892/etm.2021.9759
MLA
Siu, W., Ko, C., Shiu, H., Li, K., Shum, W., Leung, P., Zhang, J."Green tea extract synergistically enhances the effectiveness of an antiresorptive drug on management of osteoporosis induced by ovariectomy in a rat model". Experimental and Therapeutic Medicine 21.4 (2021): 328.
Chicago
Siu, W., Ko, C., Shiu, H., Li, K., Shum, W., Leung, P., Zhang, J."Green tea extract synergistically enhances the effectiveness of an antiresorptive drug on management of osteoporosis induced by ovariectomy in a rat model". Experimental and Therapeutic Medicine 21, no. 4 (2021): 328. https://doi.org/10.3892/etm.2021.9759
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